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Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus
We have developed a simple and sensitive method for the detection of influenza A virus based on giant magnetoresistance (GMR) biosensor. This assay employs monoclonal antibodies to viral nucleoprotein (NP) in combination with magnetic nanoparticles (MNPs). Presence of influenza virus allows the bind...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809872/ https://www.ncbi.nlm.nih.gov/pubmed/27065967 http://dx.doi.org/10.3389/fmicb.2016.00400 |
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author | Krishna, Venkatramana D. Wu, Kai Perez, Andres M. Wang, Jian-Ping |
author_facet | Krishna, Venkatramana D. Wu, Kai Perez, Andres M. Wang, Jian-Ping |
author_sort | Krishna, Venkatramana D. |
collection | PubMed |
description | We have developed a simple and sensitive method for the detection of influenza A virus based on giant magnetoresistance (GMR) biosensor. This assay employs monoclonal antibodies to viral nucleoprotein (NP) in combination with magnetic nanoparticles (MNPs). Presence of influenza virus allows the binding of MNPs to the GMR sensor and the binding is proportional to the concentration of virus. Binding of MNPs onto the GMR sensor causes change in the resistance of sensor, which is measured in a real time electrical readout. GMR biosensor detected as low as 1.5 × 10(2) TCID(50)/mL virus and the signal intensity increased with increasing concentration of virus up to 1.0 × 10(5) TCID(50)/mL. This study showed that the GMR biosensor assay is relevant for diagnostic application since the virus concentration in nasal samples of influenza virus infected swine was reported to be in the range of 10(3) to 10(5) TCID(50)/mL. |
format | Online Article Text |
id | pubmed-4809872 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-48098722016-04-08 Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus Krishna, Venkatramana D. Wu, Kai Perez, Andres M. Wang, Jian-Ping Front Microbiol Microbiology We have developed a simple and sensitive method for the detection of influenza A virus based on giant magnetoresistance (GMR) biosensor. This assay employs monoclonal antibodies to viral nucleoprotein (NP) in combination with magnetic nanoparticles (MNPs). Presence of influenza virus allows the binding of MNPs to the GMR sensor and the binding is proportional to the concentration of virus. Binding of MNPs onto the GMR sensor causes change in the resistance of sensor, which is measured in a real time electrical readout. GMR biosensor detected as low as 1.5 × 10(2) TCID(50)/mL virus and the signal intensity increased with increasing concentration of virus up to 1.0 × 10(5) TCID(50)/mL. This study showed that the GMR biosensor assay is relevant for diagnostic application since the virus concentration in nasal samples of influenza virus infected swine was reported to be in the range of 10(3) to 10(5) TCID(50)/mL. Frontiers Media S.A. 2016-03-29 /pmc/articles/PMC4809872/ /pubmed/27065967 http://dx.doi.org/10.3389/fmicb.2016.00400 Text en Copyright © 2016 Krishna, Wu, Perez and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Krishna, Venkatramana D. Wu, Kai Perez, Andres M. Wang, Jian-Ping Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus |
title | Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus |
title_full | Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus |
title_fullStr | Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus |
title_full_unstemmed | Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus |
title_short | Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus |
title_sort | giant magnetoresistance-based biosensor for detection of influenza a virus |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809872/ https://www.ncbi.nlm.nih.gov/pubmed/27065967 http://dx.doi.org/10.3389/fmicb.2016.00400 |
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