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mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines

We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) a...

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Autores principales: Gómez-Sánchez, Rubén, Yakhine-Diop, Sokhna M.S., Rodríguez-Arribas, Mario, Bravo-San Pedro, José M., Martínez-Chacón, Guadalupe, Uribe-Carretero, Elisabet, Pinheiro de Castro, Diana C.J., Pizarro-Estrella, Elisa, Fuentes, José M., González-Polo, Rosa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802425/
https://www.ncbi.nlm.nih.gov/pubmed/27054171
http://dx.doi.org/10.1016/j.dib.2016.02.085
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author Gómez-Sánchez, Rubén
Yakhine-Diop, Sokhna M.S.
Rodríguez-Arribas, Mario
Bravo-San Pedro, José M.
Martínez-Chacón, Guadalupe
Uribe-Carretero, Elisabet
Pinheiro de Castro, Diana C.J.
Pizarro-Estrella, Elisa
Fuentes, José M.
González-Polo, Rosa A.
author_facet Gómez-Sánchez, Rubén
Yakhine-Diop, Sokhna M.S.
Rodríguez-Arribas, Mario
Bravo-San Pedro, José M.
Martínez-Chacón, Guadalupe
Uribe-Carretero, Elisabet
Pinheiro de Castro, Diana C.J.
Pizarro-Estrella, Elisa
Fuentes, José M.
González-Polo, Rosa A.
author_sort Gómez-Sánchez, Rubén
collection PubMed
description We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) and p62/SQSTM1 (adaptor protein that serves as a link between LC3 and ubiquitinated substrates), (Klionsky et al., 2016) [1]. Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by quantitative PCR (qPCR). To monitor the turnover of these autophagy markers and, thus, measure the flux of this pathway, cells were under starvation conditions and/or treated with bafilomycin A1 (Baf. A1) to block fusion of autophagosomes with lysosomes.
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spelling pubmed-48024252016-04-06 mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines Gómez-Sánchez, Rubén Yakhine-Diop, Sokhna M.S. Rodríguez-Arribas, Mario Bravo-San Pedro, José M. Martínez-Chacón, Guadalupe Uribe-Carretero, Elisabet Pinheiro de Castro, Diana C.J. Pizarro-Estrella, Elisa Fuentes, José M. González-Polo, Rosa A. Data Brief Data Article We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) and p62/SQSTM1 (adaptor protein that serves as a link between LC3 and ubiquitinated substrates), (Klionsky et al., 2016) [1]. Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by quantitative PCR (qPCR). To monitor the turnover of these autophagy markers and, thus, measure the flux of this pathway, cells were under starvation conditions and/or treated with bafilomycin A1 (Baf. A1) to block fusion of autophagosomes with lysosomes. Elsevier 2016-03-09 /pmc/articles/PMC4802425/ /pubmed/27054171 http://dx.doi.org/10.1016/j.dib.2016.02.085 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Gómez-Sánchez, Rubén
Yakhine-Diop, Sokhna M.S.
Rodríguez-Arribas, Mario
Bravo-San Pedro, José M.
Martínez-Chacón, Guadalupe
Uribe-Carretero, Elisabet
Pinheiro de Castro, Diana C.J.
Pizarro-Estrella, Elisa
Fuentes, José M.
González-Polo, Rosa A.
mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines
title mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines
title_full mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines
title_fullStr mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines
title_full_unstemmed mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines
title_short mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines
title_sort mrna and protein dataset of autophagy markers (lc3 and p62) in several cell lines
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802425/
https://www.ncbi.nlm.nih.gov/pubmed/27054171
http://dx.doi.org/10.1016/j.dib.2016.02.085
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