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microRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer
OBJECTIVE: Detection of microRNA (miRNA) aberrations in human faeces is a new approach for colorectal cancer (CRC) screening. The aim of this study was to characterise miR-20a in faeces as a non-invasive biomarker for diagnosis of CRC. RESULTS: miR-20a expression was significantly higher in the 40 C...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811480/ https://www.ncbi.nlm.nih.gov/pubmed/26621842 |
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author | Yau, Tung On Wu, Chung Wah Tang, Ceen-Ming Chen, Yingxuan Fang, Jingyuan Dong, Yujuan Liang, Qiaoyi Man Ng, Simon Siu Chan, Francis Ka Leung Sung, Joseph Jao Yiu Yu, Jun |
author_facet | Yau, Tung On Wu, Chung Wah Tang, Ceen-Ming Chen, Yingxuan Fang, Jingyuan Dong, Yujuan Liang, Qiaoyi Man Ng, Simon Siu Chan, Francis Ka Leung Sung, Joseph Jao Yiu Yu, Jun |
author_sort | Yau, Tung On |
collection | PubMed |
description | OBJECTIVE: Detection of microRNA (miRNA) aberrations in human faeces is a new approach for colorectal cancer (CRC) screening. The aim of this study was to characterise miR-20a in faeces as a non-invasive biomarker for diagnosis of CRC. RESULTS: miR-20a expression was significantly higher in the 40 CRC tumours compared to their respective adjacent normal tissues (P = 0.0065). Levels of miR-20a were also significantly higher in faecal samples from CRC patients (P < 0.0001). The area under receiver operating characteristic (AUROC) curve for miR-20a was 0.73, with a sensitivity of 55% and specificity of 82% for CRC patients compared with controls. No significant difference in the level of miR-20a was found between patients with proximal, distal, and rectal cancer. The use of antibiotics did not influence faecal miR-20a levels. PATIENTS AND METHODS: miR-20a was selected from an expression microarray containing 667 miRNAs. Further verification of miR-20a was performed in 40 pairs of primary CRC tissues, as well as 595 faecal samples (198 CRCs, 199 adenomas, and 198 healthy controls) using TaqMan probe based quantitative Real-Time PCR (qRT-PCR). CONCLUSIONS: Faecal-based miR-20a can be utilised as a potential non-invasive biomarker for CRC screening. |
format | Online Article Text |
id | pubmed-4811480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-48114802016-04-25 microRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer Yau, Tung On Wu, Chung Wah Tang, Ceen-Ming Chen, Yingxuan Fang, Jingyuan Dong, Yujuan Liang, Qiaoyi Man Ng, Simon Siu Chan, Francis Ka Leung Sung, Joseph Jao Yiu Yu, Jun Oncotarget Research Paper OBJECTIVE: Detection of microRNA (miRNA) aberrations in human faeces is a new approach for colorectal cancer (CRC) screening. The aim of this study was to characterise miR-20a in faeces as a non-invasive biomarker for diagnosis of CRC. RESULTS: miR-20a expression was significantly higher in the 40 CRC tumours compared to their respective adjacent normal tissues (P = 0.0065). Levels of miR-20a were also significantly higher in faecal samples from CRC patients (P < 0.0001). The area under receiver operating characteristic (AUROC) curve for miR-20a was 0.73, with a sensitivity of 55% and specificity of 82% for CRC patients compared with controls. No significant difference in the level of miR-20a was found between patients with proximal, distal, and rectal cancer. The use of antibiotics did not influence faecal miR-20a levels. PATIENTS AND METHODS: miR-20a was selected from an expression microarray containing 667 miRNAs. Further verification of miR-20a was performed in 40 pairs of primary CRC tissues, as well as 595 faecal samples (198 CRCs, 199 adenomas, and 198 healthy controls) using TaqMan probe based quantitative Real-Time PCR (qRT-PCR). CONCLUSIONS: Faecal-based miR-20a can be utilised as a potential non-invasive biomarker for CRC screening. Impact Journals LLC 2015-11-26 /pmc/articles/PMC4811480/ /pubmed/26621842 Text en Copyright: © 2016 Yau et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Yau, Tung On Wu, Chung Wah Tang, Ceen-Ming Chen, Yingxuan Fang, Jingyuan Dong, Yujuan Liang, Qiaoyi Man Ng, Simon Siu Chan, Francis Ka Leung Sung, Joseph Jao Yiu Yu, Jun microRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer |
title | microRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer |
title_full | microRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer |
title_fullStr | microRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer |
title_full_unstemmed | microRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer |
title_short | microRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer |
title_sort | microrna-20a in human faeces as a non-invasive biomarker for colorectal cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811480/ https://www.ncbi.nlm.nih.gov/pubmed/26621842 |
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