Cargando…

Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility

Geminin controls proper centrosome duplication, cell division, and differentiation. We investigated the function of geminin in oogenesis, fertilization, and early embryo development by deleting the geminin gene in oocytes from the primordial follicle stage. Oocyte-specific disruption of geminin resu...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Xue-Shan, Lin, Fei, Wang, Zhong-Wei, Hu, Meng-Wen, Huang, Lin, Meng, Tie-Gang, Jiang, Zong-Zhe, Schatten, Heide, Wang, Zhen-Bo, Sun, Qing-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803303/
https://www.ncbi.nlm.nih.gov/pubmed/26764091
http://dx.doi.org/10.1091/mbc.E15-06-0346
_version_ 1782422862592737280
author Ma, Xue-Shan
Lin, Fei
Wang, Zhong-Wei
Hu, Meng-Wen
Huang, Lin
Meng, Tie-Gang
Jiang, Zong-Zhe
Schatten, Heide
Wang, Zhen-Bo
Sun, Qing-Yuan
author_facet Ma, Xue-Shan
Lin, Fei
Wang, Zhong-Wei
Hu, Meng-Wen
Huang, Lin
Meng, Tie-Gang
Jiang, Zong-Zhe
Schatten, Heide
Wang, Zhen-Bo
Sun, Qing-Yuan
author_sort Ma, Xue-Shan
collection PubMed
description Geminin controls proper centrosome duplication, cell division, and differentiation. We investigated the function of geminin in oogenesis, fertilization, and early embryo development by deleting the geminin gene in oocytes from the primordial follicle stage. Oocyte-specific disruption of geminin results in low fertility in mice. Even though there was no evident anomaly of oogenesis, oocyte meiotic maturation, natural ovulation, or fertilization, early embryo development and implantation were impaired. The fertilized eggs derived from mutant mice showed developmental delay, and many were blocked at the late zygote stage. Cdt1 protein was decreased, whereas Chk1 and H2AX phosphorylation was increased, in fertilized eggs after geminin depletion. Our results suggest that disruption of maternal geminin may decrease Cdt1 expression and cause DNA rereplication, which then activates the cell cycle checkpoint and DNA damage repair and thus impairs early embryo development.
format Online
Article
Text
id pubmed-4803303
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-48033032016-05-16 Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility Ma, Xue-Shan Lin, Fei Wang, Zhong-Wei Hu, Meng-Wen Huang, Lin Meng, Tie-Gang Jiang, Zong-Zhe Schatten, Heide Wang, Zhen-Bo Sun, Qing-Yuan Mol Biol Cell Articles Geminin controls proper centrosome duplication, cell division, and differentiation. We investigated the function of geminin in oogenesis, fertilization, and early embryo development by deleting the geminin gene in oocytes from the primordial follicle stage. Oocyte-specific disruption of geminin results in low fertility in mice. Even though there was no evident anomaly of oogenesis, oocyte meiotic maturation, natural ovulation, or fertilization, early embryo development and implantation were impaired. The fertilized eggs derived from mutant mice showed developmental delay, and many were blocked at the late zygote stage. Cdt1 protein was decreased, whereas Chk1 and H2AX phosphorylation was increased, in fertilized eggs after geminin depletion. Our results suggest that disruption of maternal geminin may decrease Cdt1 expression and cause DNA rereplication, which then activates the cell cycle checkpoint and DNA damage repair and thus impairs early embryo development. The American Society for Cell Biology 2016-03-01 /pmc/articles/PMC4803303/ /pubmed/26764091 http://dx.doi.org/10.1091/mbc.E15-06-0346 Text en © 2016 Ma et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Ma, Xue-Shan
Lin, Fei
Wang, Zhong-Wei
Hu, Meng-Wen
Huang, Lin
Meng, Tie-Gang
Jiang, Zong-Zhe
Schatten, Heide
Wang, Zhen-Bo
Sun, Qing-Yuan
Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility
title Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility
title_full Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility
title_fullStr Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility
title_full_unstemmed Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility
title_short Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility
title_sort geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803303/
https://www.ncbi.nlm.nih.gov/pubmed/26764091
http://dx.doi.org/10.1091/mbc.E15-06-0346
work_keys_str_mv AT maxueshan geminindeletioninmouseoocytesresultsinimpairedembryodevelopmentandreducedfertility
AT linfei geminindeletioninmouseoocytesresultsinimpairedembryodevelopmentandreducedfertility
AT wangzhongwei geminindeletioninmouseoocytesresultsinimpairedembryodevelopmentandreducedfertility
AT humengwen geminindeletioninmouseoocytesresultsinimpairedembryodevelopmentandreducedfertility
AT huanglin geminindeletioninmouseoocytesresultsinimpairedembryodevelopmentandreducedfertility
AT mengtiegang geminindeletioninmouseoocytesresultsinimpairedembryodevelopmentandreducedfertility
AT jiangzongzhe geminindeletioninmouseoocytesresultsinimpairedembryodevelopmentandreducedfertility
AT schattenheide geminindeletioninmouseoocytesresultsinimpairedembryodevelopmentandreducedfertility
AT wangzhenbo geminindeletioninmouseoocytesresultsinimpairedembryodevelopmentandreducedfertility
AT sunqingyuan geminindeletioninmouseoocytesresultsinimpairedembryodevelopmentandreducedfertility