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Quantitative evaluation of extended-spectrum β-lactamase-producing Escherichia coli strains in the wastewater of a French teaching hospital and relation to patient strain

BACKGROUND: Extended-spectrum β-lactamase-producing Escherichia coli has become ubiquitous and has been reported in diverse ecosystems. We evaluated the potential impact of post-acute and long-term healthcare activities on the environment by quantifying ESBL-producing Enterobacteriaceae in wastewate...

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Autores principales: Drieux, Laurence, Haenn, Sophie, Moulin, Laurent, Jarlier, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4812619/
https://www.ncbi.nlm.nih.gov/pubmed/27030806
http://dx.doi.org/10.1186/s13756-016-0108-5
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author Drieux, Laurence
Haenn, Sophie
Moulin, Laurent
Jarlier, Vincent
author_facet Drieux, Laurence
Haenn, Sophie
Moulin, Laurent
Jarlier, Vincent
author_sort Drieux, Laurence
collection PubMed
description BACKGROUND: Extended-spectrum β-lactamase-producing Escherichia coli has become ubiquitous and has been reported in diverse ecosystems. We evaluated the potential impact of post-acute and long-term healthcare activities on the environment by quantifying ESBL-producing Enterobacteriaceae in wastewaters of a French geriatric hospital. METHODS: We collected wastewater specimens representative of one-day efflux immediately before the connection with the municipal sewer pipe. The sample was processed following two different methods: dilution-filtration method and concentration method and was screened for ESBL-producing Enterobacteriaceae using selective media. ESBL E. coli strains were quantified, screened for ESBL genes and compared with ESBL strains isolated from patients present in the building at the time of wastewater collection, using molecular methods. RESULTS: Six distinct environmental ESBL E. coli clusters were identified, two of them related to patient strains. The concentrations in hospital wastewater of these strains ranged from 2.5 × 10(4) to 10(6) UFC/L. CONCLUSIONS: Our results demonstrate that the presence of ESBL E. coli patients leads to a dissemination of ESBL E. coli in the environment and highlights the need to improve excreta and wastewater policy in hospitals.
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spelling pubmed-48126192016-03-31 Quantitative evaluation of extended-spectrum β-lactamase-producing Escherichia coli strains in the wastewater of a French teaching hospital and relation to patient strain Drieux, Laurence Haenn, Sophie Moulin, Laurent Jarlier, Vincent Antimicrob Resist Infect Control Research BACKGROUND: Extended-spectrum β-lactamase-producing Escherichia coli has become ubiquitous and has been reported in diverse ecosystems. We evaluated the potential impact of post-acute and long-term healthcare activities on the environment by quantifying ESBL-producing Enterobacteriaceae in wastewaters of a French geriatric hospital. METHODS: We collected wastewater specimens representative of one-day efflux immediately before the connection with the municipal sewer pipe. The sample was processed following two different methods: dilution-filtration method and concentration method and was screened for ESBL-producing Enterobacteriaceae using selective media. ESBL E. coli strains were quantified, screened for ESBL genes and compared with ESBL strains isolated from patients present in the building at the time of wastewater collection, using molecular methods. RESULTS: Six distinct environmental ESBL E. coli clusters were identified, two of them related to patient strains. The concentrations in hospital wastewater of these strains ranged from 2.5 × 10(4) to 10(6) UFC/L. CONCLUSIONS: Our results demonstrate that the presence of ESBL E. coli patients leads to a dissemination of ESBL E. coli in the environment and highlights the need to improve excreta and wastewater policy in hospitals. BioMed Central 2016-03-29 /pmc/articles/PMC4812619/ /pubmed/27030806 http://dx.doi.org/10.1186/s13756-016-0108-5 Text en © Drieux et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Drieux, Laurence
Haenn, Sophie
Moulin, Laurent
Jarlier, Vincent
Quantitative evaluation of extended-spectrum β-lactamase-producing Escherichia coli strains in the wastewater of a French teaching hospital and relation to patient strain
title Quantitative evaluation of extended-spectrum β-lactamase-producing Escherichia coli strains in the wastewater of a French teaching hospital and relation to patient strain
title_full Quantitative evaluation of extended-spectrum β-lactamase-producing Escherichia coli strains in the wastewater of a French teaching hospital and relation to patient strain
title_fullStr Quantitative evaluation of extended-spectrum β-lactamase-producing Escherichia coli strains in the wastewater of a French teaching hospital and relation to patient strain
title_full_unstemmed Quantitative evaluation of extended-spectrum β-lactamase-producing Escherichia coli strains in the wastewater of a French teaching hospital and relation to patient strain
title_short Quantitative evaluation of extended-spectrum β-lactamase-producing Escherichia coli strains in the wastewater of a French teaching hospital and relation to patient strain
title_sort quantitative evaluation of extended-spectrum β-lactamase-producing escherichia coli strains in the wastewater of a french teaching hospital and relation to patient strain
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4812619/
https://www.ncbi.nlm.nih.gov/pubmed/27030806
http://dx.doi.org/10.1186/s13756-016-0108-5
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