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Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique

Many pathologies of skin, especially ageing and cancer, involve modifications in the matrix alignment. Such tissue reorganization could have impact on cell behaviour and/or more global biological processes. Tissue engineering provides accurate study model by mimicking the skin and it allows the cons...

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Detalles Bibliográficos
Autores principales: Chabaud, Stéphane, Bolduc, Stéphane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804048/
https://www.ncbi.nlm.nih.gov/pubmed/27051415
http://dx.doi.org/10.1155/2016/5397319
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author Chabaud, Stéphane
Bolduc, Stéphane
author_facet Chabaud, Stéphane
Bolduc, Stéphane
author_sort Chabaud, Stéphane
collection PubMed
description Many pathologies of skin, especially ageing and cancer, involve modifications in the matrix alignment. Such tissue reorganization could have impact on cell behaviour and/or more global biological processes. Tissue engineering provides accurate study model by mimicking the skin and it allows the construction of versatile tridimensional models using human cells. It also avoids the use of animals, which gave sometimes nontranslatable results. Among the various techniques existing, the self-assembly method allows production of a near native skin, free of exogenous material. After cultivating human dermal fibroblasts in the presence of ascorbate during two weeks, a reseeding of these cells takes place after elevation of the resulting stroma on a permeable ring and culture pursued for another two weeks. This protocol induces a clear realignment of matrix fibres and cells parallel to the horizon. The thickness of this stretched reconstructed tissue is reduced compared to the stroma produced by the standard technique. Cell count is also reduced. In conclusion, a new, easy, and inexpensive method to produce aligned tissue free of exogenous material could be used for fundamental research applications in dermatology.
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spelling pubmed-48040482016-04-05 Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique Chabaud, Stéphane Bolduc, Stéphane Dermatol Res Pract Research Article Many pathologies of skin, especially ageing and cancer, involve modifications in the matrix alignment. Such tissue reorganization could have impact on cell behaviour and/or more global biological processes. Tissue engineering provides accurate study model by mimicking the skin and it allows the construction of versatile tridimensional models using human cells. It also avoids the use of animals, which gave sometimes nontranslatable results. Among the various techniques existing, the self-assembly method allows production of a near native skin, free of exogenous material. After cultivating human dermal fibroblasts in the presence of ascorbate during two weeks, a reseeding of these cells takes place after elevation of the resulting stroma on a permeable ring and culture pursued for another two weeks. This protocol induces a clear realignment of matrix fibres and cells parallel to the horizon. The thickness of this stretched reconstructed tissue is reduced compared to the stroma produced by the standard technique. Cell count is also reduced. In conclusion, a new, easy, and inexpensive method to produce aligned tissue free of exogenous material could be used for fundamental research applications in dermatology. Hindawi Publishing Corporation 2016 2016-03-09 /pmc/articles/PMC4804048/ /pubmed/27051415 http://dx.doi.org/10.1155/2016/5397319 Text en Copyright © 2016 S. Chabaud and S. Bolduc. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chabaud, Stéphane
Bolduc, Stéphane
Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique
title Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique
title_full Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique
title_fullStr Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique
title_full_unstemmed Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique
title_short Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique
title_sort production of a self-aligned scaffold, free of exogenous material, from dermal fibroblasts using the self-assembly technique
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804048/
https://www.ncbi.nlm.nih.gov/pubmed/27051415
http://dx.doi.org/10.1155/2016/5397319
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