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Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation

Rab GTPases have been reported to define the identity and transport routes of vesicles. Rab6 is one of the most extensively studied Rab proteins involved in regulating organelle trafficking and integrity maintenance. However, to date, the function of Rab6 in mammalian oocytes has not been addressed....

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Autores principales: Ma, Rujun, Zhang, Jiaqi, Liu, Xiaohui, Li, Ling, Liu, Honglin, Rui, Rong, Gu, Ling, Wang, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814827/
https://www.ncbi.nlm.nih.gov/pubmed/27030207
http://dx.doi.org/10.1038/srep23560
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author Ma, Rujun
Zhang, Jiaqi
Liu, Xiaohui
Li, Ling
Liu, Honglin
Rui, Rong
Gu, Ling
Wang, Qiang
author_facet Ma, Rujun
Zhang, Jiaqi
Liu, Xiaohui
Li, Ling
Liu, Honglin
Rui, Rong
Gu, Ling
Wang, Qiang
author_sort Ma, Rujun
collection PubMed
description Rab GTPases have been reported to define the identity and transport routes of vesicles. Rab6 is one of the most extensively studied Rab proteins involved in regulating organelle trafficking and integrity maintenance. However, to date, the function of Rab6 in mammalian oocytes has not been addressed. Here we report severe disorganization of endoplasmic reticulum upon specific knockdown of Rab6a in mouse oocytes. In line with this finding, intracellular Ca(2+) stores are accordingly reduced in Rab6a-depleted oocytes. Furthermore, in these oocytes, we observe the absence of cortical granule free domain, which is a kind of special organelle in matured oocytes and its exocytosis is calcium dependent. On the other hand, following Rab6a knockdown, the prominent defects of cytoskeletal structures are detected during oocyte meiosis. In particular, the majority of Rab6a-depleted oocytes fail to form the actin cap, and the frequency of spindle defects and chromosome misalignment is significantly elevated. In summary, our data reveal that Rab6a not only participates in modulating the organization of oocyte organelles, but also is a novel regulator of meiotic apparatus in mammalian oocytes.
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spelling pubmed-48148272016-04-04 Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation Ma, Rujun Zhang, Jiaqi Liu, Xiaohui Li, Ling Liu, Honglin Rui, Rong Gu, Ling Wang, Qiang Sci Rep Article Rab GTPases have been reported to define the identity and transport routes of vesicles. Rab6 is one of the most extensively studied Rab proteins involved in regulating organelle trafficking and integrity maintenance. However, to date, the function of Rab6 in mammalian oocytes has not been addressed. Here we report severe disorganization of endoplasmic reticulum upon specific knockdown of Rab6a in mouse oocytes. In line with this finding, intracellular Ca(2+) stores are accordingly reduced in Rab6a-depleted oocytes. Furthermore, in these oocytes, we observe the absence of cortical granule free domain, which is a kind of special organelle in matured oocytes and its exocytosis is calcium dependent. On the other hand, following Rab6a knockdown, the prominent defects of cytoskeletal structures are detected during oocyte meiosis. In particular, the majority of Rab6a-depleted oocytes fail to form the actin cap, and the frequency of spindle defects and chromosome misalignment is significantly elevated. In summary, our data reveal that Rab6a not only participates in modulating the organization of oocyte organelles, but also is a novel regulator of meiotic apparatus in mammalian oocytes. Nature Publishing Group 2016-03-31 /pmc/articles/PMC4814827/ /pubmed/27030207 http://dx.doi.org/10.1038/srep23560 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ma, Rujun
Zhang, Jiaqi
Liu, Xiaohui
Li, Ling
Liu, Honglin
Rui, Rong
Gu, Ling
Wang, Qiang
Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation
title Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation
title_full Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation
title_fullStr Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation
title_full_unstemmed Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation
title_short Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation
title_sort involvement of rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814827/
https://www.ncbi.nlm.nih.gov/pubmed/27030207
http://dx.doi.org/10.1038/srep23560
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