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Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore

Accurate chromosome segregation depends on proper kinetochore–microtubule attachment. Upon microtubule interaction, kinetochores are subjected to forces generated by the microtubules. In this work, we used laser ablation to sever microtubules attached to a merotelic kinetochore, which is laterally s...

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Autores principales: Cojoc, Gheorghe, Roscioli, Emanuele, Zhang, Lijuan, García-Ulloa, Alfonso, Shah, Jagesh V., Berns, Michael W., Pavin, Nenad, Cimini, Daniela, Tolić, Iva M., Gregan, Juraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810299/
https://www.ncbi.nlm.nih.gov/pubmed/27002163
http://dx.doi.org/10.1083/jcb.201506011
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author Cojoc, Gheorghe
Roscioli, Emanuele
Zhang, Lijuan
García-Ulloa, Alfonso
Shah, Jagesh V.
Berns, Michael W.
Pavin, Nenad
Cimini, Daniela
Tolić, Iva M.
Gregan, Juraj
author_facet Cojoc, Gheorghe
Roscioli, Emanuele
Zhang, Lijuan
García-Ulloa, Alfonso
Shah, Jagesh V.
Berns, Michael W.
Pavin, Nenad
Cimini, Daniela
Tolić, Iva M.
Gregan, Juraj
author_sort Cojoc, Gheorghe
collection PubMed
description Accurate chromosome segregation depends on proper kinetochore–microtubule attachment. Upon microtubule interaction, kinetochores are subjected to forces generated by the microtubules. In this work, we used laser ablation to sever microtubules attached to a merotelic kinetochore, which is laterally stretched by opposing pulling forces exerted by microtubules, and inferred the mechanical response of the kinetochore from its length change. In both mammalian PtK1 cells and in the fission yeast Schizosaccharomyces pombe, kinetochores shortened after microtubule severing. Interestingly, the inner kinetochore–centromere relaxed faster than the outer kinetochore. Whereas in fission yeast all kinetochores relaxed to a similar length, in PtK1 cells the more stretched kinetochores remained more stretched. Simple models suggest that these differences arise because the mechanical structure of the mammalian kinetochore is more complex. Our study establishes merotelic kinetochores as an experimental model for studying the mechanical response of the kinetochore in live cells and reveals a viscoelastic behavior of the kinetochore that is conserved in yeast and mammalian cells.
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spelling pubmed-48102992016-09-28 Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore Cojoc, Gheorghe Roscioli, Emanuele Zhang, Lijuan García-Ulloa, Alfonso Shah, Jagesh V. Berns, Michael W. Pavin, Nenad Cimini, Daniela Tolić, Iva M. Gregan, Juraj J Cell Biol Research Articles Accurate chromosome segregation depends on proper kinetochore–microtubule attachment. Upon microtubule interaction, kinetochores are subjected to forces generated by the microtubules. In this work, we used laser ablation to sever microtubules attached to a merotelic kinetochore, which is laterally stretched by opposing pulling forces exerted by microtubules, and inferred the mechanical response of the kinetochore from its length change. In both mammalian PtK1 cells and in the fission yeast Schizosaccharomyces pombe, kinetochores shortened after microtubule severing. Interestingly, the inner kinetochore–centromere relaxed faster than the outer kinetochore. Whereas in fission yeast all kinetochores relaxed to a similar length, in PtK1 cells the more stretched kinetochores remained more stretched. Simple models suggest that these differences arise because the mechanical structure of the mammalian kinetochore is more complex. Our study establishes merotelic kinetochores as an experimental model for studying the mechanical response of the kinetochore in live cells and reveals a viscoelastic behavior of the kinetochore that is conserved in yeast and mammalian cells. The Rockefeller University Press 2016-03-28 /pmc/articles/PMC4810299/ /pubmed/27002163 http://dx.doi.org/10.1083/jcb.201506011 Text en © 2016 Cojoc et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Cojoc, Gheorghe
Roscioli, Emanuele
Zhang, Lijuan
García-Ulloa, Alfonso
Shah, Jagesh V.
Berns, Michael W.
Pavin, Nenad
Cimini, Daniela
Tolić, Iva M.
Gregan, Juraj
Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore
title Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore
title_full Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore
title_fullStr Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore
title_full_unstemmed Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore
title_short Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore
title_sort laser microsurgery reveals conserved viscoelastic behavior of the kinetochore
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810299/
https://www.ncbi.nlm.nih.gov/pubmed/27002163
http://dx.doi.org/10.1083/jcb.201506011
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