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A Novel Typing Method for Streptococcus pneumoniae Using Selected Surface Proteins

The diverse pneumococcal diseases are associated with different pneumococcal lineages, or clonal complexes. Nevertheless, intra-clonal genomic variability, which influences pathogenicity, has been reported for surface virulence factors. These factors constitute the communication interface between th...

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Autores principales: Domenech, Arnau, Moreno, Javier, Ardanuy, Carmen, Liñares, Josefina, de la Campa, Adela G., Martin-Galiano, Antonio J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815138/
https://www.ncbi.nlm.nih.gov/pubmed/27064593
http://dx.doi.org/10.3389/fmicb.2016.00420
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author Domenech, Arnau
Moreno, Javier
Ardanuy, Carmen
Liñares, Josefina
de la Campa, Adela G.
Martin-Galiano, Antonio J.
author_facet Domenech, Arnau
Moreno, Javier
Ardanuy, Carmen
Liñares, Josefina
de la Campa, Adela G.
Martin-Galiano, Antonio J.
author_sort Domenech, Arnau
collection PubMed
description The diverse pneumococcal diseases are associated with different pneumococcal lineages, or clonal complexes. Nevertheless, intra-clonal genomic variability, which influences pathogenicity, has been reported for surface virulence factors. These factors constitute the communication interface between the pathogen and its host and their corresponding genes are subjected to strong selective pressures affecting functionality and immunogenicity. First, the presence and allelic dispersion of 97 outer protein families were screened in 19 complete pneumococcal genomes. Seventeen families were deemed variable and were then examined in 216 draft genomes. This procedure allowed the generation of binary vectors with 17 positions and the classification of strains into surfotypes. They represent the outer protein subsets with the highest inter-strain discriminative power. A total of 116 non-redundant surfotypes were identified. Those sharing a critical number of common protein features were hierarchically clustered into 18 surfogroups. Most clonal complexes with comparable epidemiological characteristics belonged to the same or similar surfogroups. However, the very large CC156 clonal complex was dispersed over several surfogroups. In order to establish a relationship between surfogroup and pathogenicity, the surfotypes of 95 clinical isolates with different serogroup/serotype combinations were analyzed. We found a significant correlation between surfogroup and type of pathogenic behavior (primary invasive, opportunistic invasive, and non-invasive). We conclude that the virulent behavior of S. pneumoniae is related to the activity of collections of, rather than individual, surface virulence factors. Since surfotypes evolve faster than MLSTs and directly reflect virulence potential, this novel typing protocol is appropriate for the identification of emerging clones.
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spelling pubmed-48151382016-04-08 A Novel Typing Method for Streptococcus pneumoniae Using Selected Surface Proteins Domenech, Arnau Moreno, Javier Ardanuy, Carmen Liñares, Josefina de la Campa, Adela G. Martin-Galiano, Antonio J. Front Microbiol Public Health The diverse pneumococcal diseases are associated with different pneumococcal lineages, or clonal complexes. Nevertheless, intra-clonal genomic variability, which influences pathogenicity, has been reported for surface virulence factors. These factors constitute the communication interface between the pathogen and its host and their corresponding genes are subjected to strong selective pressures affecting functionality and immunogenicity. First, the presence and allelic dispersion of 97 outer protein families were screened in 19 complete pneumococcal genomes. Seventeen families were deemed variable and were then examined in 216 draft genomes. This procedure allowed the generation of binary vectors with 17 positions and the classification of strains into surfotypes. They represent the outer protein subsets with the highest inter-strain discriminative power. A total of 116 non-redundant surfotypes were identified. Those sharing a critical number of common protein features were hierarchically clustered into 18 surfogroups. Most clonal complexes with comparable epidemiological characteristics belonged to the same or similar surfogroups. However, the very large CC156 clonal complex was dispersed over several surfogroups. In order to establish a relationship between surfogroup and pathogenicity, the surfotypes of 95 clinical isolates with different serogroup/serotype combinations were analyzed. We found a significant correlation between surfogroup and type of pathogenic behavior (primary invasive, opportunistic invasive, and non-invasive). We conclude that the virulent behavior of S. pneumoniae is related to the activity of collections of, rather than individual, surface virulence factors. Since surfotypes evolve faster than MLSTs and directly reflect virulence potential, this novel typing protocol is appropriate for the identification of emerging clones. Frontiers Media S.A. 2016-03-31 /pmc/articles/PMC4815138/ /pubmed/27064593 http://dx.doi.org/10.3389/fmicb.2016.00420 Text en Copyright © 2016 Domenech, Moreno, Ardanuy, Liñares, de la Campa and Martin-Galiano. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Domenech, Arnau
Moreno, Javier
Ardanuy, Carmen
Liñares, Josefina
de la Campa, Adela G.
Martin-Galiano, Antonio J.
A Novel Typing Method for Streptococcus pneumoniae Using Selected Surface Proteins
title A Novel Typing Method for Streptococcus pneumoniae Using Selected Surface Proteins
title_full A Novel Typing Method for Streptococcus pneumoniae Using Selected Surface Proteins
title_fullStr A Novel Typing Method for Streptococcus pneumoniae Using Selected Surface Proteins
title_full_unstemmed A Novel Typing Method for Streptococcus pneumoniae Using Selected Surface Proteins
title_short A Novel Typing Method for Streptococcus pneumoniae Using Selected Surface Proteins
title_sort novel typing method for streptococcus pneumoniae using selected surface proteins
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815138/
https://www.ncbi.nlm.nih.gov/pubmed/27064593
http://dx.doi.org/10.3389/fmicb.2016.00420
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