Sleep-active neuron specification and sleep induction require FLP-11 neuropeptides to systemically induce sleep

Sleep is an essential behavioral state. It is induced by conserved sleep-active neurons that express GABA. However, little is known about how sleep neuron function is determined and how sleep neurons change physiology and behavior systemically. Here, we investigated sleep in Caenorhabditis elegans,...

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Autores principales: Turek, Michal, Besseling, Judith, Spies, Jan-Philipp, König, Sabine, Bringmann, Henrik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805538/
https://www.ncbi.nlm.nih.gov/pubmed/26949257
http://dx.doi.org/10.7554/eLife.12499
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author Turek, Michal
Besseling, Judith
Spies, Jan-Philipp
König, Sabine
Bringmann, Henrik
author_facet Turek, Michal
Besseling, Judith
Spies, Jan-Philipp
König, Sabine
Bringmann, Henrik
author_sort Turek, Michal
collection PubMed
description Sleep is an essential behavioral state. It is induced by conserved sleep-active neurons that express GABA. However, little is known about how sleep neuron function is determined and how sleep neurons change physiology and behavior systemically. Here, we investigated sleep in Caenorhabditis elegans, which is induced by the single sleep-active neuron RIS. We found that the transcription factor LIM-6, which specifies GABAergic function, in parallel determines sleep neuron function through the expression of APTF-1, which specifies the expression of FLP-11 neuropeptides. Surprisingly FLP-11, and not GABA, is the major component that determines the sleep-promoting function of RIS. FLP-11 is constantly expressed in RIS. At sleep onset RIS depolarizes and releases FLP-11 to induce a systemic sleep state. DOI: http://dx.doi.org/10.7554/eLife.12499.001
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spelling pubmed-48055382016-03-25 Sleep-active neuron specification and sleep induction require FLP-11 neuropeptides to systemically induce sleep Turek, Michal Besseling, Judith Spies, Jan-Philipp König, Sabine Bringmann, Henrik eLife Neuroscience Sleep is an essential behavioral state. It is induced by conserved sleep-active neurons that express GABA. However, little is known about how sleep neuron function is determined and how sleep neurons change physiology and behavior systemically. Here, we investigated sleep in Caenorhabditis elegans, which is induced by the single sleep-active neuron RIS. We found that the transcription factor LIM-6, which specifies GABAergic function, in parallel determines sleep neuron function through the expression of APTF-1, which specifies the expression of FLP-11 neuropeptides. Surprisingly FLP-11, and not GABA, is the major component that determines the sleep-promoting function of RIS. FLP-11 is constantly expressed in RIS. At sleep onset RIS depolarizes and releases FLP-11 to induce a systemic sleep state. DOI: http://dx.doi.org/10.7554/eLife.12499.001 eLife Sciences Publications, Ltd 2016-03-07 /pmc/articles/PMC4805538/ /pubmed/26949257 http://dx.doi.org/10.7554/eLife.12499 Text en © 2016, Turek et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Turek, Michal
Besseling, Judith
Spies, Jan-Philipp
König, Sabine
Bringmann, Henrik
Sleep-active neuron specification and sleep induction require FLP-11 neuropeptides to systemically induce sleep
title Sleep-active neuron specification and sleep induction require FLP-11 neuropeptides to systemically induce sleep
title_full Sleep-active neuron specification and sleep induction require FLP-11 neuropeptides to systemically induce sleep
title_fullStr Sleep-active neuron specification and sleep induction require FLP-11 neuropeptides to systemically induce sleep
title_full_unstemmed Sleep-active neuron specification and sleep induction require FLP-11 neuropeptides to systemically induce sleep
title_short Sleep-active neuron specification and sleep induction require FLP-11 neuropeptides to systemically induce sleep
title_sort sleep-active neuron specification and sleep induction require flp-11 neuropeptides to systemically induce sleep
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805538/
https://www.ncbi.nlm.nih.gov/pubmed/26949257
http://dx.doi.org/10.7554/eLife.12499
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