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Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing

Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reducing the rate of high-fidelity edits. The identific...

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Detalles Bibliográficos
Autores principales:	Miyaoka, Yuichiro, Berman, Jennifer R., Cooper, Samantha B., Mayerl, Steven J., Chan, Amanda H., Zhang, Bin, Karlin-Neumann, George A., Conklin, Bruce R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814844/ https://www.ncbi.nlm.nih.gov/pubmed/27030102 http://dx.doi.org/10.1038/srep23549

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