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Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex

Motor coordination is supported by an array of highly organized heterogeneous modules in the cerebellum. How incoming sensorimotor information is channeled and communicated between these anatomical modules is still poorly understood. In this study, we used transgenic mice expressing GFP in specific...

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Autores principales: Valera, Antoine M, Binda, Francesca, Pawlowski, Sophie A, Dupont, Jean-Luc, Casella, Jean-François, Rothstein, Jeffrey D, Poulain, Bernard, Isope, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805550/
https://www.ncbi.nlm.nih.gov/pubmed/26982219
http://dx.doi.org/10.7554/eLife.09862
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author Valera, Antoine M
Binda, Francesca
Pawlowski, Sophie A
Dupont, Jean-Luc
Casella, Jean-François
Rothstein, Jeffrey D
Poulain, Bernard
Isope, Philippe
author_facet Valera, Antoine M
Binda, Francesca
Pawlowski, Sophie A
Dupont, Jean-Luc
Casella, Jean-François
Rothstein, Jeffrey D
Poulain, Bernard
Isope, Philippe
author_sort Valera, Antoine M
collection PubMed
description Motor coordination is supported by an array of highly organized heterogeneous modules in the cerebellum. How incoming sensorimotor information is channeled and communicated between these anatomical modules is still poorly understood. In this study, we used transgenic mice expressing GFP in specific subsets of Purkinje cells that allowed us to target a given set of cerebellar modules. Combining in vitro recordings and photostimulation, we identified stereotyped patterns of functional synaptic organization between the granule cell layer and its main targets, the Purkinje cells, Golgi cells and molecular layer interneurons. Each type of connection displayed position-specific patterns of granule cell synaptic inputs that do not strictly match with anatomical boundaries but connect distant cortical modules. Although these patterns can be adjusted by activity-dependent processes, they were found to be consistent and predictable between animals. Our results highlight the operational rules underlying communication between modules in the cerebellar cortex. DOI: http://dx.doi.org/10.7554/eLife.09862.001
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spelling pubmed-48055502016-03-25 Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex Valera, Antoine M Binda, Francesca Pawlowski, Sophie A Dupont, Jean-Luc Casella, Jean-François Rothstein, Jeffrey D Poulain, Bernard Isope, Philippe eLife Neuroscience Motor coordination is supported by an array of highly organized heterogeneous modules in the cerebellum. How incoming sensorimotor information is channeled and communicated between these anatomical modules is still poorly understood. In this study, we used transgenic mice expressing GFP in specific subsets of Purkinje cells that allowed us to target a given set of cerebellar modules. Combining in vitro recordings and photostimulation, we identified stereotyped patterns of functional synaptic organization between the granule cell layer and its main targets, the Purkinje cells, Golgi cells and molecular layer interneurons. Each type of connection displayed position-specific patterns of granule cell synaptic inputs that do not strictly match with anatomical boundaries but connect distant cortical modules. Although these patterns can be adjusted by activity-dependent processes, they were found to be consistent and predictable between animals. Our results highlight the operational rules underlying communication between modules in the cerebellar cortex. DOI: http://dx.doi.org/10.7554/eLife.09862.001 eLife Sciences Publications, Ltd 2016-03-16 /pmc/articles/PMC4805550/ /pubmed/26982219 http://dx.doi.org/10.7554/eLife.09862 Text en © 2016, Valera et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Valera, Antoine M
Binda, Francesca
Pawlowski, Sophie A
Dupont, Jean-Luc
Casella, Jean-François
Rothstein, Jeffrey D
Poulain, Bernard
Isope, Philippe
Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex
title Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex
title_full Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex
title_fullStr Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex
title_full_unstemmed Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex
title_short Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex
title_sort stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805550/
https://www.ncbi.nlm.nih.gov/pubmed/26982219
http://dx.doi.org/10.7554/eLife.09862
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