Cargando…

An assessment of the usefulness of a rapid immuno-chromatographic test, "Determine™ malaria pf" in evaluation of intervention measures in forest villages of central India

BACKGROUND: Plasmodium falciparum malaria, is a major health problem in forested tribal belt of central India. Rapid and accurate methods are needed for the diagnosis of P. falciparum. We performed a blinded evaluation of the recently introduced Determine™ malaria pf test (Abbott, Laboratories, Japa...

Descripción completa

Detalles Bibliográficos
Autores principales: Singh, Neeru, Shukla, Manmohan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC48149/
https://www.ncbi.nlm.nih.gov/pubmed/11532200
http://dx.doi.org/10.1186/1471-2334-1-10
_version_ 1782120027509489664
author Singh, Neeru
Shukla, Manmohan
author_facet Singh, Neeru
Shukla, Manmohan
author_sort Singh, Neeru
collection PubMed
description BACKGROUND: Plasmodium falciparum malaria, is a major health problem in forested tribal belt of central India. Rapid and accurate methods are needed for the diagnosis of P. falciparum. We performed a blinded evaluation of the recently introduced Determine™ malaria pf test (Abbott, Laboratories, Japan) compared with microscopy and splenomegaly in children in epidemic prone areas of district Mandla to assess the impact of intervention measures. METHODS: Children aged 2–10 yrs with and without fever were examined for spleen enlargement by medical specialist by establishing a mobile field clinic. From these children thick blood smears were prepared from finger prick and read by a technician. Simultaneously, rapid tests were performed by a field lab attendant. The figures for specificity, sensitivity and predictive values were calculated using microscopy as gold standard. RESULTS: In all 349 children were examined. The sensitivity and specificity for Determine rapid diagnostic test were 91 and 80% respectively. The positive predictive values (PPV), negative predictive values (NPV) and accuracy of the test were respectively 79, 91 and 85%. On the contrary, the sensitivity and specificity of spleen in detecting malaria infection were 57 and 74 % respectively with PPV of 73%, NPV 59 % and an accuracy of 65%. CONCLUSIONS: Determine™ malaria rapid diagnostic test is easier and quicker to perform and has other advantages over microscopy in not requiring prior training of personnel or quality control. Thus, highlighting the usefulness of a rapid antigen test in assessing prevailing malaria situation in remote areas.
format Text
id pubmed-48149
institution National Center for Biotechnology Information
language English
publishDate 2001
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-481492001-09-04 An assessment of the usefulness of a rapid immuno-chromatographic test, "Determine™ malaria pf" in evaluation of intervention measures in forest villages of central India Singh, Neeru Shukla, Manmohan BMC Infect Dis Research Article BACKGROUND: Plasmodium falciparum malaria, is a major health problem in forested tribal belt of central India. Rapid and accurate methods are needed for the diagnosis of P. falciparum. We performed a blinded evaluation of the recently introduced Determine™ malaria pf test (Abbott, Laboratories, Japan) compared with microscopy and splenomegaly in children in epidemic prone areas of district Mandla to assess the impact of intervention measures. METHODS: Children aged 2–10 yrs with and without fever were examined for spleen enlargement by medical specialist by establishing a mobile field clinic. From these children thick blood smears were prepared from finger prick and read by a technician. Simultaneously, rapid tests were performed by a field lab attendant. The figures for specificity, sensitivity and predictive values were calculated using microscopy as gold standard. RESULTS: In all 349 children were examined. The sensitivity and specificity for Determine rapid diagnostic test were 91 and 80% respectively. The positive predictive values (PPV), negative predictive values (NPV) and accuracy of the test were respectively 79, 91 and 85%. On the contrary, the sensitivity and specificity of spleen in detecting malaria infection were 57 and 74 % respectively with PPV of 73%, NPV 59 % and an accuracy of 65%. CONCLUSIONS: Determine™ malaria rapid diagnostic test is easier and quicker to perform and has other advantages over microscopy in not requiring prior training of personnel or quality control. Thus, highlighting the usefulness of a rapid antigen test in assessing prevailing malaria situation in remote areas. BioMed Central 2001-08-14 /pmc/articles/PMC48149/ /pubmed/11532200 http://dx.doi.org/10.1186/1471-2334-1-10 Text en Copyright © 2001 Singh and Shukla; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Singh, Neeru
Shukla, Manmohan
An assessment of the usefulness of a rapid immuno-chromatographic test, "Determine™ malaria pf" in evaluation of intervention measures in forest villages of central India
title An assessment of the usefulness of a rapid immuno-chromatographic test, "Determine™ malaria pf" in evaluation of intervention measures in forest villages of central India
title_full An assessment of the usefulness of a rapid immuno-chromatographic test, "Determine™ malaria pf" in evaluation of intervention measures in forest villages of central India
title_fullStr An assessment of the usefulness of a rapid immuno-chromatographic test, "Determine™ malaria pf" in evaluation of intervention measures in forest villages of central India
title_full_unstemmed An assessment of the usefulness of a rapid immuno-chromatographic test, "Determine™ malaria pf" in evaluation of intervention measures in forest villages of central India
title_short An assessment of the usefulness of a rapid immuno-chromatographic test, "Determine™ malaria pf" in evaluation of intervention measures in forest villages of central India
title_sort assessment of the usefulness of a rapid immuno-chromatographic test, "determine™ malaria pf" in evaluation of intervention measures in forest villages of central india
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC48149/
https://www.ncbi.nlm.nih.gov/pubmed/11532200
http://dx.doi.org/10.1186/1471-2334-1-10
work_keys_str_mv AT singhneeru anassessmentoftheusefulnessofarapidimmunochromatographictestdeterminemalariapfinevaluationofinterventionmeasuresinforestvillagesofcentralindia
AT shuklamanmohan anassessmentoftheusefulnessofarapidimmunochromatographictestdeterminemalariapfinevaluationofinterventionmeasuresinforestvillagesofcentralindia
AT singhneeru assessmentoftheusefulnessofarapidimmunochromatographictestdeterminemalariapfinevaluationofinterventionmeasuresinforestvillagesofcentralindia
AT shuklamanmohan assessmentoftheusefulnessofarapidimmunochromatographictestdeterminemalariapfinevaluationofinterventionmeasuresinforestvillagesofcentralindia