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Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo
Increasing evidence implicates Ca(2+) in the control of cell migration. However, the underlying mechanisms are incompletely understood. Acidic Ca(2+) stores are fast emerging as signaling centers. But how Ca(2+) is taken up by these organelles in metazoans and the physiological relevance for migrati...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810305/ https://www.ncbi.nlm.nih.gov/pubmed/27002171 http://dx.doi.org/10.1083/jcb.201510019 |
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author | Melchionda, Manuela Pittman, Jon K. Mayor, Roberto Patel, Sandip |
author_facet | Melchionda, Manuela Pittman, Jon K. Mayor, Roberto Patel, Sandip |
author_sort | Melchionda, Manuela |
collection | PubMed |
description | Increasing evidence implicates Ca(2+) in the control of cell migration. However, the underlying mechanisms are incompletely understood. Acidic Ca(2+) stores are fast emerging as signaling centers. But how Ca(2+) is taken up by these organelles in metazoans and the physiological relevance for migration is unclear. Here, we identify a vertebrate Ca(2+)/H(+) exchanger (CAX) as part of a widespread family of homologues in animals. CAX is expressed in neural crest cells and required for their migration in vivo. It localizes to acidic organelles, tempers evoked Ca(2+) signals, and regulates cell-matrix adhesion during migration. Our data provide new molecular insight into how Ca(2+) is handled by acidic organelles and link this to migration, thereby underscoring the role of noncanonical Ca(2+) stores in the control of Ca(2+)-dependent function. |
format | Online Article Text |
id | pubmed-4810305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-48103052016-09-28 Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo Melchionda, Manuela Pittman, Jon K. Mayor, Roberto Patel, Sandip J Cell Biol Research Articles Increasing evidence implicates Ca(2+) in the control of cell migration. However, the underlying mechanisms are incompletely understood. Acidic Ca(2+) stores are fast emerging as signaling centers. But how Ca(2+) is taken up by these organelles in metazoans and the physiological relevance for migration is unclear. Here, we identify a vertebrate Ca(2+)/H(+) exchanger (CAX) as part of a widespread family of homologues in animals. CAX is expressed in neural crest cells and required for their migration in vivo. It localizes to acidic organelles, tempers evoked Ca(2+) signals, and regulates cell-matrix adhesion during migration. Our data provide new molecular insight into how Ca(2+) is handled by acidic organelles and link this to migration, thereby underscoring the role of noncanonical Ca(2+) stores in the control of Ca(2+)-dependent function. The Rockefeller University Press 2016-03-28 /pmc/articles/PMC4810305/ /pubmed/27002171 http://dx.doi.org/10.1083/jcb.201510019 Text en © 2016 Melchionda et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Melchionda, Manuela Pittman, Jon K. Mayor, Roberto Patel, Sandip Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo |
title | Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo |
title_full | Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo |
title_fullStr | Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo |
title_full_unstemmed | Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo |
title_short | Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo |
title_sort | ca(2+)/h(+) exchange by acidic organelles regulates cell migration in vivo |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810305/ https://www.ncbi.nlm.nih.gov/pubmed/27002171 http://dx.doi.org/10.1083/jcb.201510019 |
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