Cargando…

Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice

Reactive oxygen species (ROS) are important in the pathogenesis of pulmonary injury induced by cigarette smoke (CS) exposure, and physical exercise (Ex) is useful in combating impaired oxidative process. We verified the preventive effects of Ex on lung oxidative markers induced by smoking. In this s...

Descripción completa

Detalles Bibliográficos
Autores principales: Nesi, Renata Tiscoski, de Souza, Priscila Soares, dos Santos, Giulia Pedroso, Thirupathi, Anand, Menegali, Bruno T, Silveira, Paulo Cesar Lock, da Silva, Luciano Acordi, Valença, Samuel Santos, Pinho, Ricardo Aurino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809330/
https://www.ncbi.nlm.nih.gov/pubmed/27042047
http://dx.doi.org/10.2147/COPD.S93958
_version_ 1782423620595744768
author Nesi, Renata Tiscoski
de Souza, Priscila Soares
dos Santos, Giulia Pedroso
Thirupathi, Anand
Menegali, Bruno T
Silveira, Paulo Cesar Lock
da Silva, Luciano Acordi
Valença, Samuel Santos
Pinho, Ricardo Aurino
author_facet Nesi, Renata Tiscoski
de Souza, Priscila Soares
dos Santos, Giulia Pedroso
Thirupathi, Anand
Menegali, Bruno T
Silveira, Paulo Cesar Lock
da Silva, Luciano Acordi
Valença, Samuel Santos
Pinho, Ricardo Aurino
author_sort Nesi, Renata Tiscoski
collection PubMed
description Reactive oxygen species (ROS) are important in the pathogenesis of pulmonary injury induced by cigarette smoke (CS) exposure, and physical exercise (Ex) is useful in combating impaired oxidative process. We verified the preventive effects of Ex on lung oxidative markers induced by smoking. In this study, 36 mice (C57BL-6, 30–35 g) were split into four groups: control, CS, Ex, and CS plus Ex. Ex groups were given prior physical training in water (2×30 min/d, 5 days/wk, 8 weeks). After training, the CS groups were subjected to passive exposure to four cigarettes, 3 × per day, for 60 consecutive days. After 24 hours from the last exposure, CS animals were sacrificed, and lung samples were collected for further analysis. Left lung sample was prepared for histological analysis, and right lung was used for biochemical analysis (superoxide, hydroxyproline, lipid peroxidation [thiobarbituric acid reactive species], protein carbonylation [carbonyl groups formation], superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx] activities). Group comparisons were evaluated by analysis of variance (ANOVA). Results were expressed as mean ± standard deviation, with P<0.05 considered significantly different. Preventive Ex impeded histological changes and increased the enzymatic defense system (SOD and GPx) by reducing oxidative damage in lipids and proteins. This preventive effect of prior physical Ex alleviates damage caused by CS exposure.
format Online
Article
Text
id pubmed-4809330
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-48093302016-04-01 Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice Nesi, Renata Tiscoski de Souza, Priscila Soares dos Santos, Giulia Pedroso Thirupathi, Anand Menegali, Bruno T Silveira, Paulo Cesar Lock da Silva, Luciano Acordi Valença, Samuel Santos Pinho, Ricardo Aurino Int J Chron Obstruct Pulmon Dis Original Research Reactive oxygen species (ROS) are important in the pathogenesis of pulmonary injury induced by cigarette smoke (CS) exposure, and physical exercise (Ex) is useful in combating impaired oxidative process. We verified the preventive effects of Ex on lung oxidative markers induced by smoking. In this study, 36 mice (C57BL-6, 30–35 g) were split into four groups: control, CS, Ex, and CS plus Ex. Ex groups were given prior physical training in water (2×30 min/d, 5 days/wk, 8 weeks). After training, the CS groups were subjected to passive exposure to four cigarettes, 3 × per day, for 60 consecutive days. After 24 hours from the last exposure, CS animals were sacrificed, and lung samples were collected for further analysis. Left lung sample was prepared for histological analysis, and right lung was used for biochemical analysis (superoxide, hydroxyproline, lipid peroxidation [thiobarbituric acid reactive species], protein carbonylation [carbonyl groups formation], superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx] activities). Group comparisons were evaluated by analysis of variance (ANOVA). Results were expressed as mean ± standard deviation, with P<0.05 considered significantly different. Preventive Ex impeded histological changes and increased the enzymatic defense system (SOD and GPx) by reducing oxidative damage in lipids and proteins. This preventive effect of prior physical Ex alleviates damage caused by CS exposure. Dove Medical Press 2016-03-22 /pmc/articles/PMC4809330/ /pubmed/27042047 http://dx.doi.org/10.2147/COPD.S93958 Text en © 2016 Nesi et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Nesi, Renata Tiscoski
de Souza, Priscila Soares
dos Santos, Giulia Pedroso
Thirupathi, Anand
Menegali, Bruno T
Silveira, Paulo Cesar Lock
da Silva, Luciano Acordi
Valença, Samuel Santos
Pinho, Ricardo Aurino
Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice
title Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice
title_full Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice
title_fullStr Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice
title_full_unstemmed Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice
title_short Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice
title_sort physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809330/
https://www.ncbi.nlm.nih.gov/pubmed/27042047
http://dx.doi.org/10.2147/COPD.S93958
work_keys_str_mv AT nesirenatatiscoski physicalexerciseiseffectiveinpreventingcigarettesmokeinducedpulmonaryoxidativeresponseinmice
AT desouzapriscilasoares physicalexerciseiseffectiveinpreventingcigarettesmokeinducedpulmonaryoxidativeresponseinmice
AT dossantosgiuliapedroso physicalexerciseiseffectiveinpreventingcigarettesmokeinducedpulmonaryoxidativeresponseinmice
AT thirupathianand physicalexerciseiseffectiveinpreventingcigarettesmokeinducedpulmonaryoxidativeresponseinmice
AT menegalibrunot physicalexerciseiseffectiveinpreventingcigarettesmokeinducedpulmonaryoxidativeresponseinmice
AT silveirapaulocesarlock physicalexerciseiseffectiveinpreventingcigarettesmokeinducedpulmonaryoxidativeresponseinmice
AT dasilvalucianoacordi physicalexerciseiseffectiveinpreventingcigarettesmokeinducedpulmonaryoxidativeresponseinmice
AT valencasamuelsantos physicalexerciseiseffectiveinpreventingcigarettesmokeinducedpulmonaryoxidativeresponseinmice
AT pinhoricardoaurino physicalexerciseiseffectiveinpreventingcigarettesmokeinducedpulmonaryoxidativeresponseinmice