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The Plasmodium PHIST and RESA-Like Protein Families of Human and Rodent Malaria Parasites
The phist gene family has members identified across the Plasmodium genus, defined by the presence of a domain of roughly 150 amino acids having conserved aromatic residues and an all alpha-helical structure. The family is highly amplified in P. falciparum, with 65 predicted genes in the genome of th...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811531/ https://www.ncbi.nlm.nih.gov/pubmed/27022937 http://dx.doi.org/10.1371/journal.pone.0152510 |
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author | Moreira, Cristina K. Naissant, Bernina Coppi, Alida Bennett, Brandy L. Aime, Elena Franke-Fayard, Blandine Janse, Chris J. Coppens, Isabelle Sinnis, Photini Templeton, Thomas J. |
author_facet | Moreira, Cristina K. Naissant, Bernina Coppi, Alida Bennett, Brandy L. Aime, Elena Franke-Fayard, Blandine Janse, Chris J. Coppens, Isabelle Sinnis, Photini Templeton, Thomas J. |
author_sort | Moreira, Cristina K. |
collection | PubMed |
description | The phist gene family has members identified across the Plasmodium genus, defined by the presence of a domain of roughly 150 amino acids having conserved aromatic residues and an all alpha-helical structure. The family is highly amplified in P. falciparum, with 65 predicted genes in the genome of the 3D7 isolate. In contrast, in the rodent malaria parasite P. berghei 3 genes are identified, one of which is an apparent pseudogene. Transcripts of the P. berghei phist genes are predominant in schizonts, whereas in P. falciparum transcript profiles span different asexual blood stages and gametocytes. We pursued targeted disruption of P. berghei phist genes in order to characterize a simplistic model for the expanded phist gene repertoire in P. falciparum. Unsuccessful attempts to disrupt P. berghei PBANKA_114540 suggest that this phist gene is essential, while knockout of phist PBANKA_122900 shows an apparent normal progression and non-essential function throughout the life cycle. Epitope-tagging of P. falciparum and P. berghei phist genes confirmed protein export to the erythrocyte cytoplasm and localization with a punctate pattern. Three P. berghei PEXEL/HT-positive exported proteins exhibit at least partial co-localization, in support of a common vesicular compartment in the cytoplasm of erythrocytes infected with rodent malaria parasites. |
format | Online Article Text |
id | pubmed-4811531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48115312016-04-05 The Plasmodium PHIST and RESA-Like Protein Families of Human and Rodent Malaria Parasites Moreira, Cristina K. Naissant, Bernina Coppi, Alida Bennett, Brandy L. Aime, Elena Franke-Fayard, Blandine Janse, Chris J. Coppens, Isabelle Sinnis, Photini Templeton, Thomas J. PLoS One Research Article The phist gene family has members identified across the Plasmodium genus, defined by the presence of a domain of roughly 150 amino acids having conserved aromatic residues and an all alpha-helical structure. The family is highly amplified in P. falciparum, with 65 predicted genes in the genome of the 3D7 isolate. In contrast, in the rodent malaria parasite P. berghei 3 genes are identified, one of which is an apparent pseudogene. Transcripts of the P. berghei phist genes are predominant in schizonts, whereas in P. falciparum transcript profiles span different asexual blood stages and gametocytes. We pursued targeted disruption of P. berghei phist genes in order to characterize a simplistic model for the expanded phist gene repertoire in P. falciparum. Unsuccessful attempts to disrupt P. berghei PBANKA_114540 suggest that this phist gene is essential, while knockout of phist PBANKA_122900 shows an apparent normal progression and non-essential function throughout the life cycle. Epitope-tagging of P. falciparum and P. berghei phist genes confirmed protein export to the erythrocyte cytoplasm and localization with a punctate pattern. Three P. berghei PEXEL/HT-positive exported proteins exhibit at least partial co-localization, in support of a common vesicular compartment in the cytoplasm of erythrocytes infected with rodent malaria parasites. Public Library of Science 2016-03-29 /pmc/articles/PMC4811531/ /pubmed/27022937 http://dx.doi.org/10.1371/journal.pone.0152510 Text en © 2016 Moreira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Moreira, Cristina K. Naissant, Bernina Coppi, Alida Bennett, Brandy L. Aime, Elena Franke-Fayard, Blandine Janse, Chris J. Coppens, Isabelle Sinnis, Photini Templeton, Thomas J. The Plasmodium PHIST and RESA-Like Protein Families of Human and Rodent Malaria Parasites |
title | The Plasmodium PHIST and RESA-Like Protein Families of Human and Rodent Malaria Parasites |
title_full | The Plasmodium PHIST and RESA-Like Protein Families of Human and Rodent Malaria Parasites |
title_fullStr | The Plasmodium PHIST and RESA-Like Protein Families of Human and Rodent Malaria Parasites |
title_full_unstemmed | The Plasmodium PHIST and RESA-Like Protein Families of Human and Rodent Malaria Parasites |
title_short | The Plasmodium PHIST and RESA-Like Protein Families of Human and Rodent Malaria Parasites |
title_sort | plasmodium phist and resa-like protein families of human and rodent malaria parasites |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811531/ https://www.ncbi.nlm.nih.gov/pubmed/27022937 http://dx.doi.org/10.1371/journal.pone.0152510 |
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