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Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts

BACKGROUND/AIM: Wounds are the outcome of injuries to the skin that interrupt the soft tissue. Healing of a wound is a complex and long-drawn-out process of tissue repair and remodeling in response to injury. A large number of plants are used by folklore traditions for the treatment of cuts, wounds...

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Autores principales: Gothai, Sivapragasam, Arulselvan, Palanisamy, Tan, Woan Sean, Fakurazi, Sharida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGEYA 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805140/
https://www.ncbi.nlm.nih.gov/pubmed/27069722
http://dx.doi.org/10.5455/jice.20160201055629
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author Gothai, Sivapragasam
Arulselvan, Palanisamy
Tan, Woan Sean
Fakurazi, Sharida
author_facet Gothai, Sivapragasam
Arulselvan, Palanisamy
Tan, Woan Sean
Fakurazi, Sharida
author_sort Gothai, Sivapragasam
collection PubMed
description BACKGROUND/AIM: Wounds are the outcome of injuries to the skin that interrupt the soft tissue. Healing of a wound is a complex and long-drawn-out process of tissue repair and remodeling in response to injury. A large number of plants are used by folklore traditions for the treatment of cuts, wounds and burns. Moringa oleifera (MO) is an herb used as a traditional folk medicine for the treatment of various skin wounds and associated diseases. The underlying mechanisms of wound healing activity of ethyl acetate fraction of MO leaves extract are completely unknown. MATERIALS AND METHODS: In the current study, ethyl acetate fraction of MO leaves was investigated for its efficacy on cell viability, proliferation and migration (wound closure rate) in human normal dermal fibroblast cells. RESULTS: Results revealed that lower concentration (12.5 µg/ml, 25 µg/ml, and 50 µg/ml) of ethyl acetate fraction of MO leaves showed remarkable proliferative and migratory effect on normal human dermal fibroblasts. CONCLUSION: This study suggested that ethyl acetate fraction of MO leaves might be a potential therapeutic agent for skin wound healing by promoting fibroblast proliferation and migration through increasing the wound closure rate corroborating its traditional use.
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spelling pubmed-48051402016-04-11 Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts Gothai, Sivapragasam Arulselvan, Palanisamy Tan, Woan Sean Fakurazi, Sharida J Intercult Ethnopharmacol Original Research BACKGROUND/AIM: Wounds are the outcome of injuries to the skin that interrupt the soft tissue. Healing of a wound is a complex and long-drawn-out process of tissue repair and remodeling in response to injury. A large number of plants are used by folklore traditions for the treatment of cuts, wounds and burns. Moringa oleifera (MO) is an herb used as a traditional folk medicine for the treatment of various skin wounds and associated diseases. The underlying mechanisms of wound healing activity of ethyl acetate fraction of MO leaves extract are completely unknown. MATERIALS AND METHODS: In the current study, ethyl acetate fraction of MO leaves was investigated for its efficacy on cell viability, proliferation and migration (wound closure rate) in human normal dermal fibroblast cells. RESULTS: Results revealed that lower concentration (12.5 µg/ml, 25 µg/ml, and 50 µg/ml) of ethyl acetate fraction of MO leaves showed remarkable proliferative and migratory effect on normal human dermal fibroblasts. CONCLUSION: This study suggested that ethyl acetate fraction of MO leaves might be a potential therapeutic agent for skin wound healing by promoting fibroblast proliferation and migration through increasing the wound closure rate corroborating its traditional use. SAGEYA 2016-02-08 /pmc/articles/PMC4805140/ /pubmed/27069722 http://dx.doi.org/10.5455/jice.20160201055629 Text en Copyright: © SAGEYA http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, noncommercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Original Research
Gothai, Sivapragasam
Arulselvan, Palanisamy
Tan, Woan Sean
Fakurazi, Sharida
Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts
title Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts
title_full Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts
title_fullStr Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts
title_full_unstemmed Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts
title_short Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts
title_sort wound healing properties of ethyl acetate fraction of moringa oleifera in normal human dermal fibroblasts
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805140/
https://www.ncbi.nlm.nih.gov/pubmed/27069722
http://dx.doi.org/10.5455/jice.20160201055629
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