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Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters
BACKGROUND: Leishmaniasis causes alterations and lesions in the genital system, which leads to azoospermia and testicular atrophy in animals during the chronic phase of the infection. The aim of this study was to reveal the kinetics of Leishmania chagasi infection in the genital system of male golde...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4812609/ https://www.ncbi.nlm.nih.gov/pubmed/27025459 http://dx.doi.org/10.1186/s40249-016-0122-0 |
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author | Quintal, Amanda P. N. Borges, Bruna C. Brígido, Paula C. Silva, Rebecca T. Notário, Ana F. Santos, Marlus A. de Souza, Maria A. Nascimento, Fernanda G. O. Mundim, Antônio V. Costa, Guilherme M. J. Vasconcelos, André B. da Silva, Claudio V. |
author_facet | Quintal, Amanda P. N. Borges, Bruna C. Brígido, Paula C. Silva, Rebecca T. Notário, Ana F. Santos, Marlus A. de Souza, Maria A. Nascimento, Fernanda G. O. Mundim, Antônio V. Costa, Guilherme M. J. Vasconcelos, André B. da Silva, Claudio V. |
author_sort | Quintal, Amanda P. N. |
collection | PubMed |
description | BACKGROUND: Leishmaniasis causes alterations and lesions in the genital system, which leads to azoospermia and testicular atrophy in animals during the chronic phase of the infection. The aim of this study was to reveal the kinetics of Leishmania chagasi infection in the genital system of male golden hamsters (Mesocricetus auratus). METHODS: Animals were intraperitoneally inoculated with amastigotes from L. chagasi. At different time points animals were euthanized and genital organs processed for histo-pathological, qPCR, cytokines and testosterone detection assays. RESULTS: Our results showed a high parasite load in testis, followed by an increase of pro-inflammatory cytokines IL1-β, TNF-α and IFN-γ, and testosterone. Subsequently, IL-4 expression was upregulated and basal parasite persistence in testis was observed using the experimental approach. CONCLUSION: Extracellular amastigotes migrated to the epididymis posing as a potential major factor of parasite persistence and venereal transmission of L. chagasi infection in hamsters. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40249-016-0122-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4812609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48126092016-03-31 Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters Quintal, Amanda P. N. Borges, Bruna C. Brígido, Paula C. Silva, Rebecca T. Notário, Ana F. Santos, Marlus A. de Souza, Maria A. Nascimento, Fernanda G. O. Mundim, Antônio V. Costa, Guilherme M. J. Vasconcelos, André B. da Silva, Claudio V. Infect Dis Poverty Short Report BACKGROUND: Leishmaniasis causes alterations and lesions in the genital system, which leads to azoospermia and testicular atrophy in animals during the chronic phase of the infection. The aim of this study was to reveal the kinetics of Leishmania chagasi infection in the genital system of male golden hamsters (Mesocricetus auratus). METHODS: Animals were intraperitoneally inoculated with amastigotes from L. chagasi. At different time points animals were euthanized and genital organs processed for histo-pathological, qPCR, cytokines and testosterone detection assays. RESULTS: Our results showed a high parasite load in testis, followed by an increase of pro-inflammatory cytokines IL1-β, TNF-α and IFN-γ, and testosterone. Subsequently, IL-4 expression was upregulated and basal parasite persistence in testis was observed using the experimental approach. CONCLUSION: Extracellular amastigotes migrated to the epididymis posing as a potential major factor of parasite persistence and venereal transmission of L. chagasi infection in hamsters. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40249-016-0122-0) contains supplementary material, which is available to authorized users. BioMed Central 2016-03-29 /pmc/articles/PMC4812609/ /pubmed/27025459 http://dx.doi.org/10.1186/s40249-016-0122-0 Text en © Quintal et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Quintal, Amanda P. N. Borges, Bruna C. Brígido, Paula C. Silva, Rebecca T. Notário, Ana F. Santos, Marlus A. de Souza, Maria A. Nascimento, Fernanda G. O. Mundim, Antônio V. Costa, Guilherme M. J. Vasconcelos, André B. da Silva, Claudio V. Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters |
title | Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters |
title_full | Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters |
title_fullStr | Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters |
title_full_unstemmed | Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters |
title_short | Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters |
title_sort | revealing the kinetics of leishmania chagasi infection in the male genital system of hamsters |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4812609/ https://www.ncbi.nlm.nih.gov/pubmed/27025459 http://dx.doi.org/10.1186/s40249-016-0122-0 |
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