Autophagic flux data in differentiated C2C12 myotubes following exposure to acetylcholine and caffeine

The C2C12 line of mouse myoblasts is a useful cell culture model in which to conduct in vitro analyses related to skeletal muscle. Here we present data regarding the autophagic response induced by two chemicals known to influence calcium release and contraction in skeletal muscles and C2C12 cells: a...

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Detalles Bibliográficos
Autores principales: Bloemberg, Darin, Quadrilatero, Joe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802540/
https://www.ncbi.nlm.nih.gov/pubmed/27054179
http://dx.doi.org/10.1016/j.dib.2016.03.008
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author Bloemberg, Darin
Quadrilatero, Joe
author_facet Bloemberg, Darin
Quadrilatero, Joe
author_sort Bloemberg, Darin
collection PubMed
description The C2C12 line of mouse myoblasts is a useful cell culture model in which to conduct in vitro analyses related to skeletal muscle. Here we present data regarding the autophagic response induced by two chemicals known to influence calcium release and contraction in skeletal muscles and C2C12 cells: acetylcholine and caffeine. More specifically, by concurrently administering acetylcholine or caffeine along with chloroquine to differentiated myotubes for various amounts of time and assessing the protein expression of LC3 and p62, we report data on the relative level of autophagic flux induced by these two calcium- and contraction-regulating chemicals.
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spelling pubmed-48025402016-04-06 Autophagic flux data in differentiated C2C12 myotubes following exposure to acetylcholine and caffeine Bloemberg, Darin Quadrilatero, Joe Data Brief Data Article The C2C12 line of mouse myoblasts is a useful cell culture model in which to conduct in vitro analyses related to skeletal muscle. Here we present data regarding the autophagic response induced by two chemicals known to influence calcium release and contraction in skeletal muscles and C2C12 cells: acetylcholine and caffeine. More specifically, by concurrently administering acetylcholine or caffeine along with chloroquine to differentiated myotubes for various amounts of time and assessing the protein expression of LC3 and p62, we report data on the relative level of autophagic flux induced by these two calcium- and contraction-regulating chemicals. Elsevier 2016-03-10 /pmc/articles/PMC4802540/ /pubmed/27054179 http://dx.doi.org/10.1016/j.dib.2016.03.008 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Bloemberg, Darin
Quadrilatero, Joe
Autophagic flux data in differentiated C2C12 myotubes following exposure to acetylcholine and caffeine
title Autophagic flux data in differentiated C2C12 myotubes following exposure to acetylcholine and caffeine
title_full Autophagic flux data in differentiated C2C12 myotubes following exposure to acetylcholine and caffeine
title_fullStr Autophagic flux data in differentiated C2C12 myotubes following exposure to acetylcholine and caffeine
title_full_unstemmed Autophagic flux data in differentiated C2C12 myotubes following exposure to acetylcholine and caffeine
title_short Autophagic flux data in differentiated C2C12 myotubes following exposure to acetylcholine and caffeine
title_sort autophagic flux data in differentiated c2c12 myotubes following exposure to acetylcholine and caffeine
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802540/
https://www.ncbi.nlm.nih.gov/pubmed/27054179
http://dx.doi.org/10.1016/j.dib.2016.03.008
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