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Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats
The progression of diabetic nephropathy (DN) is accelerated by smoking. The current study investigated the ability of curcumin to protect the kidneys against damage from oxidative stress induced by diabetes mellitus (DM) and nicotine (NC). A total of 24 male Wistar rats were divided into four groups...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805097/ https://www.ncbi.nlm.nih.gov/pubmed/26936435 http://dx.doi.org/10.3892/mmr.2016.4922 |
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author | IBRAHIM, ZEIN SHABAN ALKAFAFY, MOHAMED ELSAYED AHMED, MOHAMED MOHAMED SOLIMAN, MOHAMED MOHAMED |
author_facet | IBRAHIM, ZEIN SHABAN ALKAFAFY, MOHAMED ELSAYED AHMED, MOHAMED MOHAMED SOLIMAN, MOHAMED MOHAMED |
author_sort | IBRAHIM, ZEIN SHABAN |
collection | PubMed |
description | The progression of diabetic nephropathy (DN) is accelerated by smoking. The current study investigated the ability of curcumin to protect the kidneys against damage from oxidative stress induced by diabetes mellitus (DM) and nicotine (NC). A total of 24 male Wistar rats were divided into four groups of six rats each. DM was induced by a single intraperitoneal injection of streptozotocin 60 mg/kg body weight. DM rats were treated with or without NC in the absence or presence of curcumin for 8 weeks. As compared with the controls, DM rats exhibited reduced serum levels of high density lipoprotein, superoxide dismutase and glutathione peroxidase, and decreased renal mRNA expression levels of synaptopodin, connexin 43 and erythropoietin (EPO), which were further suppressed by NC and restored to normal levels by curcumin treatment. Additionally, DM rats exhibited increases in their lipid profiles (cholesterol, triacylglycerol and phospholipids), oxidative markers (malondialdehyde, γ-glutamyltranspeptidase and nitric oxide), kidney function markers (urea and creatinine) and the mRNA expression levels of vimentin, desmin, SREBP-1, iNOS and TGF-β1. These effects were further enhanced by NC, but counteracted by curcumin treatment. Kidneys from DM rats displayed glomerular hypertrophy, sclerosis and tubulo-interstitial changes represented by tubular lipid deposition, interstitial mononuclear cell infiltration and fibroplasia. Pancreatic islets exhibited cellular vacuolation, morphological irregularity and damaged or reduced in size β-cells. These renal and pancreatic changes became more severe following NC treatment and were ameliorated by curcumin. Therefore, NC-induced DN progression may predominantly operate by increasing oxidative stress, reducing the levels of antioxidants, suppressing EPO levels, and causing perturbations to gap junction and podocyte structure. Curcumin may ameliorate the damaging effects of DM and NC on the kidney through normalization of the mRNA expression levels of several genes important in the progression of DN. |
format | Online Article Text |
id | pubmed-4805097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-48050972016-04-04 Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats IBRAHIM, ZEIN SHABAN ALKAFAFY, MOHAMED ELSAYED AHMED, MOHAMED MOHAMED SOLIMAN, MOHAMED MOHAMED Mol Med Rep Articles The progression of diabetic nephropathy (DN) is accelerated by smoking. The current study investigated the ability of curcumin to protect the kidneys against damage from oxidative stress induced by diabetes mellitus (DM) and nicotine (NC). A total of 24 male Wistar rats were divided into four groups of six rats each. DM was induced by a single intraperitoneal injection of streptozotocin 60 mg/kg body weight. DM rats were treated with or without NC in the absence or presence of curcumin for 8 weeks. As compared with the controls, DM rats exhibited reduced serum levels of high density lipoprotein, superoxide dismutase and glutathione peroxidase, and decreased renal mRNA expression levels of synaptopodin, connexin 43 and erythropoietin (EPO), which were further suppressed by NC and restored to normal levels by curcumin treatment. Additionally, DM rats exhibited increases in their lipid profiles (cholesterol, triacylglycerol and phospholipids), oxidative markers (malondialdehyde, γ-glutamyltranspeptidase and nitric oxide), kidney function markers (urea and creatinine) and the mRNA expression levels of vimentin, desmin, SREBP-1, iNOS and TGF-β1. These effects were further enhanced by NC, but counteracted by curcumin treatment. Kidneys from DM rats displayed glomerular hypertrophy, sclerosis and tubulo-interstitial changes represented by tubular lipid deposition, interstitial mononuclear cell infiltration and fibroplasia. Pancreatic islets exhibited cellular vacuolation, morphological irregularity and damaged or reduced in size β-cells. These renal and pancreatic changes became more severe following NC treatment and were ameliorated by curcumin. Therefore, NC-induced DN progression may predominantly operate by increasing oxidative stress, reducing the levels of antioxidants, suppressing EPO levels, and causing perturbations to gap junction and podocyte structure. Curcumin may ameliorate the damaging effects of DM and NC on the kidney through normalization of the mRNA expression levels of several genes important in the progression of DN. D.A. Spandidos 2016-04 2016-02-22 /pmc/articles/PMC4805097/ /pubmed/26936435 http://dx.doi.org/10.3892/mmr.2016.4922 Text en Copyright: © Ibrahim et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles IBRAHIM, ZEIN SHABAN ALKAFAFY, MOHAMED ELSAYED AHMED, MOHAMED MOHAMED SOLIMAN, MOHAMED MOHAMED Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats |
title | Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats |
title_full | Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats |
title_fullStr | Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats |
title_full_unstemmed | Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats |
title_short | Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats |
title_sort | renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805097/ https://www.ncbi.nlm.nih.gov/pubmed/26936435 http://dx.doi.org/10.3892/mmr.2016.4922 |
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