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Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance

Background: Electroacupuncture (EA) tolerance is a gradual decline in EA antinociception because of its repeated or prolonged use. This study aims to explore the role of spinal glutamate transporters (GTs) in EA tolerance (EAT). Methods: Rats were treated with EA once per day for eight consecutive d...

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Detalles Bibliográficos
Autores principales: Cui, Luying, Ding, Yi, Zeng, Jie, Feng, Yan, Li, Meng, Ding, Mingxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4813218/
https://www.ncbi.nlm.nih.gov/pubmed/26978348
http://dx.doi.org/10.3390/ijms17030357
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author Cui, Luying
Ding, Yi
Zeng, Jie
Feng, Yan
Li, Meng
Ding, Mingxing
author_facet Cui, Luying
Ding, Yi
Zeng, Jie
Feng, Yan
Li, Meng
Ding, Mingxing
author_sort Cui, Luying
collection PubMed
description Background: Electroacupuncture (EA) tolerance is a gradual decline in EA antinociception because of its repeated or prolonged use. This study aims to explore the role of spinal glutamate transporters (GTs) in EA tolerance (EAT). Methods: Rats were treated with EA once per day for eight consecutive days, and their L4-5 spinal cords were collected at days 0, 2, 4, 6 and 8. The levels of three spinal GTs and their mRNAs were detected with Western blot and pPCR, respectively. Then, riluzole, a positive GT regulator, was administered intrathecally in order to observe its effect on EA analgesia after repeated EA. Results: The expression levels of the spinal GTs increased at days 2 and 4, and gradually decreased as the times of EA increased. At day 8, no difference was observed in the spinal GTs between the sham treatment and the EA treatment. Intrathecal administration of riluzole dose-dependently attenuated the decreased EA analgesia. Conclusion: These results indicated the participation of the spinal GTs in EAT.
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spelling pubmed-48132182016-04-06 Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance Cui, Luying Ding, Yi Zeng, Jie Feng, Yan Li, Meng Ding, Mingxing Int J Mol Sci Article Background: Electroacupuncture (EA) tolerance is a gradual decline in EA antinociception because of its repeated or prolonged use. This study aims to explore the role of spinal glutamate transporters (GTs) in EA tolerance (EAT). Methods: Rats were treated with EA once per day for eight consecutive days, and their L4-5 spinal cords were collected at days 0, 2, 4, 6 and 8. The levels of three spinal GTs and their mRNAs were detected with Western blot and pPCR, respectively. Then, riluzole, a positive GT regulator, was administered intrathecally in order to observe its effect on EA analgesia after repeated EA. Results: The expression levels of the spinal GTs increased at days 2 and 4, and gradually decreased as the times of EA increased. At day 8, no difference was observed in the spinal GTs between the sham treatment and the EA treatment. Intrathecal administration of riluzole dose-dependently attenuated the decreased EA analgesia. Conclusion: These results indicated the participation of the spinal GTs in EAT. MDPI 2016-03-10 /pmc/articles/PMC4813218/ /pubmed/26978348 http://dx.doi.org/10.3390/ijms17030357 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cui, Luying
Ding, Yi
Zeng, Jie
Feng, Yan
Li, Meng
Ding, Mingxing
Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance
title Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance
title_full Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance
title_fullStr Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance
title_full_unstemmed Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance
title_short Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance
title_sort spinal glutamate transporters are involved in the development of electroacupuncture tolerance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4813218/
https://www.ncbi.nlm.nih.gov/pubmed/26978348
http://dx.doi.org/10.3390/ijms17030357
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