Development and Validation of an Improved PCR Method Using the 23S-5S Intergenic Spacer for Detection of Rickettsiae in Dermacentor variabilis Ticks and Tissue Samples from Humans and Laboratory Animals

A novel nested PCR assay was developed to detect Rickettsia spp. in ticks and tissue samples from humans and laboratory animals. Primers were designed for the nested run to amplify a variable region of the 23S-5S intergenic spacer (IGS) of Rickettsia spp. The newly designed primers were evaluated us...

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Detalles Bibliográficos
Autores principales: Kakumanu, Madhavi L., Ponnusamy, Loganathan, Sutton, Haley T., Meshnick, Steven R., Nicholson, William L., Apperson, Charles S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Clinical Veterinary Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809929/
https://www.ncbi.nlm.nih.gov/pubmed/26818674
http://dx.doi.org/10.1128/JCM.02605-15

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809929/
https://www.ncbi.nlm.nih.gov/pubmed/26818674
http://dx.doi.org/10.1128/JCM.02605-15

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