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Versatile protein tagging in cells with split fluorescent protein
In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. The small size of these tags may reduce functional perturbation and enable signal amplification. To address their background issue, we adapt self-complem...
Autores principales: | Kamiyama, Daichi, Sekine, Sayaka, Barsi-Rhyne, Benjamin, Hu, Jeffrey, Chen, Baohui, Gilbert, Luke A., Ishikawa, Hiroaki, Leonetti, Manuel D., Marshall, Wallace F., Weissman, Jonathan S., Huang, Bo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802074/ https://www.ncbi.nlm.nih.gov/pubmed/26988139 http://dx.doi.org/10.1038/ncomms11046 |
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