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Transcriptome Analysis of Differentially Expressed Genes Provides Insight into Stolon Formation in Tulipa edulis

Tulipa edulis (Miq.) Baker is an important medicinal plant with a variety of anti-cancer properties. The stolon is one of the main asexual reproductive organs of T. edulis and possesses a unique morphology. To explore the molecular mechanism of stolon formation, we performed an RNA-seq analysis of t...

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Autores principales: Miao, Yuanyuan, Zhu, Zaibiao, Guo, Qiaosheng, Zhu, Yunhao, Yang, Xiaohua, Sun, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814499/
https://www.ncbi.nlm.nih.gov/pubmed/27064558
http://dx.doi.org/10.3389/fpls.2016.00409
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author Miao, Yuanyuan
Zhu, Zaibiao
Guo, Qiaosheng
Zhu, Yunhao
Yang, Xiaohua
Sun, Yuan
author_facet Miao, Yuanyuan
Zhu, Zaibiao
Guo, Qiaosheng
Zhu, Yunhao
Yang, Xiaohua
Sun, Yuan
author_sort Miao, Yuanyuan
collection PubMed
description Tulipa edulis (Miq.) Baker is an important medicinal plant with a variety of anti-cancer properties. The stolon is one of the main asexual reproductive organs of T. edulis and possesses a unique morphology. To explore the molecular mechanism of stolon formation, we performed an RNA-seq analysis of the transcriptomes of stolons at three developmental stages. In the present study, 15.49 Gb of raw data were generated and assembled into 74,006 unigenes, and a total of 2,811 simple sequence repeats were detected in T. edulis. Among the three libraries of stolons at different developmental stages, there were 5,119 differentially expressed genes (DEGs). A functional annotation analysis based on sequence similarity queries of the GO, COG, KEGG databases showed that these DEGs were mainly involved in many physiological and biochemical processes, such as material and energy metabolism, hormone signaling, cell growth, and transcription regulation. In addition, quantitative real-time PCR analysis revealed that the expression patterns of the DEGs were consistent with the transcriptome data, which further supported a role for the DEGs in stolon formation. This study provides novel resources for future genetic and molecular studies in T. edulis.
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spelling pubmed-48144992016-04-08 Transcriptome Analysis of Differentially Expressed Genes Provides Insight into Stolon Formation in Tulipa edulis Miao, Yuanyuan Zhu, Zaibiao Guo, Qiaosheng Zhu, Yunhao Yang, Xiaohua Sun, Yuan Front Plant Sci Plant Science Tulipa edulis (Miq.) Baker is an important medicinal plant with a variety of anti-cancer properties. The stolon is one of the main asexual reproductive organs of T. edulis and possesses a unique morphology. To explore the molecular mechanism of stolon formation, we performed an RNA-seq analysis of the transcriptomes of stolons at three developmental stages. In the present study, 15.49 Gb of raw data were generated and assembled into 74,006 unigenes, and a total of 2,811 simple sequence repeats were detected in T. edulis. Among the three libraries of stolons at different developmental stages, there were 5,119 differentially expressed genes (DEGs). A functional annotation analysis based on sequence similarity queries of the GO, COG, KEGG databases showed that these DEGs were mainly involved in many physiological and biochemical processes, such as material and energy metabolism, hormone signaling, cell growth, and transcription regulation. In addition, quantitative real-time PCR analysis revealed that the expression patterns of the DEGs were consistent with the transcriptome data, which further supported a role for the DEGs in stolon formation. This study provides novel resources for future genetic and molecular studies in T. edulis. Frontiers Media S.A. 2016-03-31 /pmc/articles/PMC4814499/ /pubmed/27064558 http://dx.doi.org/10.3389/fpls.2016.00409 Text en Copyright © 2016 Miao, Zhu, Guo, Zhu, Yang and Sun. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Miao, Yuanyuan
Zhu, Zaibiao
Guo, Qiaosheng
Zhu, Yunhao
Yang, Xiaohua
Sun, Yuan
Transcriptome Analysis of Differentially Expressed Genes Provides Insight into Stolon Formation in Tulipa edulis
title Transcriptome Analysis of Differentially Expressed Genes Provides Insight into Stolon Formation in Tulipa edulis
title_full Transcriptome Analysis of Differentially Expressed Genes Provides Insight into Stolon Formation in Tulipa edulis
title_fullStr Transcriptome Analysis of Differentially Expressed Genes Provides Insight into Stolon Formation in Tulipa edulis
title_full_unstemmed Transcriptome Analysis of Differentially Expressed Genes Provides Insight into Stolon Formation in Tulipa edulis
title_short Transcriptome Analysis of Differentially Expressed Genes Provides Insight into Stolon Formation in Tulipa edulis
title_sort transcriptome analysis of differentially expressed genes provides insight into stolon formation in tulipa edulis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814499/
https://www.ncbi.nlm.nih.gov/pubmed/27064558
http://dx.doi.org/10.3389/fpls.2016.00409
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