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Data supporting the involvement of the adenine nucleotide translocase conformation in opening the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria
There we made available information about the effects of the adenine nucleotide translocase (ANT) ‘c’ conformation fixers (phenylarsine oxide (PAO), tert-butylhydroperoxide (tBHP), and carboxyatractyloside) as well as thiol reagent (4,4′-diisothiocyanostilbene-2,2′-disulfonate (DIDS)) on isolated ra...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Elsevier
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802814/ https://www.ncbi.nlm.nih.gov/pubmed/27054168 http://dx.doi.org/10.1016/j.dib.2016.03.030 |
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author | Korotkov, Sergey M. |
author_facet | Korotkov, Sergey M. |
author_sort | Korotkov, Sergey M. |
collection | PubMed |
description | There we made available information about the effects of the adenine nucleotide translocase (ANT) ‘c’ conformation fixers (phenylarsine oxide (PAO), tert-butylhydroperoxide (tBHP), and carboxyatractyloside) as well as thiol reagent (4,4′-diisothiocyanostilbene-2,2′-disulfonate (DIDS)) on isolated rat liver mitochondria. We observed a decrease in A(540) (mitochondrial swelling) and respiratory control rates (RCR(ADP) [state 3/state 4] and RCR(DNP) [2,4-dinitrophenol-uncoupled state/basal state or state 4]), as well as an increase in Ca(2+)-induced safranin fluorescence (F(485/590), arbitrary units), showed a dissipation in the inner membrane potential (ΔΨ(mito)), in experiments with energized rat liver mitochondria, injected into the buffer containing 25–75 mM TlNO(3), 125 mM KNO(3), and 100 µM Ca(2+). The fixers and DIDS, in comparison to Ca(2+) alone, greatly increased A(540) decline and the rate of Ca(2+)-induced ΔΨ(mito) dissipation. These reagents also markedly decreased RCR(ADP) and RCR(DNP). The MPTP inhibitors (ADP, cyclosporin A, bongkrekic acid, and N-ethylmaleimide) fixing the ANT in ‘m’ conformation significantly hindered the above-mentioned effects of the fixers and DIDS. A more complete scientific analysis of these findings may be obtained from the manuscript “To involvement the conformation of the adenine nucleotide translocase in opening the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria” (Korotkov et al., 2016 [1]). |
format | Online Article Text |
id | pubmed-4802814 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-48028142016-04-06 Data supporting the involvement of the adenine nucleotide translocase conformation in opening the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria Korotkov, Sergey M. Data Brief Data Article There we made available information about the effects of the adenine nucleotide translocase (ANT) ‘c’ conformation fixers (phenylarsine oxide (PAO), tert-butylhydroperoxide (tBHP), and carboxyatractyloside) as well as thiol reagent (4,4′-diisothiocyanostilbene-2,2′-disulfonate (DIDS)) on isolated rat liver mitochondria. We observed a decrease in A(540) (mitochondrial swelling) and respiratory control rates (RCR(ADP) [state 3/state 4] and RCR(DNP) [2,4-dinitrophenol-uncoupled state/basal state or state 4]), as well as an increase in Ca(2+)-induced safranin fluorescence (F(485/590), arbitrary units), showed a dissipation in the inner membrane potential (ΔΨ(mito)), in experiments with energized rat liver mitochondria, injected into the buffer containing 25–75 mM TlNO(3), 125 mM KNO(3), and 100 µM Ca(2+). The fixers and DIDS, in comparison to Ca(2+) alone, greatly increased A(540) decline and the rate of Ca(2+)-induced ΔΨ(mito) dissipation. These reagents also markedly decreased RCR(ADP) and RCR(DNP). The MPTP inhibitors (ADP, cyclosporin A, bongkrekic acid, and N-ethylmaleimide) fixing the ANT in ‘m’ conformation significantly hindered the above-mentioned effects of the fixers and DIDS. A more complete scientific analysis of these findings may be obtained from the manuscript “To involvement the conformation of the adenine nucleotide translocase in opening the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria” (Korotkov et al., 2016 [1]). Elsevier 2016-03-11 /pmc/articles/PMC4802814/ /pubmed/27054168 http://dx.doi.org/10.1016/j.dib.2016.03.030 Text en © 2016 The Author http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Korotkov, Sergey M. Data supporting the involvement of the adenine nucleotide translocase conformation in opening the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria |
title | Data supporting the involvement of the adenine nucleotide translocase conformation in opening the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria |
title_full | Data supporting the involvement of the adenine nucleotide translocase conformation in opening the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria |
title_fullStr | Data supporting the involvement of the adenine nucleotide translocase conformation in opening the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria |
title_full_unstemmed | Data supporting the involvement of the adenine nucleotide translocase conformation in opening the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria |
title_short | Data supporting the involvement of the adenine nucleotide translocase conformation in opening the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria |
title_sort | data supporting the involvement of the adenine nucleotide translocase conformation in opening the tl(+)-induced permeability transition pore in ca(2+)-loaded rat liver mitochondria |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802814/ https://www.ncbi.nlm.nih.gov/pubmed/27054168 http://dx.doi.org/10.1016/j.dib.2016.03.030 |
work_keys_str_mv | AT korotkovsergeym datasupportingtheinvolvementoftheadeninenucleotidetranslocaseconformationinopeningthetlinducedpermeabilitytransitionporeinca2loadedratlivermitochondria |