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Alternative Glycerol Balance Strategies among Saccharomyces Species in Response to Winemaking Stress
Production and balance of glycerol is essential for the survival of yeast cells in certain stressful conditions as hyperosmotic or cold shock that occur during industrial processes as winemaking. These stress responses are well-known in S. cerevisiae, however, little is known in other phylogenetical...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814467/ https://www.ncbi.nlm.nih.gov/pubmed/27064588 http://dx.doi.org/10.3389/fmicb.2016.00435 |
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author | Pérez-Torrado, Roberto Oliveira, Bruno M. Zemančíková, Jana Sychrová, Hana Querol, Amparo |
author_facet | Pérez-Torrado, Roberto Oliveira, Bruno M. Zemančíková, Jana Sychrová, Hana Querol, Amparo |
author_sort | Pérez-Torrado, Roberto |
collection | PubMed |
description | Production and balance of glycerol is essential for the survival of yeast cells in certain stressful conditions as hyperosmotic or cold shock that occur during industrial processes as winemaking. These stress responses are well-known in S. cerevisiae, however, little is known in other phylogenetically close related Saccharomyces species associated with natural or fermentation environments such as S. uvarum, S. paradoxus or S. kudriavzevii. In this work we have investigated the expression of four genes (GPD1, GPD2, STL1, and FPS1) crucial in the glycerol pool balance in the four species with a biotechnological potential (S. cerevisiae; S. paradoxus; S. uvarum; and S. kudriavzevii), and the ability of strains to grow under osmotic and cold stresses. The results show different pattern and level of expression among the different species, especially for STL1. We also studied the function of Stl1 glycerol symporter in the survival to osmotic changes and cell growth capacity in winemaking environments. These experiments also revealed a different functionality of the glycerol transporters among the different species studied. All these data point to different strategies to handle glycerol accumulation in response to winemaking stresses as hyperosmotic or cold-hyperosmotic stress in the different species, with variable emphasis in the production, influx, or efflux of glycerol. |
format | Online Article Text |
id | pubmed-4814467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-48144672016-04-08 Alternative Glycerol Balance Strategies among Saccharomyces Species in Response to Winemaking Stress Pérez-Torrado, Roberto Oliveira, Bruno M. Zemančíková, Jana Sychrová, Hana Querol, Amparo Front Microbiol Microbiology Production and balance of glycerol is essential for the survival of yeast cells in certain stressful conditions as hyperosmotic or cold shock that occur during industrial processes as winemaking. These stress responses are well-known in S. cerevisiae, however, little is known in other phylogenetically close related Saccharomyces species associated with natural or fermentation environments such as S. uvarum, S. paradoxus or S. kudriavzevii. In this work we have investigated the expression of four genes (GPD1, GPD2, STL1, and FPS1) crucial in the glycerol pool balance in the four species with a biotechnological potential (S. cerevisiae; S. paradoxus; S. uvarum; and S. kudriavzevii), and the ability of strains to grow under osmotic and cold stresses. The results show different pattern and level of expression among the different species, especially for STL1. We also studied the function of Stl1 glycerol symporter in the survival to osmotic changes and cell growth capacity in winemaking environments. These experiments also revealed a different functionality of the glycerol transporters among the different species studied. All these data point to different strategies to handle glycerol accumulation in response to winemaking stresses as hyperosmotic or cold-hyperosmotic stress in the different species, with variable emphasis in the production, influx, or efflux of glycerol. Frontiers Media S.A. 2016-03-31 /pmc/articles/PMC4814467/ /pubmed/27064588 http://dx.doi.org/10.3389/fmicb.2016.00435 Text en Copyright © 2016 Pérez-Torrado, Oliveira, Zemančíková, Sychrová and Querol. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Pérez-Torrado, Roberto Oliveira, Bruno M. Zemančíková, Jana Sychrová, Hana Querol, Amparo Alternative Glycerol Balance Strategies among Saccharomyces Species in Response to Winemaking Stress |
title | Alternative Glycerol Balance Strategies among Saccharomyces Species in Response to Winemaking Stress |
title_full | Alternative Glycerol Balance Strategies among Saccharomyces Species in Response to Winemaking Stress |
title_fullStr | Alternative Glycerol Balance Strategies among Saccharomyces Species in Response to Winemaking Stress |
title_full_unstemmed | Alternative Glycerol Balance Strategies among Saccharomyces Species in Response to Winemaking Stress |
title_short | Alternative Glycerol Balance Strategies among Saccharomyces Species in Response to Winemaking Stress |
title_sort | alternative glycerol balance strategies among saccharomyces species in response to winemaking stress |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814467/ https://www.ncbi.nlm.nih.gov/pubmed/27064588 http://dx.doi.org/10.3389/fmicb.2016.00435 |
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