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Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats

The progression of diabetic nephropathy (DN) is accelerated by smoking. The current study investigated the ability of curcumin to protect the kidneys against damage from oxidative stress induced by diabetes mellitus (DM) and nicotine (NC). A total of 24 male Wistar rats were divided into four groups...

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Autores principales: IBRAHIM, ZEIN SHABAN, ALKAFAFY, MOHAMED ELSAYED, AHMED, MOHAMED MOHAMED, SOLIMAN, MOHAMED MOHAMED
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805097/
https://www.ncbi.nlm.nih.gov/pubmed/26936435
http://dx.doi.org/10.3892/mmr.2016.4922
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author IBRAHIM, ZEIN SHABAN
ALKAFAFY, MOHAMED ELSAYED
AHMED, MOHAMED MOHAMED
SOLIMAN, MOHAMED MOHAMED
author_facet IBRAHIM, ZEIN SHABAN
ALKAFAFY, MOHAMED ELSAYED
AHMED, MOHAMED MOHAMED
SOLIMAN, MOHAMED MOHAMED
author_sort IBRAHIM, ZEIN SHABAN
collection PubMed
description The progression of diabetic nephropathy (DN) is accelerated by smoking. The current study investigated the ability of curcumin to protect the kidneys against damage from oxidative stress induced by diabetes mellitus (DM) and nicotine (NC). A total of 24 male Wistar rats were divided into four groups of six rats each. DM was induced by a single intraperitoneal injection of streptozotocin 60 mg/kg body weight. DM rats were treated with or without NC in the absence or presence of curcumin for 8 weeks. As compared with the controls, DM rats exhibited reduced serum levels of high density lipoprotein, superoxide dismutase and glutathione peroxidase, and decreased renal mRNA expression levels of synaptopodin, connexin 43 and erythropoietin (EPO), which were further suppressed by NC and restored to normal levels by curcumin treatment. Additionally, DM rats exhibited increases in their lipid profiles (cholesterol, triacylglycerol and phospholipids), oxidative markers (malondialdehyde, γ-glutamyltranspeptidase and nitric oxide), kidney function markers (urea and creatinine) and the mRNA expression levels of vimentin, desmin, SREBP-1, iNOS and TGF-β1. These effects were further enhanced by NC, but counteracted by curcumin treatment. Kidneys from DM rats displayed glomerular hypertrophy, sclerosis and tubulo-interstitial changes represented by tubular lipid deposition, interstitial mononuclear cell infiltration and fibroplasia. Pancreatic islets exhibited cellular vacuolation, morphological irregularity and damaged or reduced in size β-cells. These renal and pancreatic changes became more severe following NC treatment and were ameliorated by curcumin. Therefore, NC-induced DN progression may predominantly operate by increasing oxidative stress, reducing the levels of antioxidants, suppressing EPO levels, and causing perturbations to gap junction and podocyte structure. Curcumin may ameliorate the damaging effects of DM and NC on the kidney through normalization of the mRNA expression levels of several genes important in the progression of DN.
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spelling pubmed-48050972016-04-04 Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats IBRAHIM, ZEIN SHABAN ALKAFAFY, MOHAMED ELSAYED AHMED, MOHAMED MOHAMED SOLIMAN, MOHAMED MOHAMED Mol Med Rep Articles The progression of diabetic nephropathy (DN) is accelerated by smoking. The current study investigated the ability of curcumin to protect the kidneys against damage from oxidative stress induced by diabetes mellitus (DM) and nicotine (NC). A total of 24 male Wistar rats were divided into four groups of six rats each. DM was induced by a single intraperitoneal injection of streptozotocin 60 mg/kg body weight. DM rats were treated with or without NC in the absence or presence of curcumin for 8 weeks. As compared with the controls, DM rats exhibited reduced serum levels of high density lipoprotein, superoxide dismutase and glutathione peroxidase, and decreased renal mRNA expression levels of synaptopodin, connexin 43 and erythropoietin (EPO), which were further suppressed by NC and restored to normal levels by curcumin treatment. Additionally, DM rats exhibited increases in their lipid profiles (cholesterol, triacylglycerol and phospholipids), oxidative markers (malondialdehyde, γ-glutamyltranspeptidase and nitric oxide), kidney function markers (urea and creatinine) and the mRNA expression levels of vimentin, desmin, SREBP-1, iNOS and TGF-β1. These effects were further enhanced by NC, but counteracted by curcumin treatment. Kidneys from DM rats displayed glomerular hypertrophy, sclerosis and tubulo-interstitial changes represented by tubular lipid deposition, interstitial mononuclear cell infiltration and fibroplasia. Pancreatic islets exhibited cellular vacuolation, morphological irregularity and damaged or reduced in size β-cells. These renal and pancreatic changes became more severe following NC treatment and were ameliorated by curcumin. Therefore, NC-induced DN progression may predominantly operate by increasing oxidative stress, reducing the levels of antioxidants, suppressing EPO levels, and causing perturbations to gap junction and podocyte structure. Curcumin may ameliorate the damaging effects of DM and NC on the kidney through normalization of the mRNA expression levels of several genes important in the progression of DN. D.A. Spandidos 2016-04 2016-02-22 /pmc/articles/PMC4805097/ /pubmed/26936435 http://dx.doi.org/10.3892/mmr.2016.4922 Text en Copyright: © Ibrahim et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
IBRAHIM, ZEIN SHABAN
ALKAFAFY, MOHAMED ELSAYED
AHMED, MOHAMED MOHAMED
SOLIMAN, MOHAMED MOHAMED
Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats
title Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats
title_full Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats
title_fullStr Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats
title_full_unstemmed Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats
title_short Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats
title_sort renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805097/
https://www.ncbi.nlm.nih.gov/pubmed/26936435
http://dx.doi.org/10.3892/mmr.2016.4922
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