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Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize
Doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. To test if haploid inducer lines can be engineered into crops, CENH3(−∕−) and CENH3:RNAi lines were complemented by AcGREEN-tailswap-CENH3 or AcGREEN-CENH3 transgene...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814585/ https://www.ncbi.nlm.nih.gov/pubmed/27066050 http://dx.doi.org/10.3389/fpls.2016.00414 |
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author | Kelliher, Timothy Starr, Dakota Wang, Wenling McCuiston, Jamie Zhong, Heng Nuccio, Michael L. Martin, Barry |
author_facet | Kelliher, Timothy Starr, Dakota Wang, Wenling McCuiston, Jamie Zhong, Heng Nuccio, Michael L. Martin, Barry |
author_sort | Kelliher, Timothy |
collection | PubMed |
description | Doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. To test if haploid inducer lines can be engineered into crops, CENH3(−∕−) and CENH3:RNAi lines were complemented by AcGREEN-tailswap-CENH3 or AcGREEN-CENH3 transgenes. Haploid induction rates were determined following testcrosses to wild-type plants after independently controlling for inducer parent sex and transgene zygosity. CENH3 fusion proteins were localized to centromeres and did not cause vegetative defects or male sterility. CENH3:RNAi lines did not demonstrate consistent knockdown and rarely produced haploids. In contrast, many of the complemented CENH3(−∕−) lines produced haploids at low frequencies. The rate of gynogenic haploid induction reached a maximum of 3.6% in several hemizygous individuals when backcrossed as males. These results demonstrate that CENH3-tailswap transgenes can be used to engineer in vivo haploid induction systems into maize plants. |
format | Online Article Text |
id | pubmed-4814585 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-48145852016-04-08 Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize Kelliher, Timothy Starr, Dakota Wang, Wenling McCuiston, Jamie Zhong, Heng Nuccio, Michael L. Martin, Barry Front Plant Sci Plant Science Doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. To test if haploid inducer lines can be engineered into crops, CENH3(−∕−) and CENH3:RNAi lines were complemented by AcGREEN-tailswap-CENH3 or AcGREEN-CENH3 transgenes. Haploid induction rates were determined following testcrosses to wild-type plants after independently controlling for inducer parent sex and transgene zygosity. CENH3 fusion proteins were localized to centromeres and did not cause vegetative defects or male sterility. CENH3:RNAi lines did not demonstrate consistent knockdown and rarely produced haploids. In contrast, many of the complemented CENH3(−∕−) lines produced haploids at low frequencies. The rate of gynogenic haploid induction reached a maximum of 3.6% in several hemizygous individuals when backcrossed as males. These results demonstrate that CENH3-tailswap transgenes can be used to engineer in vivo haploid induction systems into maize plants. Frontiers Media S.A. 2016-03-31 /pmc/articles/PMC4814585/ /pubmed/27066050 http://dx.doi.org/10.3389/fpls.2016.00414 Text en Copyright © 2016 Kelliher, Starr, Wang, McCuiston, Zhong, Nuccio and Martin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Kelliher, Timothy Starr, Dakota Wang, Wenling McCuiston, Jamie Zhong, Heng Nuccio, Michael L. Martin, Barry Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize |
title | Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize |
title_full | Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize |
title_fullStr | Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize |
title_full_unstemmed | Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize |
title_short | Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize |
title_sort | maternal haploids are preferentially induced by cenh3-tailswap transgenic complementation in maize |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814585/ https://www.ncbi.nlm.nih.gov/pubmed/27066050 http://dx.doi.org/10.3389/fpls.2016.00414 |
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