Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression

We set out to identify miR-21 targets in Jurkat cells using a high-throughput biochemical approach (10.1016/j.biochi.2014.09.021[1]). Using a specific monoclonal antibody raised against AGO2, RISC complexes were immunopurified in Jurkat cells over-expressing miR-21 following lentiviral trasduction a...

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Autores principales: Carissimi, Claudia, Colombo, Teresa, Azzalin, Gianluca, Cipolletta, Emanuela, Laudadio, Ilaria, Macino, Giuseppe, Fulci, Valerio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802421/
https://www.ncbi.nlm.nih.gov/pubmed/27054165
http://dx.doi.org/10.1016/j.dib.2016.02.041
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author Carissimi, Claudia
Colombo, Teresa
Azzalin, Gianluca
Cipolletta, Emanuela
Laudadio, Ilaria
Macino, Giuseppe
Fulci, Valerio
author_facet Carissimi, Claudia
Colombo, Teresa
Azzalin, Gianluca
Cipolletta, Emanuela
Laudadio, Ilaria
Macino, Giuseppe
Fulci, Valerio
author_sort Carissimi, Claudia
collection PubMed
description We set out to identify miR-21 targets in Jurkat cells using a high-throughput biochemical approach (10.1016/j.biochi.2014.09.021[1]). Using a specific monoclonal antibody raised against AGO2, RISC complexes were immunopurified in Jurkat cells over-expressing miR-21 following lentiviral trasduction as well as in Jurkat control cells lines. A parallel immunoprecipitation using isotype-matched rat IgG was performed as a control. AGO2 associated mRNAs were profiled by microarray (GEO: GSE37212). AGO2 bound miRNAs were profiled by RNA-seq.
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spelling pubmed-48024212016-04-06 Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression Carissimi, Claudia Colombo, Teresa Azzalin, Gianluca Cipolletta, Emanuela Laudadio, Ilaria Macino, Giuseppe Fulci, Valerio Data Brief Data Article We set out to identify miR-21 targets in Jurkat cells using a high-throughput biochemical approach (10.1016/j.biochi.2014.09.021[1]). Using a specific monoclonal antibody raised against AGO2, RISC complexes were immunopurified in Jurkat cells over-expressing miR-21 following lentiviral trasduction as well as in Jurkat control cells lines. A parallel immunoprecipitation using isotype-matched rat IgG was performed as a control. AGO2 associated mRNAs were profiled by microarray (GEO: GSE37212). AGO2 bound miRNAs were profiled by RNA-seq. Elsevier 2016-02-24 /pmc/articles/PMC4802421/ /pubmed/27054165 http://dx.doi.org/10.1016/j.dib.2016.02.041 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Carissimi, Claudia
Colombo, Teresa
Azzalin, Gianluca
Cipolletta, Emanuela
Laudadio, Ilaria
Macino, Giuseppe
Fulci, Valerio
Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression
title Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression
title_full Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression
title_fullStr Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression
title_full_unstemmed Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression
title_short Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression
title_sort comprehensive rna dataset of ago2 associated rnas in jurkat cells following mir-21 over-expression
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802421/
https://www.ncbi.nlm.nih.gov/pubmed/27054165
http://dx.doi.org/10.1016/j.dib.2016.02.041
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