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TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5)
GTPase of the immune associated nucleotide binding protein (GIMAP) family of proteins are expressed essentially in cells of the hematopoietic system. Mutation in the founding member of this gene family, Gimap5, results in the lymphopenic phenotype in Bio-Breeding diabetes prone rats. In mice, deleti...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811415/ https://www.ncbi.nlm.nih.gov/pubmed/27023180 http://dx.doi.org/10.1371/journal.pone.0151837 |
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author | Chen, Xi-Lin Serrano, Daniel Ghobadi, Farnaz Mayhue, Marian Hoebe, Kasper Ilangumaran, Subburaj Ramanathan, Sheela |
author_facet | Chen, Xi-Lin Serrano, Daniel Ghobadi, Farnaz Mayhue, Marian Hoebe, Kasper Ilangumaran, Subburaj Ramanathan, Sheela |
author_sort | Chen, Xi-Lin |
collection | PubMed |
description | GTPase of the immune associated nucleotide binding protein (GIMAP) family of proteins are expressed essentially in cells of the hematopoietic system. Mutation in the founding member of this gene family, Gimap5, results in the lymphopenic phenotype in Bio-Breeding diabetes prone rats. In mice, deletion of functional Gimap5 gene affects the survival and renewal of hematopoietic stem cells in addition to the defects observed in T cells. Here we show that T cells from OTII TCR-transgenic Gimap5(sph/sph) mice do not proliferate in response to its cognate antigen. Furthermore, T cells from Gimap5 mutant rats and mice show decreased phosphorylation of STAT5 following stimulation with IL-7. Our results suggest that functional Gimap5 is required for optimal signaling through TCR and IL-7R in T cells. |
format | Online Article Text |
id | pubmed-4811415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48114152016-04-05 TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5) Chen, Xi-Lin Serrano, Daniel Ghobadi, Farnaz Mayhue, Marian Hoebe, Kasper Ilangumaran, Subburaj Ramanathan, Sheela PLoS One Research Article GTPase of the immune associated nucleotide binding protein (GIMAP) family of proteins are expressed essentially in cells of the hematopoietic system. Mutation in the founding member of this gene family, Gimap5, results in the lymphopenic phenotype in Bio-Breeding diabetes prone rats. In mice, deletion of functional Gimap5 gene affects the survival and renewal of hematopoietic stem cells in addition to the defects observed in T cells. Here we show that T cells from OTII TCR-transgenic Gimap5(sph/sph) mice do not proliferate in response to its cognate antigen. Furthermore, T cells from Gimap5 mutant rats and mice show decreased phosphorylation of STAT5 following stimulation with IL-7. Our results suggest that functional Gimap5 is required for optimal signaling through TCR and IL-7R in T cells. Public Library of Science 2016-03-29 /pmc/articles/PMC4811415/ /pubmed/27023180 http://dx.doi.org/10.1371/journal.pone.0151837 Text en © 2016 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Chen, Xi-Lin Serrano, Daniel Ghobadi, Farnaz Mayhue, Marian Hoebe, Kasper Ilangumaran, Subburaj Ramanathan, Sheela TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5) |
title | TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5) |
title_full | TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5) |
title_fullStr | TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5) |
title_full_unstemmed | TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5) |
title_short | TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5) |
title_sort | tcr and il-7 signaling are altered in the absence of functional gtpase of the immune associated nucleotide binding protein 5 (gimap5) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811415/ https://www.ncbi.nlm.nih.gov/pubmed/27023180 http://dx.doi.org/10.1371/journal.pone.0151837 |
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