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Dysregulated 14-3-3 Family in Peripheral Blood Leukocytes of Patients with Schizophrenia
The 14-3-3 family, which is composed of seven distinct members in humans, plays important roles in the cell cycle, apoptosis, synaptic plasticity and neuronal differentiation and migration. Previous genetic and post-mortem gene expression studies have linked this family to schizophrenia. However, th...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814835/ https://www.ncbi.nlm.nih.gov/pubmed/27030512 http://dx.doi.org/10.1038/srep23791 |
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author | Qing, Ying Sun, Liya Yang, Chao Jiang, Jie Yang, Xuhan Hu, Xiaowen Cui, Donghong Xu, Yifeng He, Lin Han, Dongmei Wan, Chunling |
author_facet | Qing, Ying Sun, Liya Yang, Chao Jiang, Jie Yang, Xuhan Hu, Xiaowen Cui, Donghong Xu, Yifeng He, Lin Han, Dongmei Wan, Chunling |
author_sort | Qing, Ying |
collection | PubMed |
description | The 14-3-3 family, which is composed of seven distinct members in humans, plays important roles in the cell cycle, apoptosis, synaptic plasticity and neuronal differentiation and migration. Previous genetic and post-mortem gene expression studies have linked this family to schizophrenia. However, the direction of gene expression changes in these studies has been inconsistent, and reports of 14-3-3 gene expression in living schizophrenic patients are still lacking. Here, we assessed 14-3-3 gene and protein expression levels in peripheral blood leukocytes from drug-naïve first-episode schizophrenic patients and matched controls. mRNA and protein expression levels were quantified by qRT-PCR and UPLC-MRM/MS, respectively. Expression analysis revealed four downregulated and one upregulated mRNA transcripts as well as five downregulated protein levels of 14-3-3 isoforms in schizophrenia. Moreover, significant positive correlations between 14-3-3 mRNA and protein expression levels were found in schizophrenia, and we also identified negative correlations between ε, θ and ζ isoform expression levels and positive symptoms of schizophrenia. Our results suggest that gene and protein expression levels for the 14-3-3 family are dysregulated in schizophrenia, perhaps owing to specific regulatory mechanisms, and we also suggest that expression of the 14-3-3ε, θ and ζ isoform genes could be useful indicators of disease severity. |
format | Online Article Text |
id | pubmed-4814835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48148352016-04-04 Dysregulated 14-3-3 Family in Peripheral Blood Leukocytes of Patients with Schizophrenia Qing, Ying Sun, Liya Yang, Chao Jiang, Jie Yang, Xuhan Hu, Xiaowen Cui, Donghong Xu, Yifeng He, Lin Han, Dongmei Wan, Chunling Sci Rep Article The 14-3-3 family, which is composed of seven distinct members in humans, plays important roles in the cell cycle, apoptosis, synaptic plasticity and neuronal differentiation and migration. Previous genetic and post-mortem gene expression studies have linked this family to schizophrenia. However, the direction of gene expression changes in these studies has been inconsistent, and reports of 14-3-3 gene expression in living schizophrenic patients are still lacking. Here, we assessed 14-3-3 gene and protein expression levels in peripheral blood leukocytes from drug-naïve first-episode schizophrenic patients and matched controls. mRNA and protein expression levels were quantified by qRT-PCR and UPLC-MRM/MS, respectively. Expression analysis revealed four downregulated and one upregulated mRNA transcripts as well as five downregulated protein levels of 14-3-3 isoforms in schizophrenia. Moreover, significant positive correlations between 14-3-3 mRNA and protein expression levels were found in schizophrenia, and we also identified negative correlations between ε, θ and ζ isoform expression levels and positive symptoms of schizophrenia. Our results suggest that gene and protein expression levels for the 14-3-3 family are dysregulated in schizophrenia, perhaps owing to specific regulatory mechanisms, and we also suggest that expression of the 14-3-3ε, θ and ζ isoform genes could be useful indicators of disease severity. Nature Publishing Group 2016-03-31 /pmc/articles/PMC4814835/ /pubmed/27030512 http://dx.doi.org/10.1038/srep23791 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Qing, Ying Sun, Liya Yang, Chao Jiang, Jie Yang, Xuhan Hu, Xiaowen Cui, Donghong Xu, Yifeng He, Lin Han, Dongmei Wan, Chunling Dysregulated 14-3-3 Family in Peripheral Blood Leukocytes of Patients with Schizophrenia |
title | Dysregulated 14-3-3 Family in Peripheral Blood Leukocytes of Patients with Schizophrenia |
title_full | Dysregulated 14-3-3 Family in Peripheral Blood Leukocytes of Patients with Schizophrenia |
title_fullStr | Dysregulated 14-3-3 Family in Peripheral Blood Leukocytes of Patients with Schizophrenia |
title_full_unstemmed | Dysregulated 14-3-3 Family in Peripheral Blood Leukocytes of Patients with Schizophrenia |
title_short | Dysregulated 14-3-3 Family in Peripheral Blood Leukocytes of Patients with Schizophrenia |
title_sort | dysregulated 14-3-3 family in peripheral blood leukocytes of patients with schizophrenia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814835/ https://www.ncbi.nlm.nih.gov/pubmed/27030512 http://dx.doi.org/10.1038/srep23791 |
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