Cargando…

Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax

BACKGROUND: Plasmodium vivax is the most widely distributed human malaria parasite with 2.9 billion people living in endemic areas. Despite intensive malaria control efforts, the proportion of cases attributed to P. vivax is increasing in many countries. Genetic analyses of the parasite population a...

Descripción completa

Detalles Bibliográficos
Autores principales: Friedrich, Lindsey R., Popovici, Jean, Kim, Saorin, Dysoley, Lek, Zimmerman, Peter A., Menard, Didier, Serre, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809505/
https://www.ncbi.nlm.nih.gov/pubmed/27018585
http://dx.doi.org/10.1371/journal.pntd.0004526
_version_ 1782423647804194816
author Friedrich, Lindsey R.
Popovici, Jean
Kim, Saorin
Dysoley, Lek
Zimmerman, Peter A.
Menard, Didier
Serre, David
author_facet Friedrich, Lindsey R.
Popovici, Jean
Kim, Saorin
Dysoley, Lek
Zimmerman, Peter A.
Menard, Didier
Serre, David
author_sort Friedrich, Lindsey R.
collection PubMed
description BACKGROUND: Plasmodium vivax is the most widely distributed human malaria parasite with 2.9 billion people living in endemic areas. Despite intensive malaria control efforts, the proportion of cases attributed to P. vivax is increasing in many countries. Genetic analyses of the parasite population and its dynamics could provide an assessment of the efficacy of control efforts, but, unfortunately, these studies are limited in P. vivax by the lack of informative markers and high-throughput genotyping methods. METHODOLOGY/PRINCIPAL FINDINGS: We developed a sequencing-based assay to simultaneously genotype more than 100 SNPs and applied this approach to ~500 P. vivax-infected individuals recruited across nine locations in Cambodia between 2004 and 2013. Our analyses showed that the vast majority of infections are polyclonal (92%) and that P. vivax displays high genetic diversity in Cambodia without apparent geographic stratification. Interestingly, our analyses also revealed that the proportion of monoclonal infections significantly increased between 2004 and 2013, possibly suggesting that malaria control strategies in Cambodia may be successfully affecting the parasite population. CONCLUSIONS/SIGNIFICANCE: Our findings demonstrate that this high-throughput genotyping assay is efficient in characterizing P. vivax diversity and can provide valuable insights to assess the efficacy of malaria elimination programs or to monitor the spread of specific parasites.
format Online
Article
Text
id pubmed-4809505
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-48095052016-04-05 Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax Friedrich, Lindsey R. Popovici, Jean Kim, Saorin Dysoley, Lek Zimmerman, Peter A. Menard, Didier Serre, David PLoS Negl Trop Dis Research Article BACKGROUND: Plasmodium vivax is the most widely distributed human malaria parasite with 2.9 billion people living in endemic areas. Despite intensive malaria control efforts, the proportion of cases attributed to P. vivax is increasing in many countries. Genetic analyses of the parasite population and its dynamics could provide an assessment of the efficacy of control efforts, but, unfortunately, these studies are limited in P. vivax by the lack of informative markers and high-throughput genotyping methods. METHODOLOGY/PRINCIPAL FINDINGS: We developed a sequencing-based assay to simultaneously genotype more than 100 SNPs and applied this approach to ~500 P. vivax-infected individuals recruited across nine locations in Cambodia between 2004 and 2013. Our analyses showed that the vast majority of infections are polyclonal (92%) and that P. vivax displays high genetic diversity in Cambodia without apparent geographic stratification. Interestingly, our analyses also revealed that the proportion of monoclonal infections significantly increased between 2004 and 2013, possibly suggesting that malaria control strategies in Cambodia may be successfully affecting the parasite population. CONCLUSIONS/SIGNIFICANCE: Our findings demonstrate that this high-throughput genotyping assay is efficient in characterizing P. vivax diversity and can provide valuable insights to assess the efficacy of malaria elimination programs or to monitor the spread of specific parasites. Public Library of Science 2016-03-28 /pmc/articles/PMC4809505/ /pubmed/27018585 http://dx.doi.org/10.1371/journal.pntd.0004526 Text en © 2016 Friedrich et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Friedrich, Lindsey R.
Popovici, Jean
Kim, Saorin
Dysoley, Lek
Zimmerman, Peter A.
Menard, Didier
Serre, David
Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax
title Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax
title_full Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax
title_fullStr Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax
title_full_unstemmed Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax
title_short Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax
title_sort complexity of infection and genetic diversity in cambodian plasmodium vivax
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809505/
https://www.ncbi.nlm.nih.gov/pubmed/27018585
http://dx.doi.org/10.1371/journal.pntd.0004526
work_keys_str_mv AT friedrichlindseyr complexityofinfectionandgeneticdiversityincambodianplasmodiumvivax
AT popovicijean complexityofinfectionandgeneticdiversityincambodianplasmodiumvivax
AT kimsaorin complexityofinfectionandgeneticdiversityincambodianplasmodiumvivax
AT dysoleylek complexityofinfectionandgeneticdiversityincambodianplasmodiumvivax
AT zimmermanpetera complexityofinfectionandgeneticdiversityincambodianplasmodiumvivax
AT menarddidier complexityofinfectionandgeneticdiversityincambodianplasmodiumvivax
AT serredavid complexityofinfectionandgeneticdiversityincambodianplasmodiumvivax