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Culture and Real-Time PCR Based Maternal Screening and Antibiotic Susceptibility for Group B Streptococcus: An Iranian Experience
INTRODUCTION: Vertical Transmission of group B streptococcus (GBS) from a vagina colonized mother to her infant upon rupture of membranes (ROM) or after the onset of labor can cause life-threatening infections in newborn. Although intrapartum antibiotic prophylaxis (IAP) can significantly decrease n...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Canadian Center of Science and Education
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803879/ https://www.ncbi.nlm.nih.gov/pubmed/26153188 http://dx.doi.org/10.5539/gjhs.v7n6p233 |
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author | Goudarzi, Gholamreza Ghafarzadeh, Masoumeh Shakib, Pegah Anbari, Khatereh |
author_facet | Goudarzi, Gholamreza Ghafarzadeh, Masoumeh Shakib, Pegah Anbari, Khatereh |
author_sort | Goudarzi, Gholamreza |
collection | PubMed |
description | INTRODUCTION: Vertical Transmission of group B streptococcus (GBS) from a vagina colonized mother to her infant upon rupture of membranes (ROM) or after the onset of labor can cause life-threatening infections in newborn. Although intrapartum antibiotic prophylaxis (IAP) can significantly decrease neonatal GBS diseases, this issue has potentiated the emergence of antibiotic resistance strains. Our study examined the colonization rate of GBS using real-time PCR and culture methods, and trends in antibiotic resistance of GBS isolates obtained from pregnant women in Khorramabad, Iran. METHODS: In this cross-sectional study, two vaginal-rectal swabs were collected and analyzed separately from 100 pregnant women at 35-37 weeks of gestation by convenience sampling method. The specimens were subjected to GBS detection using real-time PCR assay and standard culture. Susceptibility pattern of the GBS isolates was examined using the disk diffusion method. RESULTS: GBS carriage rate was 17% and 19% using culture and real-time PCR, respectively. In six samples, the culture was positive and the real-time PCR was negative. Sensitivity and specificity for real-time PCR were 72.7% and 96.1%, respectively using culture as the gold standard. Amongst twenty-two isolates examined, 100% resistance to erythromycin and clindamycin was observed. One isolate (4%) exhibited resistance to penicillin. CONCLUSION: Considering the relatively high GBS carriage rate in Khorramabad, routine antepartum screening for GBS is recommended. Penicillin can remain the antibiotic of choice for IAP; however, in penicillin-allergic mothers, vancomycin can be an alternative antibiotic. |
format | Online Article Text |
id | pubmed-4803879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Canadian Center of Science and Education |
record_format | MEDLINE/PubMed |
spelling | pubmed-48038792016-04-21 Culture and Real-Time PCR Based Maternal Screening and Antibiotic Susceptibility for Group B Streptococcus: An Iranian Experience Goudarzi, Gholamreza Ghafarzadeh, Masoumeh Shakib, Pegah Anbari, Khatereh Glob J Health Sci Articles INTRODUCTION: Vertical Transmission of group B streptococcus (GBS) from a vagina colonized mother to her infant upon rupture of membranes (ROM) or after the onset of labor can cause life-threatening infections in newborn. Although intrapartum antibiotic prophylaxis (IAP) can significantly decrease neonatal GBS diseases, this issue has potentiated the emergence of antibiotic resistance strains. Our study examined the colonization rate of GBS using real-time PCR and culture methods, and trends in antibiotic resistance of GBS isolates obtained from pregnant women in Khorramabad, Iran. METHODS: In this cross-sectional study, two vaginal-rectal swabs were collected and analyzed separately from 100 pregnant women at 35-37 weeks of gestation by convenience sampling method. The specimens were subjected to GBS detection using real-time PCR assay and standard culture. Susceptibility pattern of the GBS isolates was examined using the disk diffusion method. RESULTS: GBS carriage rate was 17% and 19% using culture and real-time PCR, respectively. In six samples, the culture was positive and the real-time PCR was negative. Sensitivity and specificity for real-time PCR were 72.7% and 96.1%, respectively using culture as the gold standard. Amongst twenty-two isolates examined, 100% resistance to erythromycin and clindamycin was observed. One isolate (4%) exhibited resistance to penicillin. CONCLUSION: Considering the relatively high GBS carriage rate in Khorramabad, routine antepartum screening for GBS is recommended. Penicillin can remain the antibiotic of choice for IAP; however, in penicillin-allergic mothers, vancomycin can be an alternative antibiotic. Canadian Center of Science and Education 2015-11 2015-04-16 /pmc/articles/PMC4803879/ /pubmed/26153188 http://dx.doi.org/10.5539/gjhs.v7n6p233 Text en Copyright: © Canadian Center of Science and Education http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Articles Goudarzi, Gholamreza Ghafarzadeh, Masoumeh Shakib, Pegah Anbari, Khatereh Culture and Real-Time PCR Based Maternal Screening and Antibiotic Susceptibility for Group B Streptococcus: An Iranian Experience |
title | Culture and Real-Time PCR Based Maternal Screening and Antibiotic Susceptibility for Group B Streptococcus: An Iranian Experience |
title_full | Culture and Real-Time PCR Based Maternal Screening and Antibiotic Susceptibility for Group B Streptococcus: An Iranian Experience |
title_fullStr | Culture and Real-Time PCR Based Maternal Screening and Antibiotic Susceptibility for Group B Streptococcus: An Iranian Experience |
title_full_unstemmed | Culture and Real-Time PCR Based Maternal Screening and Antibiotic Susceptibility for Group B Streptococcus: An Iranian Experience |
title_short | Culture and Real-Time PCR Based Maternal Screening and Antibiotic Susceptibility for Group B Streptococcus: An Iranian Experience |
title_sort | culture and real-time pcr based maternal screening and antibiotic susceptibility for group b streptococcus: an iranian experience |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803879/ https://www.ncbi.nlm.nih.gov/pubmed/26153188 http://dx.doi.org/10.5539/gjhs.v7n6p233 |
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