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Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo

Increasing evidence implicates Ca(2+) in the control of cell migration. However, the underlying mechanisms are incompletely understood. Acidic Ca(2+) stores are fast emerging as signaling centers. But how Ca(2+) is taken up by these organelles in metazoans and the physiological relevance for migrati...

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Detalles Bibliográficos
Autores principales: Melchionda, Manuela, Pittman, Jon K., Mayor, Roberto, Patel, Sandip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810305/
https://www.ncbi.nlm.nih.gov/pubmed/27002171
http://dx.doi.org/10.1083/jcb.201510019
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author Melchionda, Manuela
Pittman, Jon K.
Mayor, Roberto
Patel, Sandip
author_facet Melchionda, Manuela
Pittman, Jon K.
Mayor, Roberto
Patel, Sandip
author_sort Melchionda, Manuela
collection PubMed
description Increasing evidence implicates Ca(2+) in the control of cell migration. However, the underlying mechanisms are incompletely understood. Acidic Ca(2+) stores are fast emerging as signaling centers. But how Ca(2+) is taken up by these organelles in metazoans and the physiological relevance for migration is unclear. Here, we identify a vertebrate Ca(2+)/H(+) exchanger (CAX) as part of a widespread family of homologues in animals. CAX is expressed in neural crest cells and required for their migration in vivo. It localizes to acidic organelles, tempers evoked Ca(2+) signals, and regulates cell-matrix adhesion during migration. Our data provide new molecular insight into how Ca(2+) is handled by acidic organelles and link this to migration, thereby underscoring the role of noncanonical Ca(2+) stores in the control of Ca(2+)-dependent function.
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spelling pubmed-48103052016-09-28 Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo Melchionda, Manuela Pittman, Jon K. Mayor, Roberto Patel, Sandip J Cell Biol Research Articles Increasing evidence implicates Ca(2+) in the control of cell migration. However, the underlying mechanisms are incompletely understood. Acidic Ca(2+) stores are fast emerging as signaling centers. But how Ca(2+) is taken up by these organelles in metazoans and the physiological relevance for migration is unclear. Here, we identify a vertebrate Ca(2+)/H(+) exchanger (CAX) as part of a widespread family of homologues in animals. CAX is expressed in neural crest cells and required for their migration in vivo. It localizes to acidic organelles, tempers evoked Ca(2+) signals, and regulates cell-matrix adhesion during migration. Our data provide new molecular insight into how Ca(2+) is handled by acidic organelles and link this to migration, thereby underscoring the role of noncanonical Ca(2+) stores in the control of Ca(2+)-dependent function. The Rockefeller University Press 2016-03-28 /pmc/articles/PMC4810305/ /pubmed/27002171 http://dx.doi.org/10.1083/jcb.201510019 Text en © 2016 Melchionda et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Melchionda, Manuela
Pittman, Jon K.
Mayor, Roberto
Patel, Sandip
Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo
title Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo
title_full Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo
title_fullStr Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo
title_full_unstemmed Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo
title_short Ca(2+)/H(+) exchange by acidic organelles regulates cell migration in vivo
title_sort ca(2+)/h(+) exchange by acidic organelles regulates cell migration in vivo
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810305/
https://www.ncbi.nlm.nih.gov/pubmed/27002171
http://dx.doi.org/10.1083/jcb.201510019
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