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The chemosensory receptors of codling moth Cydia pomonella–expression in larvae and adults

Olfaction and gustation play critical roles in the life history of insects, mediating vital behaviors such as food, mate and host seeking. Chemosensory receptor proteins, including odorant receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs) function to interface the insect with...

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Autores principales: Walker, William B., Gonzalez, Francisco, Garczynski, Stephen F., Witzgall, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804390/
https://www.ncbi.nlm.nih.gov/pubmed/27006164
http://dx.doi.org/10.1038/srep23518
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author Walker, William B.
Gonzalez, Francisco
Garczynski, Stephen F.
Witzgall, Peter
author_facet Walker, William B.
Gonzalez, Francisco
Garczynski, Stephen F.
Witzgall, Peter
author_sort Walker, William B.
collection PubMed
description Olfaction and gustation play critical roles in the life history of insects, mediating vital behaviors such as food, mate and host seeking. Chemosensory receptor proteins, including odorant receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs) function to interface the insect with its chemical environment. Codling moth, Cydia pomonella, is a worldwide pest of apple, pear and walnut, and behavior-modifying semiochemicals are used for environmentally safe control. We produced an Illumina-based transcriptome from antennae of males and females as well as neonate head tissue, affording a qualitative and quantitative analysis of the codling moth chemosensory receptor repertoire. We identified 58 ORs, 20 GRs and 21 IRs, and provide a revised nomenclature that is consistent with homologous sequences in related species. Importantly, we have identified several OR transcripts displaying sex-biased expression in adults, as well as larval-enriched transcripts. Our analyses have expanded annotations of the chemosensory receptor gene families, and provide first-time transcript abundance estimates for codling moth. The results presented here provide a strong foundation for future work on codling moth behavioral physiology and ecology at the molecular level, and may lead to the development of more precise biorational control strategies.
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spelling pubmed-48043902016-03-24 The chemosensory receptors of codling moth Cydia pomonella–expression in larvae and adults Walker, William B. Gonzalez, Francisco Garczynski, Stephen F. Witzgall, Peter Sci Rep Article Olfaction and gustation play critical roles in the life history of insects, mediating vital behaviors such as food, mate and host seeking. Chemosensory receptor proteins, including odorant receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs) function to interface the insect with its chemical environment. Codling moth, Cydia pomonella, is a worldwide pest of apple, pear and walnut, and behavior-modifying semiochemicals are used for environmentally safe control. We produced an Illumina-based transcriptome from antennae of males and females as well as neonate head tissue, affording a qualitative and quantitative analysis of the codling moth chemosensory receptor repertoire. We identified 58 ORs, 20 GRs and 21 IRs, and provide a revised nomenclature that is consistent with homologous sequences in related species. Importantly, we have identified several OR transcripts displaying sex-biased expression in adults, as well as larval-enriched transcripts. Our analyses have expanded annotations of the chemosensory receptor gene families, and provide first-time transcript abundance estimates for codling moth. The results presented here provide a strong foundation for future work on codling moth behavioral physiology and ecology at the molecular level, and may lead to the development of more precise biorational control strategies. Nature Publishing Group 2016-03-23 /pmc/articles/PMC4804390/ /pubmed/27006164 http://dx.doi.org/10.1038/srep23518 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Walker, William B.
Gonzalez, Francisco
Garczynski, Stephen F.
Witzgall, Peter
The chemosensory receptors of codling moth Cydia pomonella–expression in larvae and adults
title The chemosensory receptors of codling moth Cydia pomonella–expression in larvae and adults
title_full The chemosensory receptors of codling moth Cydia pomonella–expression in larvae and adults
title_fullStr The chemosensory receptors of codling moth Cydia pomonella–expression in larvae and adults
title_full_unstemmed The chemosensory receptors of codling moth Cydia pomonella–expression in larvae and adults
title_short The chemosensory receptors of codling moth Cydia pomonella–expression in larvae and adults
title_sort chemosensory receptors of codling moth cydia pomonella–expression in larvae and adults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804390/
https://www.ncbi.nlm.nih.gov/pubmed/27006164
http://dx.doi.org/10.1038/srep23518
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