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Regulation of Galactolipid Biosynthesis by Overexpression of the Rice MGD Gene Contributes to Enhanced Aluminum Tolerance in Tobacco

Membrane lipid alterations affect Al tolerance in plants, but little is known about the regulation of membrane lipid metabolism in response to Al stress. Transgenic tobacco (Nicotiana tabacum) overexpressing rice monogalactosyldiacylglycerol (MGDG) synthase (OsMGD) gene and wild-type tobacco plants...

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Autores principales: Zhang, Meijuan, Deng, Xiping, Yin, Lina, Qi, Lingyun, Wang, Xinyue, Wang, Shiwen, Li, Hongbing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811928/
https://www.ncbi.nlm.nih.gov/pubmed/27066017
http://dx.doi.org/10.3389/fpls.2016.00337
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author Zhang, Meijuan
Deng, Xiping
Yin, Lina
Qi, Lingyun
Wang, Xinyue
Wang, Shiwen
Li, Hongbing
author_facet Zhang, Meijuan
Deng, Xiping
Yin, Lina
Qi, Lingyun
Wang, Xinyue
Wang, Shiwen
Li, Hongbing
author_sort Zhang, Meijuan
collection PubMed
description Membrane lipid alterations affect Al tolerance in plants, but little is known about the regulation of membrane lipid metabolism in response to Al stress. Transgenic tobacco (Nicotiana tabacum) overexpressing rice monogalactosyldiacylglycerol (MGDG) synthase (OsMGD) gene and wild-type tobacco plants were exposed to AlCl(3), and the impact of Al toxicity on root growth, Al accumulation, plasma membrane integrity, lipid peroxidation and membrane lipid composition were investigated. Compared with the wild type, the transgenic plants exhibited rapid regrowth of roots after removal of Al and less damage to membrane integrity and lipid peroxidation under Al stress, meanwhile, the Al accumulation showed no difference between wild-type and transgenic plants. Lipid analysis showed that Al treatment dramatically decreased the content of MGDG and the ratio of MGDG to digalactosyldiacylglycerol (DGDG) in wild-type plants, while it was unchanged in transgenic plants. The stable of MGDG level and the ratio of MGDG/DGDG contribute to maintain the membrane stability and permeability. Moreover, Al caused a significant increase in phospholipids in wild-type plants, resulting in a high proportion of phospholipids and low proportion of galactolipids, but these proportions were unaffected in transgenic plants. The high proportion of phospholipids could contribute to a higher rate of Al(3+) binding in the membrane and thereby leads to more membrane perturbation and damage. These results show that the regulation of galactolipid biosynthesis could play an important role in maintaining membrane structure and function under Al stress.
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spelling pubmed-48119282016-04-08 Regulation of Galactolipid Biosynthesis by Overexpression of the Rice MGD Gene Contributes to Enhanced Aluminum Tolerance in Tobacco Zhang, Meijuan Deng, Xiping Yin, Lina Qi, Lingyun Wang, Xinyue Wang, Shiwen Li, Hongbing Front Plant Sci Plant Science Membrane lipid alterations affect Al tolerance in plants, but little is known about the regulation of membrane lipid metabolism in response to Al stress. Transgenic tobacco (Nicotiana tabacum) overexpressing rice monogalactosyldiacylglycerol (MGDG) synthase (OsMGD) gene and wild-type tobacco plants were exposed to AlCl(3), and the impact of Al toxicity on root growth, Al accumulation, plasma membrane integrity, lipid peroxidation and membrane lipid composition were investigated. Compared with the wild type, the transgenic plants exhibited rapid regrowth of roots after removal of Al and less damage to membrane integrity and lipid peroxidation under Al stress, meanwhile, the Al accumulation showed no difference between wild-type and transgenic plants. Lipid analysis showed that Al treatment dramatically decreased the content of MGDG and the ratio of MGDG to digalactosyldiacylglycerol (DGDG) in wild-type plants, while it was unchanged in transgenic plants. The stable of MGDG level and the ratio of MGDG/DGDG contribute to maintain the membrane stability and permeability. Moreover, Al caused a significant increase in phospholipids in wild-type plants, resulting in a high proportion of phospholipids and low proportion of galactolipids, but these proportions were unaffected in transgenic plants. The high proportion of phospholipids could contribute to a higher rate of Al(3+) binding in the membrane and thereby leads to more membrane perturbation and damage. These results show that the regulation of galactolipid biosynthesis could play an important role in maintaining membrane structure and function under Al stress. Frontiers Media S.A. 2016-03-30 /pmc/articles/PMC4811928/ /pubmed/27066017 http://dx.doi.org/10.3389/fpls.2016.00337 Text en Copyright © 2016 Zhang, Deng, Yin, Qi, Wang, Wang and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Meijuan
Deng, Xiping
Yin, Lina
Qi, Lingyun
Wang, Xinyue
Wang, Shiwen
Li, Hongbing
Regulation of Galactolipid Biosynthesis by Overexpression of the Rice MGD Gene Contributes to Enhanced Aluminum Tolerance in Tobacco
title Regulation of Galactolipid Biosynthesis by Overexpression of the Rice MGD Gene Contributes to Enhanced Aluminum Tolerance in Tobacco
title_full Regulation of Galactolipid Biosynthesis by Overexpression of the Rice MGD Gene Contributes to Enhanced Aluminum Tolerance in Tobacco
title_fullStr Regulation of Galactolipid Biosynthesis by Overexpression of the Rice MGD Gene Contributes to Enhanced Aluminum Tolerance in Tobacco
title_full_unstemmed Regulation of Galactolipid Biosynthesis by Overexpression of the Rice MGD Gene Contributes to Enhanced Aluminum Tolerance in Tobacco
title_short Regulation of Galactolipid Biosynthesis by Overexpression of the Rice MGD Gene Contributes to Enhanced Aluminum Tolerance in Tobacco
title_sort regulation of galactolipid biosynthesis by overexpression of the rice mgd gene contributes to enhanced aluminum tolerance in tobacco
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811928/
https://www.ncbi.nlm.nih.gov/pubmed/27066017
http://dx.doi.org/10.3389/fpls.2016.00337
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