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Low Prevalence of Somatic TERT Promoter Mutations in Classic Papillary Thyroid Carcinoma
BACKGROUND: Transcriptional activating mutations of telomerase reverse transcriptase (TERT) are associated with more aggressive thyroid cancer. We evaluated the significance of TERT promoter mutations in Korean patients with classic papillary thyroid cancer (PTC). METHODS: Genomic DNA was isolated f...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Endocrine Society
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803544/ https://www.ncbi.nlm.nih.gov/pubmed/26676331 http://dx.doi.org/10.3803/EnM.2016.31.1.100 |
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author | Jeon, Min Ji Kim, Won Gu Sim, Soyoung Lim, Seonhee Kwon, Hyemi Kim, Tae Yong Shong, Young Kee Kim, Won Bae |
author_facet | Jeon, Min Ji Kim, Won Gu Sim, Soyoung Lim, Seonhee Kwon, Hyemi Kim, Tae Yong Shong, Young Kee Kim, Won Bae |
author_sort | Jeon, Min Ji |
collection | PubMed |
description | BACKGROUND: Transcriptional activating mutations of telomerase reverse transcriptase (TERT) are associated with more aggressive thyroid cancer. We evaluated the significance of TERT promoter mutations in Korean patients with classic papillary thyroid cancer (PTC). METHODS: Genomic DNA was isolated from four thyroid cancer cell lines and 35 fresh-frozen PTC tissues. TERT promoter mutations (C228T and C250T) and the BRAF V600E mutation were evaluated by polymerase chain reaction amplification and direct sequencing. RESULTS: The CC228229TT mutation in the TERT promoter was detected in BCPAP cells and the C250T mutation was found in 8505C cells. No TERT promoter mutation was observed in Cal-62 or ML-1 cells. The C228T mutation was found in only 1 of 35 (2.8%) PTCs and no C250T mutations were detected in any of the study subjects. The BRAF V600E mutation was found in 20 of 35 (57.1%) PTCs. One patient with the C228T TERT mutation also harbored the BRAF V600E mutation and developed a recurrence. CONCLUSION: The prevalence of somatic TERT promoter mutations was low in Korean patients with classic PTC. Therefore, the prognostic role of TERT promoter mutations might be limited in this patient cohort. |
format | Online Article Text |
id | pubmed-4803544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Korean Endocrine Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-48035442016-03-27 Low Prevalence of Somatic TERT Promoter Mutations in Classic Papillary Thyroid Carcinoma Jeon, Min Ji Kim, Won Gu Sim, Soyoung Lim, Seonhee Kwon, Hyemi Kim, Tae Yong Shong, Young Kee Kim, Won Bae Endocrinol Metab (Seoul) Original Article BACKGROUND: Transcriptional activating mutations of telomerase reverse transcriptase (TERT) are associated with more aggressive thyroid cancer. We evaluated the significance of TERT promoter mutations in Korean patients with classic papillary thyroid cancer (PTC). METHODS: Genomic DNA was isolated from four thyroid cancer cell lines and 35 fresh-frozen PTC tissues. TERT promoter mutations (C228T and C250T) and the BRAF V600E mutation were evaluated by polymerase chain reaction amplification and direct sequencing. RESULTS: The CC228229TT mutation in the TERT promoter was detected in BCPAP cells and the C250T mutation was found in 8505C cells. No TERT promoter mutation was observed in Cal-62 or ML-1 cells. The C228T mutation was found in only 1 of 35 (2.8%) PTCs and no C250T mutations were detected in any of the study subjects. The BRAF V600E mutation was found in 20 of 35 (57.1%) PTCs. One patient with the C228T TERT mutation also harbored the BRAF V600E mutation and developed a recurrence. CONCLUSION: The prevalence of somatic TERT promoter mutations was low in Korean patients with classic PTC. Therefore, the prognostic role of TERT promoter mutations might be limited in this patient cohort. Korean Endocrine Society 2016-03 2016-03-16 /pmc/articles/PMC4803544/ /pubmed/26676331 http://dx.doi.org/10.3803/EnM.2016.31.1.100 Text en Copyright © 2016 Korean Endocrine Society http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Jeon, Min Ji Kim, Won Gu Sim, Soyoung Lim, Seonhee Kwon, Hyemi Kim, Tae Yong Shong, Young Kee Kim, Won Bae Low Prevalence of Somatic TERT Promoter Mutations in Classic Papillary Thyroid Carcinoma |
title | Low Prevalence of Somatic TERT Promoter Mutations in Classic Papillary Thyroid Carcinoma |
title_full | Low Prevalence of Somatic TERT Promoter Mutations in Classic Papillary Thyroid Carcinoma |
title_fullStr | Low Prevalence of Somatic TERT Promoter Mutations in Classic Papillary Thyroid Carcinoma |
title_full_unstemmed | Low Prevalence of Somatic TERT Promoter Mutations in Classic Papillary Thyroid Carcinoma |
title_short | Low Prevalence of Somatic TERT Promoter Mutations in Classic Papillary Thyroid Carcinoma |
title_sort | low prevalence of somatic tert promoter mutations in classic papillary thyroid carcinoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803544/ https://www.ncbi.nlm.nih.gov/pubmed/26676331 http://dx.doi.org/10.3803/EnM.2016.31.1.100 |
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