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Microevolution of Monophasic Salmonella Typhimurium during Epidemic, United Kingdom, 2005–2010

Microevolution associated with emergence and expansion of new epidemic clones of bacterial pathogens holds the key to epidemiologic success. To determine microevolution associated with monophasic Salmonella Typhimurium during an epidemic, we performed comparative whole-genome sequencing and phylogen...

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Autores principales: Petrovska, Liljana, Mather, Alison E., AbuOun, Manal, Branchu, Priscilla, Harris, Simon R., Connor, Thomas, Hopkins, K.L., Underwood, A., Lettini, Antonia A., Page, Andrew, Bagnall, Mary, Wain, John, Parkhill, Julian, Dougan, Gordon, Davies, Robert, Kingsley, Robert A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4806966/
https://www.ncbi.nlm.nih.gov/pubmed/26982594
http://dx.doi.org/10.3201/eid2204.150531
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author Petrovska, Liljana
Mather, Alison E.
AbuOun, Manal
Branchu, Priscilla
Harris, Simon R.
Connor, Thomas
Hopkins, K.L.
Underwood, A.
Lettini, Antonia A.
Page, Andrew
Bagnall, Mary
Wain, John
Parkhill, Julian
Dougan, Gordon
Davies, Robert
Kingsley, Robert A.
author_facet Petrovska, Liljana
Mather, Alison E.
AbuOun, Manal
Branchu, Priscilla
Harris, Simon R.
Connor, Thomas
Hopkins, K.L.
Underwood, A.
Lettini, Antonia A.
Page, Andrew
Bagnall, Mary
Wain, John
Parkhill, Julian
Dougan, Gordon
Davies, Robert
Kingsley, Robert A.
author_sort Petrovska, Liljana
collection PubMed
description Microevolution associated with emergence and expansion of new epidemic clones of bacterial pathogens holds the key to epidemiologic success. To determine microevolution associated with monophasic Salmonella Typhimurium during an epidemic, we performed comparative whole-genome sequencing and phylogenomic analysis of isolates from the United Kingdom and Italy during 2005–2012. These isolates formed a single clade distinct from recent monophasic epidemic clones previously described from North America and Spain. The UK monophasic epidemic clones showed a novel genomic island encoding resistance to heavy metals and a composite transposon encoding antimicrobial drug resistance genes not present in other Salmonella Typhimurium isolates, which may have contributed to epidemiologic success. A remarkable amount of genotypic variation accumulated during clonal expansion that occurred during the epidemic, including multiple independent acquisitions of a novel prophage carrying the sopE gene and multiple deletion events affecting the phase II flagellin locus. This high level of microevolution may affect antigenicity, pathogenicity, and transmission.
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spelling pubmed-48069662016-04-01 Microevolution of Monophasic Salmonella Typhimurium during Epidemic, United Kingdom, 2005–2010 Petrovska, Liljana Mather, Alison E. AbuOun, Manal Branchu, Priscilla Harris, Simon R. Connor, Thomas Hopkins, K.L. Underwood, A. Lettini, Antonia A. Page, Andrew Bagnall, Mary Wain, John Parkhill, Julian Dougan, Gordon Davies, Robert Kingsley, Robert A. Emerg Infect Dis Research Microevolution associated with emergence and expansion of new epidemic clones of bacterial pathogens holds the key to epidemiologic success. To determine microevolution associated with monophasic Salmonella Typhimurium during an epidemic, we performed comparative whole-genome sequencing and phylogenomic analysis of isolates from the United Kingdom and Italy during 2005–2012. These isolates formed a single clade distinct from recent monophasic epidemic clones previously described from North America and Spain. The UK monophasic epidemic clones showed a novel genomic island encoding resistance to heavy metals and a composite transposon encoding antimicrobial drug resistance genes not present in other Salmonella Typhimurium isolates, which may have contributed to epidemiologic success. A remarkable amount of genotypic variation accumulated during clonal expansion that occurred during the epidemic, including multiple independent acquisitions of a novel prophage carrying the sopE gene and multiple deletion events affecting the phase II flagellin locus. This high level of microevolution may affect antigenicity, pathogenicity, and transmission. Centers for Disease Control and Prevention 2016-04 /pmc/articles/PMC4806966/ /pubmed/26982594 http://dx.doi.org/10.3201/eid2204.150531 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Petrovska, Liljana
Mather, Alison E.
AbuOun, Manal
Branchu, Priscilla
Harris, Simon R.
Connor, Thomas
Hopkins, K.L.
Underwood, A.
Lettini, Antonia A.
Page, Andrew
Bagnall, Mary
Wain, John
Parkhill, Julian
Dougan, Gordon
Davies, Robert
Kingsley, Robert A.
Microevolution of Monophasic Salmonella Typhimurium during Epidemic, United Kingdom, 2005–2010
title Microevolution of Monophasic Salmonella Typhimurium during Epidemic, United Kingdom, 2005–2010
title_full Microevolution of Monophasic Salmonella Typhimurium during Epidemic, United Kingdom, 2005–2010
title_fullStr Microevolution of Monophasic Salmonella Typhimurium during Epidemic, United Kingdom, 2005–2010
title_full_unstemmed Microevolution of Monophasic Salmonella Typhimurium during Epidemic, United Kingdom, 2005–2010
title_short Microevolution of Monophasic Salmonella Typhimurium during Epidemic, United Kingdom, 2005–2010
title_sort microevolution of monophasic salmonella typhimurium during epidemic, united kingdom, 2005–2010
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4806966/
https://www.ncbi.nlm.nih.gov/pubmed/26982594
http://dx.doi.org/10.3201/eid2204.150531
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