The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells

Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, “Development of a cell-defined siRNA microarray for analysis of gene functionin hum...

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Autores principales: Kim, Hi Chul, Kim, Gi-Hwan, Shum, David, Cho, Ssang-Goo, Lee, Eun Ju, Kwon, Yong-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802669/
https://www.ncbi.nlm.nih.gov/pubmed/27054175
http://dx.doi.org/10.1016/j.dib.2016.03.040
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author Kim, Hi Chul
Kim, Gi-Hwan
Shum, David
Cho, Ssang-Goo
Lee, Eun Ju
Kwon, Yong-Jun
author_facet Kim, Hi Chul
Kim, Gi-Hwan
Shum, David
Cho, Ssang-Goo
Lee, Eun Ju
Kwon, Yong-Jun
author_sort Kim, Hi Chul
collection PubMed
description Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, “Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells” (Kim et al., 2016 [1]). First, we confirmed that p65 protein inhibition efficiency was maintained when hBMSCs were culture for 7 days on the siRNA spot, and siRNA spot activity remained in spite of long term storage (10 days and 2 months). Additionally, we confirmed p65 protein inhibition in U2OS cells after 48 h reverse transfection.
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spelling pubmed-48026692016-04-06 The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells Kim, Hi Chul Kim, Gi-Hwan Shum, David Cho, Ssang-Goo Lee, Eun Ju Kwon, Yong-Jun Data Brief Data Article Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, “Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells” (Kim et al., 2016 [1]). First, we confirmed that p65 protein inhibition efficiency was maintained when hBMSCs were culture for 7 days on the siRNA spot, and siRNA spot activity remained in spite of long term storage (10 days and 2 months). Additionally, we confirmed p65 protein inhibition in U2OS cells after 48 h reverse transfection. Elsevier 2016-03-12 /pmc/articles/PMC4802669/ /pubmed/27054175 http://dx.doi.org/10.1016/j.dib.2016.03.040 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Kim, Hi Chul
Kim, Gi-Hwan
Shum, David
Cho, Ssang-Goo
Lee, Eun Ju
Kwon, Yong-Jun
The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
title The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
title_full The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
title_fullStr The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
title_full_unstemmed The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
title_short The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
title_sort monitoring of gene functions on a cell-defined sirna microarray in human bone marrow stromal and u2os cells
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802669/
https://www.ncbi.nlm.nih.gov/pubmed/27054175
http://dx.doi.org/10.1016/j.dib.2016.03.040
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