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Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters

A series of insecticidal dihydroagarofuran sesquiterpene polyesters were isolated from the root bark of Chinese bittersweet (Celastrus angulatus Max). A previous study indicated that these compounds affect the digestive system of insects, and aminopeptidase N3 and V-ATPase have been identified as th...

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Detalles Bibliográficos
Autores principales: Lu, Lina, Qi, Zhijun, Li, Qiuli, Wu, Wenjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810224/
https://www.ncbi.nlm.nih.gov/pubmed/26999207
http://dx.doi.org/10.3390/toxins8030079
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author Lu, Lina
Qi, Zhijun
Li, Qiuli
Wu, Wenjun
author_facet Lu, Lina
Qi, Zhijun
Li, Qiuli
Wu, Wenjun
author_sort Lu, Lina
collection PubMed
description A series of insecticidal dihydroagarofuran sesquiterpene polyesters were isolated from the root bark of Chinese bittersweet (Celastrus angulatus Max). A previous study indicated that these compounds affect the digestive system of insects, and aminopeptidase N3 and V-ATPase have been identified as the most putative target proteins by affinity chromatography. In this study, the correlation between the affinity of the compounds to subunit H and the insecticidal activity or inhibitory effect on the activity of V-ATPase was analyzed to validate the target protein. Results indicated that the subunit H of V-ATPase was the target protein of the insecticidal compounds. In addition, the possible mechanism of action of the compounds was discussed. The results provide new ideas for developing pesticides acting on V-ATPase of insects.
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spelling pubmed-48102242016-04-04 Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters Lu, Lina Qi, Zhijun Li, Qiuli Wu, Wenjun Toxins (Basel) Article A series of insecticidal dihydroagarofuran sesquiterpene polyesters were isolated from the root bark of Chinese bittersweet (Celastrus angulatus Max). A previous study indicated that these compounds affect the digestive system of insects, and aminopeptidase N3 and V-ATPase have been identified as the most putative target proteins by affinity chromatography. In this study, the correlation between the affinity of the compounds to subunit H and the insecticidal activity or inhibitory effect on the activity of V-ATPase was analyzed to validate the target protein. Results indicated that the subunit H of V-ATPase was the target protein of the insecticidal compounds. In addition, the possible mechanism of action of the compounds was discussed. The results provide new ideas for developing pesticides acting on V-ATPase of insects. MDPI 2016-03-18 /pmc/articles/PMC4810224/ /pubmed/26999207 http://dx.doi.org/10.3390/toxins8030079 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Lina
Qi, Zhijun
Li, Qiuli
Wu, Wenjun
Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters
title Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters
title_full Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters
title_fullStr Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters
title_full_unstemmed Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters
title_short Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters
title_sort validation of the target protein of insecticidal dihydroagarofuran sesquiterpene polyesters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810224/
https://www.ncbi.nlm.nih.gov/pubmed/26999207
http://dx.doi.org/10.3390/toxins8030079
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