Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression

We set out to identify miR-21 targets in Jurkat cells using a high-throughput biochemical approach (10.1016/j.biochi.2014.09.021[1]). Using a specific monoclonal antibody raised against AGO2, RISC complexes were immunopurified in Jurkat cells over-expressing miR-21 following lentiviral trasduction a...

Descripción completa

Detalles Bibliográficos
Autores principales: Carissimi, Claudia, Colombo, Teresa, Azzalin, Gianluca, Cipolletta, Emanuela, Laudadio, Ilaria, Macino, Giuseppe, Fulci, Valerio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802421/
https://www.ncbi.nlm.nih.gov/pubmed/27054165
http://dx.doi.org/10.1016/j.dib.2016.02.041
Descripción
Sumario:We set out to identify miR-21 targets in Jurkat cells using a high-throughput biochemical approach (10.1016/j.biochi.2014.09.021[1]). Using a specific monoclonal antibody raised against AGO2, RISC complexes were immunopurified in Jurkat cells over-expressing miR-21 following lentiviral trasduction as well as in Jurkat control cells lines. A parallel immunoprecipitation using isotype-matched rat IgG was performed as a control. AGO2 associated mRNAs were profiled by microarray (GEO: GSE37212). AGO2 bound miRNAs were profiled by RNA-seq.

Ejemplares similares