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Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice
Reactive oxygen species (ROS) are important in the pathogenesis of pulmonary injury induced by cigarette smoke (CS) exposure, and physical exercise (Ex) is useful in combating impaired oxidative process. We verified the preventive effects of Ex on lung oxidative markers induced by smoking. In this s...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809330/ https://www.ncbi.nlm.nih.gov/pubmed/27042047 http://dx.doi.org/10.2147/COPD.S93958 |
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author | Nesi, Renata Tiscoski de Souza, Priscila Soares dos Santos, Giulia Pedroso Thirupathi, Anand Menegali, Bruno T Silveira, Paulo Cesar Lock da Silva, Luciano Acordi Valença, Samuel Santos Pinho, Ricardo Aurino |
author_facet | Nesi, Renata Tiscoski de Souza, Priscila Soares dos Santos, Giulia Pedroso Thirupathi, Anand Menegali, Bruno T Silveira, Paulo Cesar Lock da Silva, Luciano Acordi Valença, Samuel Santos Pinho, Ricardo Aurino |
author_sort | Nesi, Renata Tiscoski |
collection | PubMed |
description | Reactive oxygen species (ROS) are important in the pathogenesis of pulmonary injury induced by cigarette smoke (CS) exposure, and physical exercise (Ex) is useful in combating impaired oxidative process. We verified the preventive effects of Ex on lung oxidative markers induced by smoking. In this study, 36 mice (C57BL-6, 30–35 g) were split into four groups: control, CS, Ex, and CS plus Ex. Ex groups were given prior physical training in water (2×30 min/d, 5 days/wk, 8 weeks). After training, the CS groups were subjected to passive exposure to four cigarettes, 3 × per day, for 60 consecutive days. After 24 hours from the last exposure, CS animals were sacrificed, and lung samples were collected for further analysis. Left lung sample was prepared for histological analysis, and right lung was used for biochemical analysis (superoxide, hydroxyproline, lipid peroxidation [thiobarbituric acid reactive species], protein carbonylation [carbonyl groups formation], superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx] activities). Group comparisons were evaluated by analysis of variance (ANOVA). Results were expressed as mean ± standard deviation, with P<0.05 considered significantly different. Preventive Ex impeded histological changes and increased the enzymatic defense system (SOD and GPx) by reducing oxidative damage in lipids and proteins. This preventive effect of prior physical Ex alleviates damage caused by CS exposure. |
format | Online Article Text |
id | pubmed-4809330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-48093302016-04-01 Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice Nesi, Renata Tiscoski de Souza, Priscila Soares dos Santos, Giulia Pedroso Thirupathi, Anand Menegali, Bruno T Silveira, Paulo Cesar Lock da Silva, Luciano Acordi Valença, Samuel Santos Pinho, Ricardo Aurino Int J Chron Obstruct Pulmon Dis Original Research Reactive oxygen species (ROS) are important in the pathogenesis of pulmonary injury induced by cigarette smoke (CS) exposure, and physical exercise (Ex) is useful in combating impaired oxidative process. We verified the preventive effects of Ex on lung oxidative markers induced by smoking. In this study, 36 mice (C57BL-6, 30–35 g) were split into four groups: control, CS, Ex, and CS plus Ex. Ex groups were given prior physical training in water (2×30 min/d, 5 days/wk, 8 weeks). After training, the CS groups were subjected to passive exposure to four cigarettes, 3 × per day, for 60 consecutive days. After 24 hours from the last exposure, CS animals were sacrificed, and lung samples were collected for further analysis. Left lung sample was prepared for histological analysis, and right lung was used for biochemical analysis (superoxide, hydroxyproline, lipid peroxidation [thiobarbituric acid reactive species], protein carbonylation [carbonyl groups formation], superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx] activities). Group comparisons were evaluated by analysis of variance (ANOVA). Results were expressed as mean ± standard deviation, with P<0.05 considered significantly different. Preventive Ex impeded histological changes and increased the enzymatic defense system (SOD and GPx) by reducing oxidative damage in lipids and proteins. This preventive effect of prior physical Ex alleviates damage caused by CS exposure. Dove Medical Press 2016-03-22 /pmc/articles/PMC4809330/ /pubmed/27042047 http://dx.doi.org/10.2147/COPD.S93958 Text en © 2016 Nesi et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Nesi, Renata Tiscoski de Souza, Priscila Soares dos Santos, Giulia Pedroso Thirupathi, Anand Menegali, Bruno T Silveira, Paulo Cesar Lock da Silva, Luciano Acordi Valença, Samuel Santos Pinho, Ricardo Aurino Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice |
title | Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice |
title_full | Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice |
title_fullStr | Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice |
title_full_unstemmed | Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice |
title_short | Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice |
title_sort | physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809330/ https://www.ncbi.nlm.nih.gov/pubmed/27042047 http://dx.doi.org/10.2147/COPD.S93958 |
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