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Sex reversal assessments reveal different vulnerability to endocrine disruption between deeply diverged anuran lineages

Multiple anthropogenic stressors cause worldwide amphibian declines. Among several poorly investigated causes is global pollution of aquatic ecosystems with endocrine disrupting compounds (EDCs). These substances interfere with the endocrine system and can affect the sexual development of vertebrate...

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Autores principales: Tamschick, Stephanie, Rozenblut-Kościsty, Beata, Ogielska, Maria, Lehmann, Andreas, Lymberakis, Petros, Hoffmann, Frauke, Lutz, Ilka, Kloas, Werner, Stöck, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814869/
https://www.ncbi.nlm.nih.gov/pubmed/27029458
http://dx.doi.org/10.1038/srep23825
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author Tamschick, Stephanie
Rozenblut-Kościsty, Beata
Ogielska, Maria
Lehmann, Andreas
Lymberakis, Petros
Hoffmann, Frauke
Lutz, Ilka
Kloas, Werner
Stöck, Matthias
author_facet Tamschick, Stephanie
Rozenblut-Kościsty, Beata
Ogielska, Maria
Lehmann, Andreas
Lymberakis, Petros
Hoffmann, Frauke
Lutz, Ilka
Kloas, Werner
Stöck, Matthias
author_sort Tamschick, Stephanie
collection PubMed
description Multiple anthropogenic stressors cause worldwide amphibian declines. Among several poorly investigated causes is global pollution of aquatic ecosystems with endocrine disrupting compounds (EDCs). These substances interfere with the endocrine system and can affect the sexual development of vertebrates including amphibians. We test the susceptibility to an environmentally relevant contraceptive, the artificial estrogen 17α-ethinylestradiol (EE2), simultaneously in three deeply divergent systematic anuran families, a model-species, Xenopus laevis (Pipidae), and two non-models, Hyla arborea (Hylidae) and Bufo viridis (Bufonidae). Our new approach combines synchronized tadpole exposure to three EE2-concentrations (50, 500, 5,000 ng/L) in a flow-through-system and pioneers genetic and histological sexing of metamorphs in non-model anurans for EDC-studies. This novel methodology reveals striking quantitative differences in genetic-male-to-phenotypic-female sex reversal in non-model vs. model species. Our findings qualify molecular sexing in EDC-analyses as requirement to identify sex reversals and state-of-the-art approaches as mandatory to detect species-specific vulnerabilities to EDCs in amphibians.
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spelling pubmed-48148692016-04-04 Sex reversal assessments reveal different vulnerability to endocrine disruption between deeply diverged anuran lineages Tamschick, Stephanie Rozenblut-Kościsty, Beata Ogielska, Maria Lehmann, Andreas Lymberakis, Petros Hoffmann, Frauke Lutz, Ilka Kloas, Werner Stöck, Matthias Sci Rep Article Multiple anthropogenic stressors cause worldwide amphibian declines. Among several poorly investigated causes is global pollution of aquatic ecosystems with endocrine disrupting compounds (EDCs). These substances interfere with the endocrine system and can affect the sexual development of vertebrates including amphibians. We test the susceptibility to an environmentally relevant contraceptive, the artificial estrogen 17α-ethinylestradiol (EE2), simultaneously in three deeply divergent systematic anuran families, a model-species, Xenopus laevis (Pipidae), and two non-models, Hyla arborea (Hylidae) and Bufo viridis (Bufonidae). Our new approach combines synchronized tadpole exposure to three EE2-concentrations (50, 500, 5,000 ng/L) in a flow-through-system and pioneers genetic and histological sexing of metamorphs in non-model anurans for EDC-studies. This novel methodology reveals striking quantitative differences in genetic-male-to-phenotypic-female sex reversal in non-model vs. model species. Our findings qualify molecular sexing in EDC-analyses as requirement to identify sex reversals and state-of-the-art approaches as mandatory to detect species-specific vulnerabilities to EDCs in amphibians. Nature Publishing Group 2016-03-31 /pmc/articles/PMC4814869/ /pubmed/27029458 http://dx.doi.org/10.1038/srep23825 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tamschick, Stephanie
Rozenblut-Kościsty, Beata
Ogielska, Maria
Lehmann, Andreas
Lymberakis, Petros
Hoffmann, Frauke
Lutz, Ilka
Kloas, Werner
Stöck, Matthias
Sex reversal assessments reveal different vulnerability to endocrine disruption between deeply diverged anuran lineages
title Sex reversal assessments reveal different vulnerability to endocrine disruption between deeply diverged anuran lineages
title_full Sex reversal assessments reveal different vulnerability to endocrine disruption between deeply diverged anuran lineages
title_fullStr Sex reversal assessments reveal different vulnerability to endocrine disruption between deeply diverged anuran lineages
title_full_unstemmed Sex reversal assessments reveal different vulnerability to endocrine disruption between deeply diverged anuran lineages
title_short Sex reversal assessments reveal different vulnerability to endocrine disruption between deeply diverged anuran lineages
title_sort sex reversal assessments reveal different vulnerability to endocrine disruption between deeply diverged anuran lineages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814869/
https://www.ncbi.nlm.nih.gov/pubmed/27029458
http://dx.doi.org/10.1038/srep23825
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