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Biocompatibility of magnesium implants in primary human reaming debris-derived cells stem cells in vitro
BACKGROUND: Use of magnesium for resorbable metal implants is a new concept in orthopaedic and dental medicine. The majority of studies on magnesium’s biocompatibility in vitro have assessed the short-term effect of magnesium extract on cells. The aim of this study was to evaluate the influence of d...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805638/ https://www.ncbi.nlm.nih.gov/pubmed/26153416 http://dx.doi.org/10.1007/s10195-015-0364-9 |
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author | Charyeva, Olga Dakischew, Olga Sommer, Ursula Heiss, Christian Schnettler, Reinhard Lips, Katrin Susanne |
author_facet | Charyeva, Olga Dakischew, Olga Sommer, Ursula Heiss, Christian Schnettler, Reinhard Lips, Katrin Susanne |
author_sort | Charyeva, Olga |
collection | PubMed |
description | BACKGROUND: Use of magnesium for resorbable metal implants is a new concept in orthopaedic and dental medicine. The majority of studies on magnesium’s biocompatibility in vitro have assessed the short-term effect of magnesium extract on cells. The aim of this study was to evaluate the influence of direct exposure to magnesium alloys on the bioactivity of primary human reaming debris-derived (HRD) cells. MATERIALS AND METHODS: Pure Mg, Mg2Ag, WE43 and Mg10Gd were tested for biocompatibility. The study consisted of assessment of cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, evaluation of alkaline phosphatase (ALP) content, and study of cell morphology under light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM), along with determination of calcification and pH changes induced by magnesium. RESULTS: The number of viable cells in the presence of Mg2Ag was high over the entire observation period. Inhibition of ALP content in osteogenic differentiating HRD was caused by pure Mg at day 14 and 28. All other magnesium alloys did not affect the ALP content. Exposure of HRD to magnesium increased the amount of lysosomes and endocytotic vesicles. Cellular attachment was generally the best for those crystals that formed on the surface of all materials. A decrease was observed in Ca(2+) in the medium from day 1 to day 14. CONCLUSIONS: In terms of cell morphology, cell viability and differentiation, cell density and the effect on the surrounding pH, Mg2Ag showed the most promising results. All magnesium materials induced calcification, which is beneficial for orthopaedic and dental applications. |
format | Online Article Text |
id | pubmed-4805638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-48056382016-04-09 Biocompatibility of magnesium implants in primary human reaming debris-derived cells stem cells in vitro Charyeva, Olga Dakischew, Olga Sommer, Ursula Heiss, Christian Schnettler, Reinhard Lips, Katrin Susanne J Orthop Traumatol Original Article BACKGROUND: Use of magnesium for resorbable metal implants is a new concept in orthopaedic and dental medicine. The majority of studies on magnesium’s biocompatibility in vitro have assessed the short-term effect of magnesium extract on cells. The aim of this study was to evaluate the influence of direct exposure to magnesium alloys on the bioactivity of primary human reaming debris-derived (HRD) cells. MATERIALS AND METHODS: Pure Mg, Mg2Ag, WE43 and Mg10Gd were tested for biocompatibility. The study consisted of assessment of cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, evaluation of alkaline phosphatase (ALP) content, and study of cell morphology under light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM), along with determination of calcification and pH changes induced by magnesium. RESULTS: The number of viable cells in the presence of Mg2Ag was high over the entire observation period. Inhibition of ALP content in osteogenic differentiating HRD was caused by pure Mg at day 14 and 28. All other magnesium alloys did not affect the ALP content. Exposure of HRD to magnesium increased the amount of lysosomes and endocytotic vesicles. Cellular attachment was generally the best for those crystals that formed on the surface of all materials. A decrease was observed in Ca(2+) in the medium from day 1 to day 14. CONCLUSIONS: In terms of cell morphology, cell viability and differentiation, cell density and the effect on the surrounding pH, Mg2Ag showed the most promising results. All magnesium materials induced calcification, which is beneficial for orthopaedic and dental applications. Springer International Publishing 2015-07-08 2016-03 /pmc/articles/PMC4805638/ /pubmed/26153416 http://dx.doi.org/10.1007/s10195-015-0364-9 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Charyeva, Olga Dakischew, Olga Sommer, Ursula Heiss, Christian Schnettler, Reinhard Lips, Katrin Susanne Biocompatibility of magnesium implants in primary human reaming debris-derived cells stem cells in vitro |
title | Biocompatibility of magnesium implants in primary human reaming debris-derived cells stem cells in vitro |
title_full | Biocompatibility of magnesium implants in primary human reaming debris-derived cells stem cells in vitro |
title_fullStr | Biocompatibility of magnesium implants in primary human reaming debris-derived cells stem cells in vitro |
title_full_unstemmed | Biocompatibility of magnesium implants in primary human reaming debris-derived cells stem cells in vitro |
title_short | Biocompatibility of magnesium implants in primary human reaming debris-derived cells stem cells in vitro |
title_sort | biocompatibility of magnesium implants in primary human reaming debris-derived cells stem cells in vitro |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805638/ https://www.ncbi.nlm.nih.gov/pubmed/26153416 http://dx.doi.org/10.1007/s10195-015-0364-9 |
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