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Anti-Human VEGF Repebody Effectively Suppresses Choroidal Neovascularization and Vascular Leakage
Age-related macular degeneration (AMD) is the leading cause of vision loss and blindness among people over the age of 60. Vascular endothelial growth factor (VEGF) plays a major role in pathological angiogenesis in AMD. Herein, we present the development of an anti- human VEGF repebody, which is a s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4807815/ https://www.ncbi.nlm.nih.gov/pubmed/27015541 http://dx.doi.org/10.1371/journal.pone.0152522 |
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author | Hwang, Da-Eun Ryou, Jeong-Hyun Oh, Jong Rok Han, Jung Woo Park, Tae Kwann Kim, Hak-Sung |
author_facet | Hwang, Da-Eun Ryou, Jeong-Hyun Oh, Jong Rok Han, Jung Woo Park, Tae Kwann Kim, Hak-Sung |
author_sort | Hwang, Da-Eun |
collection | PubMed |
description | Age-related macular degeneration (AMD) is the leading cause of vision loss and blindness among people over the age of 60. Vascular endothelial growth factor (VEGF) plays a major role in pathological angiogenesis in AMD. Herein, we present the development of an anti- human VEGF repebody, which is a small-sized protein binder consisting of leucine-rich repeat (LRR) modules. The anti-VEGF repebody selected through a phage-display was shown to have a high affinity and specificity for human VEGF. We demonstrate that this repebody effectively inhibits in vitro angiogenic cellular processes, such as proliferation and migration, by blocking the VEGF-mediated signaling pathway. The repebody was also shown to have a strong suppression effect on choroidal neovascularization (CNV) and vascular leakage in vivo. Our results indicate that the anti-VEGF repebody has a therapeutic potential for treating neovascular AMD as well as other VEGF-involved diseases including diabetic retinopathy and metastatic cancers. |
format | Online Article Text |
id | pubmed-4807815 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48078152016-04-05 Anti-Human VEGF Repebody Effectively Suppresses Choroidal Neovascularization and Vascular Leakage Hwang, Da-Eun Ryou, Jeong-Hyun Oh, Jong Rok Han, Jung Woo Park, Tae Kwann Kim, Hak-Sung PLoS One Research Article Age-related macular degeneration (AMD) is the leading cause of vision loss and blindness among people over the age of 60. Vascular endothelial growth factor (VEGF) plays a major role in pathological angiogenesis in AMD. Herein, we present the development of an anti- human VEGF repebody, which is a small-sized protein binder consisting of leucine-rich repeat (LRR) modules. The anti-VEGF repebody selected through a phage-display was shown to have a high affinity and specificity for human VEGF. We demonstrate that this repebody effectively inhibits in vitro angiogenic cellular processes, such as proliferation and migration, by blocking the VEGF-mediated signaling pathway. The repebody was also shown to have a strong suppression effect on choroidal neovascularization (CNV) and vascular leakage in vivo. Our results indicate that the anti-VEGF repebody has a therapeutic potential for treating neovascular AMD as well as other VEGF-involved diseases including diabetic retinopathy and metastatic cancers. Public Library of Science 2016-03-25 /pmc/articles/PMC4807815/ /pubmed/27015541 http://dx.doi.org/10.1371/journal.pone.0152522 Text en © 2016 Hwang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hwang, Da-Eun Ryou, Jeong-Hyun Oh, Jong Rok Han, Jung Woo Park, Tae Kwann Kim, Hak-Sung Anti-Human VEGF Repebody Effectively Suppresses Choroidal Neovascularization and Vascular Leakage |
title | Anti-Human VEGF Repebody Effectively Suppresses Choroidal Neovascularization and Vascular Leakage |
title_full | Anti-Human VEGF Repebody Effectively Suppresses Choroidal Neovascularization and Vascular Leakage |
title_fullStr | Anti-Human VEGF Repebody Effectively Suppresses Choroidal Neovascularization and Vascular Leakage |
title_full_unstemmed | Anti-Human VEGF Repebody Effectively Suppresses Choroidal Neovascularization and Vascular Leakage |
title_short | Anti-Human VEGF Repebody Effectively Suppresses Choroidal Neovascularization and Vascular Leakage |
title_sort | anti-human vegf repebody effectively suppresses choroidal neovascularization and vascular leakage |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4807815/ https://www.ncbi.nlm.nih.gov/pubmed/27015541 http://dx.doi.org/10.1371/journal.pone.0152522 |
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