Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states

To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we developed a lysine-targeted enrichable cross-linker containing a biotin tag for affinity purification, a chemical cleavage site to separate cross-linked peptides away from biotin after enrichment, and a spacer ar...

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Detalles Bibliográficos
Autores principales: Tan, Dan, Li, Qiang, Zhang, Mei-Jun, Liu, Chao, Ma, Chengying, Zhang, Pan, Ding, Yue-He, Fan, Sheng-Bo, Tao, Li, Yang, Bing, Li, Xiangke, Ma, Shoucai, Liu, Junjie, Feng, Boya, Liu, Xiaohui, Wang, Hong-Wei, He, Si-Min, Gao, Ning, Ye, Keqiong, Dong, Meng-Qiu, Lei, Xiaoguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Biophysics and Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811778/
https://www.ncbi.nlm.nih.gov/pubmed/26952210
http://dx.doi.org/10.7554/eLife.12509

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