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Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury
Accumulating evidence indicates that peripheral nerve injury (PNI) activates spinal microglia that are necessary for neuropathic pain. Recent studies using bone marrow (BM) chimeric mice have reported that after PNI, circulating BM-derived cells infiltrate into the spinal cord and differentiate into...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804310/ https://www.ncbi.nlm.nih.gov/pubmed/27005516 http://dx.doi.org/10.1038/srep23701 |
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author | Tashima, Ryoichi Mikuriya, Satsuki Tomiyama, Daisuke Shiratori-Hayashi, Miho Yamashita, Tomohiro Kohro, Yuta Tozaki-Saitoh, Hidetoshi Inoue, Kazuhide Tsuda, Makoto |
author_facet | Tashima, Ryoichi Mikuriya, Satsuki Tomiyama, Daisuke Shiratori-Hayashi, Miho Yamashita, Tomohiro Kohro, Yuta Tozaki-Saitoh, Hidetoshi Inoue, Kazuhide Tsuda, Makoto |
author_sort | Tashima, Ryoichi |
collection | PubMed |
description | Accumulating evidence indicates that peripheral nerve injury (PNI) activates spinal microglia that are necessary for neuropathic pain. Recent studies using bone marrow (BM) chimeric mice have reported that after PNI, circulating BM-derived cells infiltrate into the spinal cord and differentiate into microglia-like cells. This raises the possibility that the population of spinal microglia after PNI may be heterogeneous. However, the infiltration of BM cells in the spinal cord remains controversial because of experimental adverse effects of strong irradiation used for generating BM chimeric mice. In this study, we evaluated the PNI-induced spinal infiltration of BM-derived cells not only by irradiation-induced myeloablation with various conditioning regimens, but also by parabiosis and mice with genetically labelled microglia, models without irradiation and BM transplantation. Results obtained from these independent approaches provide compelling evidence indicating little contribution of circulating BM-derived cells to the population of spinal microglia after PNI. |
format | Online Article Text |
id | pubmed-4804310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48043102016-03-24 Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury Tashima, Ryoichi Mikuriya, Satsuki Tomiyama, Daisuke Shiratori-Hayashi, Miho Yamashita, Tomohiro Kohro, Yuta Tozaki-Saitoh, Hidetoshi Inoue, Kazuhide Tsuda, Makoto Sci Rep Article Accumulating evidence indicates that peripheral nerve injury (PNI) activates spinal microglia that are necessary for neuropathic pain. Recent studies using bone marrow (BM) chimeric mice have reported that after PNI, circulating BM-derived cells infiltrate into the spinal cord and differentiate into microglia-like cells. This raises the possibility that the population of spinal microglia after PNI may be heterogeneous. However, the infiltration of BM cells in the spinal cord remains controversial because of experimental adverse effects of strong irradiation used for generating BM chimeric mice. In this study, we evaluated the PNI-induced spinal infiltration of BM-derived cells not only by irradiation-induced myeloablation with various conditioning regimens, but also by parabiosis and mice with genetically labelled microglia, models without irradiation and BM transplantation. Results obtained from these independent approaches provide compelling evidence indicating little contribution of circulating BM-derived cells to the population of spinal microglia after PNI. Nature Publishing Group 2016-03-23 /pmc/articles/PMC4804310/ /pubmed/27005516 http://dx.doi.org/10.1038/srep23701 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Tashima, Ryoichi Mikuriya, Satsuki Tomiyama, Daisuke Shiratori-Hayashi, Miho Yamashita, Tomohiro Kohro, Yuta Tozaki-Saitoh, Hidetoshi Inoue, Kazuhide Tsuda, Makoto Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury |
title | Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury |
title_full | Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury |
title_fullStr | Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury |
title_full_unstemmed | Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury |
title_short | Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury |
title_sort | bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804310/ https://www.ncbi.nlm.nih.gov/pubmed/27005516 http://dx.doi.org/10.1038/srep23701 |
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