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Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility
Geminin controls proper centrosome duplication, cell division, and differentiation. We investigated the function of geminin in oogenesis, fertilization, and early embryo development by deleting the geminin gene in oocytes from the primordial follicle stage. Oocyte-specific disruption of geminin resu...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803303/ https://www.ncbi.nlm.nih.gov/pubmed/26764091 http://dx.doi.org/10.1091/mbc.E15-06-0346 |
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author | Ma, Xue-Shan Lin, Fei Wang, Zhong-Wei Hu, Meng-Wen Huang, Lin Meng, Tie-Gang Jiang, Zong-Zhe Schatten, Heide Wang, Zhen-Bo Sun, Qing-Yuan |
author_facet | Ma, Xue-Shan Lin, Fei Wang, Zhong-Wei Hu, Meng-Wen Huang, Lin Meng, Tie-Gang Jiang, Zong-Zhe Schatten, Heide Wang, Zhen-Bo Sun, Qing-Yuan |
author_sort | Ma, Xue-Shan |
collection | PubMed |
description | Geminin controls proper centrosome duplication, cell division, and differentiation. We investigated the function of geminin in oogenesis, fertilization, and early embryo development by deleting the geminin gene in oocytes from the primordial follicle stage. Oocyte-specific disruption of geminin results in low fertility in mice. Even though there was no evident anomaly of oogenesis, oocyte meiotic maturation, natural ovulation, or fertilization, early embryo development and implantation were impaired. The fertilized eggs derived from mutant mice showed developmental delay, and many were blocked at the late zygote stage. Cdt1 protein was decreased, whereas Chk1 and H2AX phosphorylation was increased, in fertilized eggs after geminin depletion. Our results suggest that disruption of maternal geminin may decrease Cdt1 expression and cause DNA rereplication, which then activates the cell cycle checkpoint and DNA damage repair and thus impairs early embryo development. |
format | Online Article Text |
id | pubmed-4803303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-48033032016-05-16 Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility Ma, Xue-Shan Lin, Fei Wang, Zhong-Wei Hu, Meng-Wen Huang, Lin Meng, Tie-Gang Jiang, Zong-Zhe Schatten, Heide Wang, Zhen-Bo Sun, Qing-Yuan Mol Biol Cell Articles Geminin controls proper centrosome duplication, cell division, and differentiation. We investigated the function of geminin in oogenesis, fertilization, and early embryo development by deleting the geminin gene in oocytes from the primordial follicle stage. Oocyte-specific disruption of geminin results in low fertility in mice. Even though there was no evident anomaly of oogenesis, oocyte meiotic maturation, natural ovulation, or fertilization, early embryo development and implantation were impaired. The fertilized eggs derived from mutant mice showed developmental delay, and many were blocked at the late zygote stage. Cdt1 protein was decreased, whereas Chk1 and H2AX phosphorylation was increased, in fertilized eggs after geminin depletion. Our results suggest that disruption of maternal geminin may decrease Cdt1 expression and cause DNA rereplication, which then activates the cell cycle checkpoint and DNA damage repair and thus impairs early embryo development. The American Society for Cell Biology 2016-03-01 /pmc/articles/PMC4803303/ /pubmed/26764091 http://dx.doi.org/10.1091/mbc.E15-06-0346 Text en © 2016 Ma et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Ma, Xue-Shan Lin, Fei Wang, Zhong-Wei Hu, Meng-Wen Huang, Lin Meng, Tie-Gang Jiang, Zong-Zhe Schatten, Heide Wang, Zhen-Bo Sun, Qing-Yuan Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility |
title | Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility |
title_full | Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility |
title_fullStr | Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility |
title_full_unstemmed | Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility |
title_short | Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility |
title_sort | geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803303/ https://www.ncbi.nlm.nih.gov/pubmed/26764091 http://dx.doi.org/10.1091/mbc.E15-06-0346 |
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