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Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock

In vertebrate development, the sequential and rhythmic segmentation of the body axis is regulated by a “segmentation clock”. This clock is comprised of a population of coordinated oscillating cells that together produce rhythmic gene expression patterns in the embryo. Whether individual cells autono...

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Autores principales: Webb, Alexis B, Lengyel, Iván M, Jörg, David J, Valentin, Guillaume, Jülicher, Frank, Morelli, Luis G, Oates, Andrew C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803185/
https://www.ncbi.nlm.nih.gov/pubmed/26880542
http://dx.doi.org/10.7554/eLife.08438
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author Webb, Alexis B
Lengyel, Iván M
Jörg, David J
Valentin, Guillaume
Jülicher, Frank
Morelli, Luis G
Oates, Andrew C
author_facet Webb, Alexis B
Lengyel, Iván M
Jörg, David J
Valentin, Guillaume
Jülicher, Frank
Morelli, Luis G
Oates, Andrew C
author_sort Webb, Alexis B
collection PubMed
description In vertebrate development, the sequential and rhythmic segmentation of the body axis is regulated by a “segmentation clock”. This clock is comprised of a population of coordinated oscillating cells that together produce rhythmic gene expression patterns in the embryo. Whether individual cells autonomously maintain oscillations, or whether oscillations depend on signals from neighboring cells is unknown. Using a transgenic zebrafish reporter line for the cyclic transcription factor Her1, we recorded single tailbud cells in vitro. We demonstrate that individual cells can behave as autonomous cellular oscillators. We described the observed variability in cell behavior using a theory of generic oscillators with correlated noise. Single cells have longer periods and lower precision than the tissue, highlighting the role of collective processes in the segmentation clock. Our work reveals a population of cells from the zebrafish segmentation clock that behave as self-sustained, autonomous oscillators with distinctive noisy dynamics. DOI: http://dx.doi.org/10.7554/eLife.08438.001
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spelling pubmed-48031852016-03-24 Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock Webb, Alexis B Lengyel, Iván M Jörg, David J Valentin, Guillaume Jülicher, Frank Morelli, Luis G Oates, Andrew C eLife Computational and Systems Biology In vertebrate development, the sequential and rhythmic segmentation of the body axis is regulated by a “segmentation clock”. This clock is comprised of a population of coordinated oscillating cells that together produce rhythmic gene expression patterns in the embryo. Whether individual cells autonomously maintain oscillations, or whether oscillations depend on signals from neighboring cells is unknown. Using a transgenic zebrafish reporter line for the cyclic transcription factor Her1, we recorded single tailbud cells in vitro. We demonstrate that individual cells can behave as autonomous cellular oscillators. We described the observed variability in cell behavior using a theory of generic oscillators with correlated noise. Single cells have longer periods and lower precision than the tissue, highlighting the role of collective processes in the segmentation clock. Our work reveals a population of cells from the zebrafish segmentation clock that behave as self-sustained, autonomous oscillators with distinctive noisy dynamics. DOI: http://dx.doi.org/10.7554/eLife.08438.001 eLife Sciences Publications, Ltd 2016-02-13 /pmc/articles/PMC4803185/ /pubmed/26880542 http://dx.doi.org/10.7554/eLife.08438 Text en © 2016, Webb et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Computational and Systems Biology
Webb, Alexis B
Lengyel, Iván M
Jörg, David J
Valentin, Guillaume
Jülicher, Frank
Morelli, Luis G
Oates, Andrew C
Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock
title Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock
title_full Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock
title_fullStr Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock
title_full_unstemmed Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock
title_short Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock
title_sort persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock
topic Computational and Systems Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803185/
https://www.ncbi.nlm.nih.gov/pubmed/26880542
http://dx.doi.org/10.7554/eLife.08438
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