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Kinetic Characterisation of a Single Chain Antibody against the Hormone Abscisic Acid: Comparison with Its Parental Monoclonal

A single-chain Fv fragment antibody (scFv) specific for the plant hormone abscisic acid (ABA) has been expressed in the bacterium Escherichia coli as a fusion protein. The kinetics of ABA binding have been measured using surface plasmon resonance spectrometry (BIAcore 2000) using surface and solutio...

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Detalles Bibliográficos
Autores principales: Badescu, George O., Marsh, Andrew, Smith, Timothy R., Thompson, Andrew J., Napier, Richard M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811560/
https://www.ncbi.nlm.nih.gov/pubmed/27023768
http://dx.doi.org/10.1371/journal.pone.0152148

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