Cloning of chrysanthemum high-affinity nitrate transporter family (CmNRT2) and characterization of CmNRT2.1

The family of NITRATE TRANSPORTER 2 (NRT2) proteins belongs to the high affinity transport system (HATS) proteins which acts at low nitrate concentrations. The relevant gene content of the chrysanthemum genome was explored here by isolating the full length sequences of six distinct CmNRT2 genes. One...

Descripción completa

Detalles Bibliográficos
Autores principales: Gu, Chunsun, Song, Aiping, Zhang, Xiaoxue, Wang, Haibin, Li, Ting, Chen, Yu, Jiang, Jiafu, Chen, Fadi, Chen, Sumei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804277/
https://www.ncbi.nlm.nih.gov/pubmed/27004464
http://dx.doi.org/10.1038/srep23462
_version_ 1782422993126817792
author Gu, Chunsun
Song, Aiping
Zhang, Xiaoxue
Wang, Haibin
Li, Ting
Chen, Yu
Jiang, Jiafu
Chen, Fadi
Chen, Sumei
author_facet Gu, Chunsun
Song, Aiping
Zhang, Xiaoxue
Wang, Haibin
Li, Ting
Chen, Yu
Jiang, Jiafu
Chen, Fadi
Chen, Sumei
author_sort Gu, Chunsun
collection PubMed
description The family of NITRATE TRANSPORTER 2 (NRT2) proteins belongs to the high affinity transport system (HATS) proteins which acts at low nitrate concentrations. The relevant gene content of the chrysanthemum genome was explored here by isolating the full length sequences of six distinct CmNRT2 genes. One of these (CmNRT2.1) was investigated at the functional level. Its transcription level was inducible by low concentrations of both nitrate and ammonium. A yeast two hybrid assay showed that CmNRT2.1 interacts with CmNAR2, while a BiFC assay demonstrated that the interaction occurs at the plasma membrane. Arabidopsis thaliana plants heterologously expressing CmNRT2.1 displayed an enhanced rate of labeled nitrogen uptake, suggesting that CmNRT2.1 represents a high affinity root nitrate transporter.
format Online
Article
Text
id pubmed-4804277
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-48042772016-03-23 Cloning of chrysanthemum high-affinity nitrate transporter family (CmNRT2) and characterization of CmNRT2.1 Gu, Chunsun Song, Aiping Zhang, Xiaoxue Wang, Haibin Li, Ting Chen, Yu Jiang, Jiafu Chen, Fadi Chen, Sumei Sci Rep Article The family of NITRATE TRANSPORTER 2 (NRT2) proteins belongs to the high affinity transport system (HATS) proteins which acts at low nitrate concentrations. The relevant gene content of the chrysanthemum genome was explored here by isolating the full length sequences of six distinct CmNRT2 genes. One of these (CmNRT2.1) was investigated at the functional level. Its transcription level was inducible by low concentrations of both nitrate and ammonium. A yeast two hybrid assay showed that CmNRT2.1 interacts with CmNAR2, while a BiFC assay demonstrated that the interaction occurs at the plasma membrane. Arabidopsis thaliana plants heterologously expressing CmNRT2.1 displayed an enhanced rate of labeled nitrogen uptake, suggesting that CmNRT2.1 represents a high affinity root nitrate transporter. Nature Publishing Group 2016-03-23 /pmc/articles/PMC4804277/ /pubmed/27004464 http://dx.doi.org/10.1038/srep23462 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Gu, Chunsun
Song, Aiping
Zhang, Xiaoxue
Wang, Haibin
Li, Ting
Chen, Yu
Jiang, Jiafu
Chen, Fadi
Chen, Sumei
Cloning of chrysanthemum high-affinity nitrate transporter family (CmNRT2) and characterization of CmNRT2.1
title Cloning of chrysanthemum high-affinity nitrate transporter family (CmNRT2) and characterization of CmNRT2.1
title_full Cloning of chrysanthemum high-affinity nitrate transporter family (CmNRT2) and characterization of CmNRT2.1
title_fullStr Cloning of chrysanthemum high-affinity nitrate transporter family (CmNRT2) and characterization of CmNRT2.1
title_full_unstemmed Cloning of chrysanthemum high-affinity nitrate transporter family (CmNRT2) and characterization of CmNRT2.1
title_short Cloning of chrysanthemum high-affinity nitrate transporter family (CmNRT2) and characterization of CmNRT2.1
title_sort cloning of chrysanthemum high-affinity nitrate transporter family (cmnrt2) and characterization of cmnrt2.1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804277/
https://www.ncbi.nlm.nih.gov/pubmed/27004464
http://dx.doi.org/10.1038/srep23462
work_keys_str_mv AT guchunsun cloningofchrysanthemumhighaffinitynitratetransporterfamilycmnrt2andcharacterizationofcmnrt21
AT songaiping cloningofchrysanthemumhighaffinitynitratetransporterfamilycmnrt2andcharacterizationofcmnrt21
AT zhangxiaoxue cloningofchrysanthemumhighaffinitynitratetransporterfamilycmnrt2andcharacterizationofcmnrt21
AT wanghaibin cloningofchrysanthemumhighaffinitynitratetransporterfamilycmnrt2andcharacterizationofcmnrt21
AT liting cloningofchrysanthemumhighaffinitynitratetransporterfamilycmnrt2andcharacterizationofcmnrt21
AT chenyu cloningofchrysanthemumhighaffinitynitratetransporterfamilycmnrt2andcharacterizationofcmnrt21
AT jiangjiafu cloningofchrysanthemumhighaffinitynitratetransporterfamilycmnrt2andcharacterizationofcmnrt21
AT chenfadi cloningofchrysanthemumhighaffinitynitratetransporterfamilycmnrt2andcharacterizationofcmnrt21
AT chensumei cloningofchrysanthemumhighaffinitynitratetransporterfamilycmnrt2andcharacterizationofcmnrt21

Ejemplares similares