Morphotype-specific effector functions of Cryptococcus neoformans PUM1

The basidiomycete fungal pathogen Cryptococcus neoformans requires the PUF protein, Pum1, for hyphal morphogenesis during sexual development. In this study we found that Pum1 was auto-repressive under growth as yeast, but that auto-repression was relieved during filamentous growth through utilizatio...

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Autores principales: Kaur, Jan Naseer, Panepinto, John C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4806291/
https://www.ncbi.nlm.nih.gov/pubmed/27008977
http://dx.doi.org/10.1038/srep23638
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author Kaur, Jan Naseer
Panepinto, John C.
author_facet Kaur, Jan Naseer
Panepinto, John C.
author_sort Kaur, Jan Naseer
collection PubMed
description The basidiomycete fungal pathogen Cryptococcus neoformans requires the PUF protein, Pum1, for hyphal morphogenesis during sexual development. In this study we found that Pum1 was auto-repressive under growth as yeast, but that auto-repression was relieved during filamentous growth through utilization of an alternative transcription start site driven by the master filamentation regulator Znf2. In addition, Pum1 was required to stabilize the ZNF2 mRNA through an indirect mechanism suggesting that Znf2 and Pum1 each positively regulate the expression of the other to achieve the filamentous morphotype required for sexual development in Cryptococcus.
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spelling pubmed-48062912016-03-24 Morphotype-specific effector functions of Cryptococcus neoformans PUM1 Kaur, Jan Naseer Panepinto, John C. Sci Rep Article The basidiomycete fungal pathogen Cryptococcus neoformans requires the PUF protein, Pum1, for hyphal morphogenesis during sexual development. In this study we found that Pum1 was auto-repressive under growth as yeast, but that auto-repression was relieved during filamentous growth through utilization of an alternative transcription start site driven by the master filamentation regulator Znf2. In addition, Pum1 was required to stabilize the ZNF2 mRNA through an indirect mechanism suggesting that Znf2 and Pum1 each positively regulate the expression of the other to achieve the filamentous morphotype required for sexual development in Cryptococcus. Nature Publishing Group 2016-03-24 /pmc/articles/PMC4806291/ /pubmed/27008977 http://dx.doi.org/10.1038/srep23638 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kaur, Jan Naseer
Panepinto, John C.
Morphotype-specific effector functions of Cryptococcus neoformans PUM1
title Morphotype-specific effector functions of Cryptococcus neoformans PUM1
title_full Morphotype-specific effector functions of Cryptococcus neoformans PUM1
title_fullStr Morphotype-specific effector functions of Cryptococcus neoformans PUM1
title_full_unstemmed Morphotype-specific effector functions of Cryptococcus neoformans PUM1
title_short Morphotype-specific effector functions of Cryptococcus neoformans PUM1
title_sort morphotype-specific effector functions of cryptococcus neoformans pum1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4806291/
https://www.ncbi.nlm.nih.gov/pubmed/27008977
http://dx.doi.org/10.1038/srep23638
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