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Identification and Comparative Analysis of H(2)O(2)-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches

Among major reactive oxygen species (ROS), hydrogen peroxide (H(2)O(2)) exhibits dual roles in plant metabolism. Low levels of H(2)O(2) modulate many biological/physiological processes in plants; whereas, its high level can cause damage to cell structures, having severe consequences. Thus, steady-st...

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Autores principales: Ozyigit, Ibrahim I., Filiz, Ertugrul, Vatansever, Recep, Kurtoglu, Kuaybe Y., Koc, Ibrahim, Öztürk, Münir X., Anjum, Naser A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802093/
https://www.ncbi.nlm.nih.gov/pubmed/27047498
http://dx.doi.org/10.3389/fpls.2016.00301
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author Ozyigit, Ibrahim I.
Filiz, Ertugrul
Vatansever, Recep
Kurtoglu, Kuaybe Y.
Koc, Ibrahim
Öztürk, Münir X.
Anjum, Naser A.
author_facet Ozyigit, Ibrahim I.
Filiz, Ertugrul
Vatansever, Recep
Kurtoglu, Kuaybe Y.
Koc, Ibrahim
Öztürk, Münir X.
Anjum, Naser A.
author_sort Ozyigit, Ibrahim I.
collection PubMed
description Among major reactive oxygen species (ROS), hydrogen peroxide (H(2)O(2)) exhibits dual roles in plant metabolism. Low levels of H(2)O(2) modulate many biological/physiological processes in plants; whereas, its high level can cause damage to cell structures, having severe consequences. Thus, steady-state level of cellular H(2)O(2) must be tightly regulated. Glutathione peroxidases (GPX) and ascorbate peroxidase (APX) are two major ROS-scavenging enzymes which catalyze the reduction of H(2)O(2) in order to prevent potential H(2)O(2)-derived cellular damage. Employing bioinformatics approaches, this study presents a comparative evaluation of both GPX and APX in 18 different plant species, and provides valuable insights into the nature and complex regulation of these enzymes. Herein, (a) potential GPX and APX genes/proteins from 18 different plant species were identified, (b) their exon/intron organization were analyzed, (c) detailed information about their physicochemical properties were provided, (d) conserved motif signatures of GPX and APX were identified, (e) their phylogenetic trees and 3D models were constructed, (f) protein-protein interaction networks were generated, and finally (g) GPX and APX gene expression profiles were analyzed. Study outcomes enlightened GPX and APX as major H(2)O(2)-scavenging enzymes at their structural and functional levels, which could be used in future studies in the current direction.
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spelling pubmed-48020932016-04-04 Identification and Comparative Analysis of H(2)O(2)-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches Ozyigit, Ibrahim I. Filiz, Ertugrul Vatansever, Recep Kurtoglu, Kuaybe Y. Koc, Ibrahim Öztürk, Münir X. Anjum, Naser A. Front Plant Sci Plant Science Among major reactive oxygen species (ROS), hydrogen peroxide (H(2)O(2)) exhibits dual roles in plant metabolism. Low levels of H(2)O(2) modulate many biological/physiological processes in plants; whereas, its high level can cause damage to cell structures, having severe consequences. Thus, steady-state level of cellular H(2)O(2) must be tightly regulated. Glutathione peroxidases (GPX) and ascorbate peroxidase (APX) are two major ROS-scavenging enzymes which catalyze the reduction of H(2)O(2) in order to prevent potential H(2)O(2)-derived cellular damage. Employing bioinformatics approaches, this study presents a comparative evaluation of both GPX and APX in 18 different plant species, and provides valuable insights into the nature and complex regulation of these enzymes. Herein, (a) potential GPX and APX genes/proteins from 18 different plant species were identified, (b) their exon/intron organization were analyzed, (c) detailed information about their physicochemical properties were provided, (d) conserved motif signatures of GPX and APX were identified, (e) their phylogenetic trees and 3D models were constructed, (f) protein-protein interaction networks were generated, and finally (g) GPX and APX gene expression profiles were analyzed. Study outcomes enlightened GPX and APX as major H(2)O(2)-scavenging enzymes at their structural and functional levels, which could be used in future studies in the current direction. Frontiers Media S.A. 2016-03-22 /pmc/articles/PMC4802093/ /pubmed/27047498 http://dx.doi.org/10.3389/fpls.2016.00301 Text en Copyright © 2016 Ozyigit, Filiz, Vatansever, Kurtoglu, Koc, Öztürk and Anjum. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ozyigit, Ibrahim I.
Filiz, Ertugrul
Vatansever, Recep
Kurtoglu, Kuaybe Y.
Koc, Ibrahim
Öztürk, Münir X.
Anjum, Naser A.
Identification and Comparative Analysis of H(2)O(2)-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches
title Identification and Comparative Analysis of H(2)O(2)-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches
title_full Identification and Comparative Analysis of H(2)O(2)-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches
title_fullStr Identification and Comparative Analysis of H(2)O(2)-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches
title_full_unstemmed Identification and Comparative Analysis of H(2)O(2)-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches
title_short Identification and Comparative Analysis of H(2)O(2)-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches
title_sort identification and comparative analysis of h(2)o(2)-scavenging enzymes (ascorbate peroxidase and glutathione peroxidase) in selected plants employing bioinformatics approaches
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802093/
https://www.ncbi.nlm.nih.gov/pubmed/27047498
http://dx.doi.org/10.3389/fpls.2016.00301
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