Cargando…
Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters
A series of insecticidal dihydroagarofuran sesquiterpene polyesters were isolated from the root bark of Chinese bittersweet (Celastrus angulatus Max). A previous study indicated that these compounds affect the digestive system of insects, and aminopeptidase N3 and V-ATPase have been identified as th...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810224/ https://www.ncbi.nlm.nih.gov/pubmed/26999207 http://dx.doi.org/10.3390/toxins8030079 |
_version_ | 1782423755109171200 |
---|---|
author | Lu, Lina Qi, Zhijun Li, Qiuli Wu, Wenjun |
author_facet | Lu, Lina Qi, Zhijun Li, Qiuli Wu, Wenjun |
author_sort | Lu, Lina |
collection | PubMed |
description | A series of insecticidal dihydroagarofuran sesquiterpene polyesters were isolated from the root bark of Chinese bittersweet (Celastrus angulatus Max). A previous study indicated that these compounds affect the digestive system of insects, and aminopeptidase N3 and V-ATPase have been identified as the most putative target proteins by affinity chromatography. In this study, the correlation between the affinity of the compounds to subunit H and the insecticidal activity or inhibitory effect on the activity of V-ATPase was analyzed to validate the target protein. Results indicated that the subunit H of V-ATPase was the target protein of the insecticidal compounds. In addition, the possible mechanism of action of the compounds was discussed. The results provide new ideas for developing pesticides acting on V-ATPase of insects. |
format | Online Article Text |
id | pubmed-4810224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-48102242016-04-04 Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters Lu, Lina Qi, Zhijun Li, Qiuli Wu, Wenjun Toxins (Basel) Article A series of insecticidal dihydroagarofuran sesquiterpene polyesters were isolated from the root bark of Chinese bittersweet (Celastrus angulatus Max). A previous study indicated that these compounds affect the digestive system of insects, and aminopeptidase N3 and V-ATPase have been identified as the most putative target proteins by affinity chromatography. In this study, the correlation between the affinity of the compounds to subunit H and the insecticidal activity or inhibitory effect on the activity of V-ATPase was analyzed to validate the target protein. Results indicated that the subunit H of V-ATPase was the target protein of the insecticidal compounds. In addition, the possible mechanism of action of the compounds was discussed. The results provide new ideas for developing pesticides acting on V-ATPase of insects. MDPI 2016-03-18 /pmc/articles/PMC4810224/ /pubmed/26999207 http://dx.doi.org/10.3390/toxins8030079 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lu, Lina Qi, Zhijun Li, Qiuli Wu, Wenjun Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters |
title | Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters |
title_full | Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters |
title_fullStr | Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters |
title_full_unstemmed | Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters |
title_short | Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters |
title_sort | validation of the target protein of insecticidal dihydroagarofuran sesquiterpene polyesters |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810224/ https://www.ncbi.nlm.nih.gov/pubmed/26999207 http://dx.doi.org/10.3390/toxins8030079 |
work_keys_str_mv | AT lulina validationofthetargetproteinofinsecticidaldihydroagarofuransesquiterpenepolyesters AT qizhijun validationofthetargetproteinofinsecticidaldihydroagarofuransesquiterpenepolyesters AT liqiuli validationofthetargetproteinofinsecticidaldihydroagarofuransesquiterpenepolyesters AT wuwenjun validationofthetargetproteinofinsecticidaldihydroagarofuransesquiterpenepolyesters |