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miR-isomiRExp: a web-server for the analysis of expression of miRNA at the miRNA/isomiR levels

MicroRNA (miRNA) locus has been found that can generate a series of varied isomiR sequences. Most studies always focus on determining miRNA level, however, the canonical miRNA sequence is only a specific member in the multiple isomiRs. Some studies have shown that isomiR sequences play versatile rol...

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Detalles Bibliográficos
Autores principales: Guo, Li, Yu, Jiafeng, Liang, Tingming, Zou, Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4806314/
https://www.ncbi.nlm.nih.gov/pubmed/27009551
http://dx.doi.org/10.1038/srep23700
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author Guo, Li
Yu, Jiafeng
Liang, Tingming
Zou, Quan
author_facet Guo, Li
Yu, Jiafeng
Liang, Tingming
Zou, Quan
author_sort Guo, Li
collection PubMed
description MicroRNA (miRNA) locus has been found that can generate a series of varied isomiR sequences. Most studies always focus on determining miRNA level, however, the canonical miRNA sequence is only a specific member in the multiple isomiRs. Some studies have shown that isomiR sequences play versatile roles in biological progress, and the analysis and research should be simultaneously performed at the miRNA/isomiR levels. Based on the biological characteristics of miRNA and isomiR, we developed miR-isomiRExp to analyze expression pattern of miRNA at the miRNA/isomiR levels, provide insights into tracking miRNA/isomiR maturation and processing mechanisms, and reveal functional characteristics of miRNA/isomiR. Simultaneously, we also performed expression analysis of specific human diseases using public small RNA sequencing datasets based on the analysis platform, which may help in surveying the potential deregulated miRNA/isomiR expression profiles, especially sequence and function-related isomiRs for further interaction analysis and study. The miR-isomiRExp platform provides miRNA/isomiR expression patterns and more information to study deregulated miRNA loci and detailed isomiR sequences. This comprehensive analysis will enrich experimental miRNA studies. miR-isomiRExp is available at http://server.malab.cn/miRisomiRExp/.
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spelling pubmed-48063142016-03-24 miR-isomiRExp: a web-server for the analysis of expression of miRNA at the miRNA/isomiR levels Guo, Li Yu, Jiafeng Liang, Tingming Zou, Quan Sci Rep Article MicroRNA (miRNA) locus has been found that can generate a series of varied isomiR sequences. Most studies always focus on determining miRNA level, however, the canonical miRNA sequence is only a specific member in the multiple isomiRs. Some studies have shown that isomiR sequences play versatile roles in biological progress, and the analysis and research should be simultaneously performed at the miRNA/isomiR levels. Based on the biological characteristics of miRNA and isomiR, we developed miR-isomiRExp to analyze expression pattern of miRNA at the miRNA/isomiR levels, provide insights into tracking miRNA/isomiR maturation and processing mechanisms, and reveal functional characteristics of miRNA/isomiR. Simultaneously, we also performed expression analysis of specific human diseases using public small RNA sequencing datasets based on the analysis platform, which may help in surveying the potential deregulated miRNA/isomiR expression profiles, especially sequence and function-related isomiRs for further interaction analysis and study. The miR-isomiRExp platform provides miRNA/isomiR expression patterns and more information to study deregulated miRNA loci and detailed isomiR sequences. This comprehensive analysis will enrich experimental miRNA studies. miR-isomiRExp is available at http://server.malab.cn/miRisomiRExp/. Nature Publishing Group 2016-03-24 /pmc/articles/PMC4806314/ /pubmed/27009551 http://dx.doi.org/10.1038/srep23700 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Guo, Li
Yu, Jiafeng
Liang, Tingming
Zou, Quan
miR-isomiRExp: a web-server for the analysis of expression of miRNA at the miRNA/isomiR levels
title miR-isomiRExp: a web-server for the analysis of expression of miRNA at the miRNA/isomiR levels
title_full miR-isomiRExp: a web-server for the analysis of expression of miRNA at the miRNA/isomiR levels
title_fullStr miR-isomiRExp: a web-server for the analysis of expression of miRNA at the miRNA/isomiR levels
title_full_unstemmed miR-isomiRExp: a web-server for the analysis of expression of miRNA at the miRNA/isomiR levels
title_short miR-isomiRExp: a web-server for the analysis of expression of miRNA at the miRNA/isomiR levels
title_sort mir-isomirexp: a web-server for the analysis of expression of mirna at the mirna/isomir levels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4806314/
https://www.ncbi.nlm.nih.gov/pubmed/27009551
http://dx.doi.org/10.1038/srep23700
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